Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANPE0004(Antiviral peptide)

DFBP ID	DFBPANPE0004
Peptide sequence	LLEY
Type	Native peptide
Peptide/Function name	Antiviral peptide

Function-activity relationship

Main bioactivity	Antiviral activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Leu-Glu-Tyr

Single-letter amino acid

LLEY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	536.61 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.7000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Oyster
Precursor protein	Oyster proteins
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1013 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase 1B Source.2: DFBPPR1014 ---- Plant proteins ---- Flap endonuclease GEN-like 1 Source.3: DFBPPR1213 ---- Plant proteins ---- Chromatin assembly factor 1 subunit FSM Source.4: DFBPPR1219 ---- Plant proteins ---- Guanylate kinase 2, chloroplastic/mitochondrial Source.5: DFBPPR1637 ---- Plant proteins ---- Ent-copalyl diphosphate synthase 2 Source.6: DFBPPR1963 ---- Plant proteins ---- Potassium channel AKT1 Source.7: DFBPPR1981 ---- Plant proteins ---- Signal peptide peptidase 2 Source.8: DFBPPR2195 ---- Plant proteins ---- Signal peptide peptidase 1 Source.9: DFBPPR2385 ---- Plant proteins ---- Cyclin-A1-1 Source.10: DFBPPR2503 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A Source.11: DFBPPR2977 ---- Plant proteins ---- Nicotinate-nucleotide pyrophosphorylase [carboxylating], chloroplastic Source.12: DFBPPR3000 ---- Plant proteins ---- Kinesin-like protein KIN-14L Source.13: DFBPPR3199 ---- Plant proteins ---- Cyclin-B1-3 Source.14: DFBPPR3248 ---- Plant proteins ---- Endoglucanase 16 Source.15: DFBPPR3396 ---- Plant proteins ---- Probable transcription factor GLK2 Source.16: DFBPPR3494 ---- Plant proteins ---- Probable E3 ubiquitin-protein ligase XBOS32 Source.17: DFBPPR3574 ---- Plant proteins ---- Putative protein phosphatase 2C 76 Source.18: DFBPPR3644 ---- Plant proteins ---- Chaperone protein ClpC3, chloroplastic Source.19: DFBPPR3646 ---- Plant proteins ---- Cyclin-A1-3 Source.20: DFBPPR3707 ---- Plant proteins ---- Probable indole-3-acetic acid-amido synthetase GH3.11 Source.21: DFBPPR3894 ---- Plant proteins ---- Cyclin-A3-1 Source.22: DFBPPR3907 ---- Plant proteins ---- Cyclin-A1-4 Source.23: DFBPPR3932 ---- Plant proteins ---- Cyclin-A1-2 Source.24: DFBPPR4089 ---- Plant proteins ---- Dehydration-responsive element-binding protein 1D Source.25: DFBPPR4130 ---- Plant proteins ---- Two-component response regulator-like PRR95 Source.26: DFBPPR5601 ---- Plant proteins ---- Protein HIRA Source.27: DFBPPR5913 ---- Plant proteins ---- Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic Source.28: DFBPPR5946 ---- Plant proteins ---- Maturase K Source.29: DFBPPR6247 ---- Plant proteins ---- DNA topoisomerase 2 Source.30: DFBPPR6368 ---- Plant proteins ---- Provicilin Source.31: DFBPPR6470 ---- Plant proteins ---- Vicilin Source.32: DFBPPR7165 ---- Plant proteins ---- Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic Source.33: DFBPPR7632 ---- Milk proteins ---- Receptor tyrosine-protein kinase erbB-4 Source.34: DFBPPR15985 ---- Animal proteins ---- von Willebrand factor Source.35: DFBPPR16027 ---- Animal proteins ---- Rho GTPase-activating protein 35 Source.36: DFBPPR16206 ---- Animal proteins ---- Glutamate decarboxylase 2 Source.37: DFBPPR16219 ---- Animal proteins ---- Sodium channel protein type 10 subunit alpha Source.38: DFBPPR16252 ---- Animal proteins ---- Steroid hormone receptor ERR1 Source.39: DFBPPR16290 ---- Animal proteins ---- Cytochrome P450 2C21 Source.40: DFBPPR16575 ---- Animal proteins ---- Dystrophin Source.41: DFBPPR16748 ---- Animal proteins ---- Thyroid adenoma-associated protein homolog Source.42: DFBPPR16788 ---- Animal proteins ---- Alpha-crystallin A chain Source.43: DFBPPR16875 ---- Animal proteins ---- von Willebrand factor Source.44: DFBPPR16956 ---- Animal proteins ---- Interleukin-6 Source.45: DFBPPR17029 ---- Animal proteins ---- Cytochrome b-245 light chain Source.46: DFBPPR17057 ---- Animal proteins ---- Cation-independent mannose-6-phosphate receptor Source.47: DFBPPR17066 ---- Animal proteins ---- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Source.48: DFBPPR17130 ---- Animal proteins ---- Glycine receptor subunit alpha-1 Source.49: DFBPPR17397 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit beta-2 Source.50: DFBPPR17412 ---- Animal proteins ---- Fragile X mental retardation syndrome-related protein 1 Source.51: DFBPPR17492 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit beta-1 Source.52: DFBPPR17508 ---- Animal proteins ---- General transcription and DNA repair factor IIH helicase subunit XPD Source.53: DFBPPR17899 ---- Animal proteins ---- Eukaryotic translation initiation factor 2 subunit 1 Source.54: DFBPPR17914 ---- Animal proteins ---- Glycerol-3-phosphate acyltransferase 1, mitochondrial Source.55: DFBPPR17957 ---- Animal proteins ---- E3 ubiquitin-protein ligase HACE1 Source.56: DFBPPR18061 ---- Animal proteins ---- Glutathione S-transferase Mu 1 Source.57: DFBPPR18216 ---- Animal proteins ---- Collectin-12 Source.58: DFBPPR18243 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit pi Source.59: DFBPPR18360 ---- Animal proteins ---- Desmocollin-3 Source.60: DFBPPR18567 ---- Animal proteins ---- Dynein heavy chain 12, axonemal Source.61: DFBPPR18788 ---- Animal proteins ---- Ceramide-1-phosphate transfer protein Source.62: DFBPPR19253 ---- Animal proteins ---- Limbin Source.63: DFBPPR19330 ---- Animal proteins ---- Nuclear pore complex protein Nup85 Source.64: DFBPPR19612 ---- Animal proteins ---- Lysosomal-trafficking regulator Source.65: DFBPPR20082 ---- Animal proteins ---- Calcium-binding and coiled-coil domain-containing protein 1 Source.66: DFBPPR20184 ---- Animal proteins ---- Centromere protein H Source.67: DFBPPR20245 ---- Animal proteins ---- 39S ribosomal protein L15, mitochondrial Source.68: DFBPPR20322 ---- Animal proteins ---- GTPase Era, mitochondrial Source.69: DFBPPR20410 ---- Animal proteins ---- Protein AF1q Source.70: DFBPPR20582 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 16 homolog Source.71: DFBPPR20664 ---- Animal proteins ---- General transcription factor IIH subunit 2 Source.72: DFBPPR20764 ---- Animal proteins ---- Phosphatidylinositol-glycan biosynthesis class W protein Source.73: DFBPPR20892 ---- Animal proteins ---- Lysosomal thioesterase PPT2 Source.74: DFBPPR20930 ---- Animal proteins ---- Centromere protein U Source.75: DFBPPR21047 ---- Animal proteins ---- WD repeat-containing protein 48 Source.76: DFBPPR21222 ---- Animal proteins ---- Cytosolic carboxypeptidase 3 Source.77: DFBPPR21316 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit alpha Source.78: DFBPPR21434 ---- Animal proteins ---- Ankyrin repeat and SOCS box protein 15 Source.79: DFBPPR21720 ---- Animal proteins ---- Adipocyte plasma membrane-associated protein Source.80: DFBPPR21794 ---- Animal proteins ---- Ankyrin repeat and SOCS box protein 8 Source.81: DFBPPR21867 ---- Animal proteins ---- Ankyrin repeat and SOCS box protein 5 Source.82: DFBPPR22165 ---- Animal proteins ---- Coiled-coil domain-containing protein 106 Source.83: DFBPPR22571 ---- Animal proteins ---- Gypsy retrotransposon integrase-like protein 1 Source.84: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.85: DFBPPR8845 ---- Animal proteins ---- Glycerol-3-phosphate acyltransferase 1, mitochondrial Source.86: DFBPPR9025 ---- Animal proteins ---- Glutamate decarboxylase 2 Source.87: DFBPPR9123 ---- Animal proteins ---- Eukaryotic translation initiation factor 2 subunit 1 Source.88: DFBPPR9132 ---- Animal proteins ---- Dystrophin Source.89: DFBPPR9209 ---- Animal proteins ---- Cytochrome b-245 light chain Source.90: DFBPPR10143 ---- Animal proteins ---- Lysophospholipid acyltransferase 2 Source.91: DFBPPR10146 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit beta-3 Source.92: DFBPPR10154 ---- Animal proteins ---- Glutathione S-transferase 2 Source.93: DFBPPR10314 ---- Animal proteins ---- Angiotensin-converting enzyme Source.94: DFBPPR10315 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit beta-4 Source.95: DFBPPR10326 ---- Animal proteins ---- Alpha-crystallin A chain Source.96: DFBPPR10363 ---- Animal proteins ---- E3 ubiquitin-protein ligase HACE1 Source.97: DFBPPR10451 ---- Animal proteins ---- Eukaryotic translation initiation factor 2 subunit 1 Source.98: DFBPPR10459 ---- Animal proteins ---- Reelin Source.99: DFBPPR10735 ---- Animal proteins ---- Semaphorin-3E Source.100: DFBPPR10930 ---- Animal proteins ---- WD repeat-containing protein 48 Source.101: DFBPPR10953 ---- Animal proteins ---- DNA repair and recombination protein RAD54-like Source.102: DFBPPR11114 ---- Animal proteins ---- E3 ubiquitin-protein ligase listerin Source.103: DFBPPR11190 ---- Animal proteins ---- Mitochondrial tRNA-specific 2-thiouridylase 1 Source.104: DFBPPR11251 ---- Animal proteins ---- Transcriptional enhancer factor TEF-5 Source.105: DFBPPR11619 ---- Animal proteins ---- Exocyst complex component 8 Source.106: DFBPPR11868 ---- Animal proteins ---- Apoptosis inhibitor 5 Source.107: DFBPPR11974 ---- Animal proteins ---- Adipocyte plasma membrane-associated protein Source.108: DFBPPR12079 ---- Animal proteins ---- Transcription factor SPT20 homolog Source.109: DFBPPR12097 ---- Animal proteins ---- Photoreceptor outer segment membrane glycoprotein 2 Source.110: DFBPPR12134 ---- Animal proteins ---- Coiled-coil domain-containing protein 93 Source.111: DFBPPR12237 ---- Animal proteins ---- Thyroid adenoma-associated protein homolog Source.112: DFBPPR12258 ---- Animal proteins ---- Dimethylaniline monooxygenase [N-oxide-forming] 2 Source.113: DFBPPR12281 ---- Animal proteins ---- Ryanodine receptor 1 Source.114: DFBPPR12641 ---- Animal proteins ---- Cytochrome b-245 light chain Source.115: DFBPPR12751 ---- Animal proteins ---- Glutathione S-transferase Mu 1 Source.116: DFBPPR12794 ---- Animal proteins ---- Chloride channel protein 2 Source.117: DFBPPR13029 ---- Animal proteins ---- Ankyrin repeat and SOCS box protein 5 Source.118: DFBPPR13452 ---- Animal proteins ---- Interleukin-6 Source.119: DFBPPR13577 ---- Animal proteins ---- Interleukin-6 Source.120: DFBPPR14161 ---- Marine protein ---- GTPase Era, mitochondrial Source.121: DFBPPR14199 ---- Marine protein ---- Choline transporter-like protein 2 Source.122: DFBPPR14315 ---- Marine protein ---- Protein CfxQ homolog Source.123: DFBPPR14476 ---- Marine protein ---- Protein CfxQ homolog Source.124: DFBPPR14883 ---- Microorganism protein ---- Guanine nucleotide-binding protein alpha-2 subunit Source.125: DFBPPR14903 ---- Microorganism protein ---- Transcription elongation factor SPT6 Source.126: DFBPPR14911 ---- Microorganism protein ---- Eukaryotic peptide chain release factor GTP-binding subunit Source.127: DFBPPR14966 ---- Microorganism protein ---- PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Source.128: DFBPPR14979 ---- Microorganism protein ---- tRNA N6-adenosine threonylcarbamoyltransferase Source.129: DFBPPR15039 ---- Microorganism protein ---- ATP-dependent RNA helicase SUB2 Source.130: DFBPPR15238 ---- Microorganism protein ---- Pre-mRNA-splicing factor CLF1 Source.131: DFBPPR15278 ---- Microorganism protein ---- Protein arginine N-methyltransferase 2 Source.132: DFBPPR15353 ---- Microorganism protein ---- Pre-mRNA-splicing factor ISY1 Source.133: DFBPPR15398 ---- Microorganism protein ---- Transcription activator of gluconeogenesis ERT1 Source.134: DFBPPR15514 ---- Microorganism protein ---- Nucleotide exchange factor SIL1 Source.135: DFBPPR15570 ---- Microorganism protein ---- Glucose starvation modulator protein 1 Source.136: DFBPPR15783 ---- Microorganism protein ---- Uncharacterized killer plasmid pGKl-2 protein 9 Source.137: DFBPPR7856 ---- Plant protein ---- Maturase K Source.138: DFBPPR7960 ---- Plant protein ---- Vicilin Source.139: DFBPPR8241 ---- Plant protein ---- DNA-directed RNA polymerase subunit beta'
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antiviral activity	Chemically synthesized Leu-Leu-Glu-Tyr showed HIV-1 protease (EC 3.4.23.16) inhibitory activity with an IC₅₀ value of 5100 nM.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptides inhibiting HIV-1 protease were isolated from the hydrolysate of oyster (Crassostrea gigas) proteins prepared with thermolysin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Lee TG, Maruyama S. Isolation of HIV-1 protease-inhibiting peptides from thermolysin hydrolysate of oyster proteins. Biochem Biophys Res Commun. 1998 Dec 30;253(3):604-8. PMID: 9918775 DOI: 10.1006/bbrc.1998.9824
Other literature(s)	N.D
PubDate	1998