Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANPE0009(Antiviral peptide)

DFBP ID	DFBPANPE0009
Peptide sequence	IYEG
Type	Native peptide
Peptide/Function name	Antiviral peptide

Function-activity relationship

Main bioactivity	HIV-1 protease-inhibiting activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ile-Tyr-Glu-Gly

Single-letter amino acid

IYEG

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	480.51 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.1750^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Manila clam (Ruditapes philippinarum)
Precursor protein	Manila clam proteins
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1306 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.2: DFBPPR2182 ---- Plant proteins ---- ATP-citrate synthase beta chain protein 1 Source.3: DFBPPR2543 ---- Plant proteins ---- DNA topoisomerase 3-beta Source.4: DFBPPR2751 ---- Plant proteins ---- Actin-7 Source.5: DFBPPR2763 ---- Plant proteins ---- Actin-1 Source.6: DFBPPR2989 ---- Plant proteins ---- Actin-2 Source.7: DFBPPR3087 ---- Plant proteins ---- Actin-3 Source.8: DFBPPR5185 ---- Plant proteins ---- Actin-1 Source.9: DFBPPR5283 ---- Plant proteins ---- Actin-3 Source.10: DFBPPR5841 ---- Plant proteins ---- Actin-1 Source.11: DFBPPR6550 ---- Plant proteins ---- Actin-3 Source.12: DFBPPR6551 ---- Plant proteins ---- Actin-2 Source.13: DFBPPR6552 ---- Plant proteins ---- Actin-1 Source.14: DFBPPR6670 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.15: DFBPPR7117 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.16: DFBPPR16060 ---- Animal proteins ---- Protein kinase C delta type Source.17: DFBPPR16114 ---- Animal proteins ---- Collagen alpha-5(IV) chain Source.18: DFBPPR16188 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.19: DFBPPR16584 ---- Animal proteins ---- Alpha-centractin Source.20: DFBPPR16845 ---- Animal proteins ---- Glutamate dehydrogenase 1, mitochondrial Source.21: DFBPPR16883 ---- Animal proteins ---- Actin, aortic smooth muscle Source.22: DFBPPR16978 ---- Animal proteins ---- Enteropeptidase Source.23: DFBPPR17123 ---- Animal proteins ---- Retinal guanylyl cyclase 2 Source.24: DFBPPR17142 ---- Animal proteins ---- Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Source.25: DFBPPR17154 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.26: DFBPPR17155 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.27: DFBPPR17259 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 35 Source.28: DFBPPR17335 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, liver type Source.29: DFBPPR17430 ---- Animal proteins ---- Short-chain specific acyl-CoA dehydrogenase, mitochondrial Source.30: DFBPPR17514 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.31: DFBPPR17747 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.32: DFBPPR17827 ---- Animal proteins ---- Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial Source.33: DFBPPR17900 ---- Animal proteins ---- Acetyl-CoA carboxylase 1 Source.34: DFBPPR18188 ---- Animal proteins ---- Beta-2-glycoprotein 1 Source.35: DFBPPR18262 ---- Animal proteins ---- Claudin-1 Source.36: DFBPPR18495 ---- Animal proteins ---- Actin, gamma-enteric smooth muscle Source.37: DFBPPR18856 ---- Animal proteins ---- Synaptic vesicle glycoprotein 2A Source.38: DFBPPR20642 ---- Animal proteins ---- Probable ATP-dependent RNA helicase DDX52 Source.39: DFBPPR21096 ---- Animal proteins ---- Kinesin light chain 4 Source.40: DFBPPR21577 ---- Animal proteins ---- Actin-like protein 7A Source.41: DFBPPR22396 ---- Animal proteins ---- Actin-related protein 10 Source.42: DFBPPR22537 ---- Animal proteins ---- Radial spoke head 10 homolog B Source.43: DFBPPR22679 ---- Animal proteins ---- MORN repeat-containing protein 3 Source.44: DFBPPR22697 ---- Animal proteins ---- MORN repeat-containing protein 2 Source.45: DFBPPR22726 ---- Animal proteins ---- Uncharacterized protein C16orf90 homolog Source.46: DFBPPR8560 ---- Animal proteins ---- Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Source.47: DFBPPR8884 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.48: DFBPPR9024 ---- Animal proteins ---- Glutamate dehydrogenase 1, mitochondrial Source.49: DFBPPR9182 ---- Animal proteins ---- Short-chain specific acyl-CoA dehydrogenase, mitochondrial Source.50: DFBPPR10036 ---- Animal proteins ---- Acetyl-CoA carboxylase Source.51: DFBPPR10103 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.52: DFBPPR10319 ---- Animal proteins ---- Actin, alpha cardiac muscle 1 Source.53: DFBPPR10338 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.54: DFBPPR10431 ---- Animal proteins ---- Actin, aortic smooth muscle Source.55: DFBPPR10478 ---- Animal proteins ---- Actin, gamma-enteric smooth muscle Source.56: DFBPPR10777 ---- Animal proteins ---- Actin, cytoplasmic type 5 Source.57: DFBPPR10810 ---- Animal proteins ---- Actin, cytoplasmic 2 Source.58: DFBPPR10844 ---- Animal proteins ---- 60S ribosomal protein L5 Source.59: DFBPPR11013 ---- Animal proteins ---- Band 3 anion transport protein Source.60: DFBPPR12268 ---- Animal proteins ---- Actin, alpha skeletal muscle Source.61: DFBPPR12387 ---- Animal proteins ---- Voltage-dependent calcium channel subunit alpha-2/delta-1 Source.62: DFBPPR12460 ---- Animal proteins ---- ATP-dependent 6-phosphofructokinase, platelet type Source.63: DFBPPR12556 ---- Animal proteins ---- Actin, aortic smooth muscle Source.64: DFBPPR12700 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.65: DFBPPR12701 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.66: DFBPPR12808 ---- Animal proteins ---- UDP-glucuronosyltransferase 1-6 Source.67: DFBPPR12851 ---- Animal proteins ---- UDP-glucuronosyltransferase 1A4 Source.68: DFBPPR13230 ---- Animal proteins ---- Stromelysin-1 Source.69: DFBPPR13231 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.70: DFBPPR13603 ---- Animal proteins ---- Acetyl-CoA carboxylase 1 Source.71: DFBPPR13769 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.72: DFBPPR14021 ---- Animal proteins ---- Actin, cytoplasmic 1 Source.73: DFBPPR14143 ---- Marine protein ---- Actin, cytoplasmic 1 Source.74: DFBPPR15660 ---- Microorganism protein ---- ASTRA-associated protein 1 Source.75: DFBPPR7844 ---- Plant protein ---- Actin-1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antiviral activity	HIV-1 protease-inhibiting activity. Chemically synthesized IYEG showed the IC₅₀ value of 22.3 uM. This peptide was approximately 10-fold more potent as a HIV-1 protease inhibitor than pepstatin A (IC₅₀ = 250 uM), a characteristic inhibitor of aspartic proteases.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)NCC(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Manila clam was digested with thermolysin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Although the peptide purified from thermolysin hydrolysate of manila clam proteins, the origin of this peptide is unclear, it may be come from some microbial proteins in manila clam (such as Physarum polycephalum actin, position: 165-168; Mycobacterium smegmatis ferredoxin, position: 25-28.)

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Lee, T.-G., Yeum, D.-M. Isolation of HIV-1 Protease Inhibiting Peptide from Thermolysin Hydrolysate of Manila Clam Proteins. Preventive Nutrition and Food Science. 2003, 8, 154-7.
Other literature(s)	N.D
PubDate	2003