Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANPE0008(Antiviral peptide)

DFBP ID	DFBPANPE0008
Peptide sequence	EDLIWK
Type	Native peptide
Peptide/Function name	Antiviral peptide

Function-activity relationship

Main bioactivity	Antiviral activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Asp-Leu-Ile-Trp-Lys

Single-letter amino acid

EDLIWK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
802.9 ± 0.3 Da	802.91 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

4.12 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5833^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	Lactoferrin (LF)
Residue position	f(264-269)
Precursor protein(s) search	Source.1: DFBPPR7658 ---- Milk proteins ---- Lactotransferrin Source.2: DFBPPR7713 ---- Milk proteins ---- Lactotransferrin Source.3: DFBPPR8500 ---- Milk proteins ---- Lactotransferrin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antiviral activity

The peptide was activite against Herpes Simplex Virus Type 1 (HSV-1); Neither peptide EDLIWK (264-269) nor peptide ADRDQYELL (222-230), obtained by chemical synthesis, displayed inhibitory effect when separately tested. The antiviral activity towards HSV-1 was observed when peptide EDLIWK was tested in a 1:1 stoichiometric association with peptide ADRDQYELL, suggesting that the presence of both peptides was required to prevent HSV-1 infection.

Compounds	EC₅₀
Chemically synthesised fragments with amino acid sequences EDLIWK (264-269) and ADRDQYELL (222-230)	850 ug/ml	440 uM

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide was digested with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Siciliano R, Rega B, Marchetti M, Seganti L, Antonini G, Valenti P. Bovine lactoferrin peptidic fragments involved in inhibition of herpes simplex virus type 1 infection. Biochem Biophys Res Commun. 1999 Oct 14;264(1):19-23. PMID: 10527833 DOI: 10.1006/bbrc.1999.1318
Other literature(s)	N.D
PubDate	1999