Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0010(Antithrombotic peptide)

DFBP ID	DFBPANTH0010
Peptide sequence	GPRGP
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Pro-Arg-Gly-Pro

Single-letter amino acid

GPRGP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	482.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.7000^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig
Precursor protein	Porcine skin collagen
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0841 ---- Plant proteins ---- Catalase isozyme A Source.2: DFBPPR1518 ---- Plant proteins ---- GATA transcription factor 15 Source.3: DFBPPR2023 ---- Plant proteins ---- Mitogen-activated protein kinase 9 Source.4: DFBPPR2091 ---- Plant proteins ---- Cellulose synthase-like protein D3 Source.5: DFBPPR2355 ---- Plant proteins ---- Probable glycosyltransferase 5 Source.6: DFBPPR5399 ---- Plant proteins ---- Catalase isozyme 3 Source.7: DFBPPR7125 ---- Plant proteins ---- Catalase isozyme 2 Source.8: DFBPPR8458 ---- Plant proteins ---- Catalase Source.9: DFBPPR15935 ---- Animal proteins ---- Catalase Source.10: DFBPPR16048 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.11: DFBPPR16105 ---- Animal proteins ---- Collagen alpha-1(XVII) chain Source.12: DFBPPR16284 ---- Animal proteins ---- Collagen alpha-2(I) chain Source.13: DFBPPR16842 ---- Animal proteins ---- Collagen alpha-1(II) chain Source.14: DFBPPR16960 ---- Animal proteins ---- Catalase Source.15: DFBPPR17010 ---- Animal proteins ---- Sestrin-2 Source.16: DFBPPR17088 ---- Animal proteins ---- Collagen alpha-2(I) chain Source.17: DFBPPR17360 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.18: DFBPPR17483 ---- Animal proteins ---- Speckle targeted PIP5K1A-regulated poly(A) polymerase Source.19: DFBPPR17613 ---- Animal proteins ---- Collagen alpha-1(XVII) chain Source.20: DFBPPR18216 ---- Animal proteins ---- Collectin-12 Source.21: DFBPPR18252 ---- Animal proteins ---- Collagen alpha-4(IV) chain Source.22: DFBPPR18537 ---- Animal proteins ---- Collagen alpha-1(III) chain Source.23: DFBPPR18673 ---- Animal proteins ---- Isobutyryl-CoA dehydrogenase, mitochondrial Source.24: DFBPPR18818 ---- Animal proteins ---- Protein-tyrosine sulfotransferase 2 Source.25: DFBPPR19033 ---- Animal proteins ---- Collagen alpha-2(XI) chain Source.26: DFBPPR19120 ---- Animal proteins ---- Collagen alpha-1(X) chain Source.27: DFBPPR19357 ---- Animal proteins ---- Collagen alpha-2(IX) chain Source.28: DFBPPR19792 ---- Animal proteins ---- Cleavage stimulation factor subunit 2 Source.29: DFBPPR20122 ---- Animal proteins ---- Mediator of RNA polymerase II transcription subunit 25 Source.30: DFBPPR20428 ---- Animal proteins ---- Rab effector Noc2 Source.31: DFBPPR20593 ---- Animal proteins ---- Pro-interleukin-16 Source.32: DFBPPR21248 ---- Animal proteins ---- 39S ribosomal protein L4, mitochondrial Source.33: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.34: DFBPPR9221 ---- Animal proteins ---- Catalase Source.35: DFBPPR9981 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.36: DFBPPR9985 ---- Animal proteins ---- Collagen alpha-1(II) chain Source.37: DFBPPR10160 ---- Animal proteins ---- Collagen alpha-2(I) chain Source.38: DFBPPR10422 ---- Animal proteins ---- Collagen alpha-1(XII) chain Source.39: DFBPPR10445 ---- Animal proteins ---- Collagen alpha-1(XVII) chain Source.40: DFBPPR10512 ---- Animal proteins ---- Collagen alpha-1(XIV) chain Source.41: DFBPPR10520 ---- Animal proteins ---- Collagen alpha-2(IX) chain Source.42: DFBPPR10603 ---- Animal proteins ---- Collagen alpha-3(VI) chain Source.43: DFBPPR10607 ---- Animal proteins ---- Collagen alpha-2(VI) chain Source.44: DFBPPR10820 ---- Animal proteins ---- Collagen alpha-1(IX) chain Source.45: DFBPPR10825 ---- Animal proteins ---- Collagen alpha-3(IX) chain Source.46: DFBPPR11017 ---- Animal proteins ---- Collectin-12 Source.47: DFBPPR11462 ---- Animal proteins ---- Collagen alpha-1(VIII) chain Source.48: DFBPPR12281 ---- Animal proteins ---- Ryanodine receptor 1 Source.49: DFBPPR12666 ---- Animal proteins ---- Collagen alpha-2(I) chain Source.50: DFBPPR12746 ---- Animal proteins ---- Collagen alpha-4(IV) chain Source.51: DFBPPR12859 ---- Animal proteins ---- Collagen alpha-1(VIII) chain Source.52: DFBPPR14569 ---- Marine protein ---- Collagen alpha-2(I) chain
Link-research Inductive analysis	Link 1: DFBPANTH0066----Pig----Porcine skin collagen

Biological/Functional activity & target protein

Antithrombotic activity

GPRGP inhibited fibrinogen/thrombin clotting to some degree, but did not inhibit the activity of thrombin.

Table 1 Inhibitory effects of collagen-related synthetic peptides on fibrinogen / thrombin clotting
Peptide	IC50 (μM)	References
Gly-Pro-Arg	250	[1]
Gly-Pro-Arg-Pro	110	[1]
Gly-Pro-Arg-Gly	510	-
Gly-Pro-Arg-Gly-Pro	380
Gly-Pro-Arg-Gly-Pro-Ala	260
Gly-Pro-Arg-Gly-Pro-Pro	230
Gly-Pro-Arg-Pro-Pro	50
Gly-Pro-Arg-Gly-Pro-NH₂	40	[2]

Specific target protein(s)

Potential Target Protein(s):
Fibrinogen gamma chain
Fibrinogen alpha chain
Fibrinogen beta chain
Fibroleukin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthetic peptide
Enzyme(s)/starter culture	The peptide was synthesized by the solid-phase method with a 430A Peptide Synthesizer (Applied Biosystems).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Maruyama, S., Nonaka, I., Tanaka, H. Inhibitory effects of enzymatic hydrolysates of collagen and collagen-related synthetic peptides on fibrinogen/thrombin clotting. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1993, 1164, 215-8. DOI: 10.1016/0167-4838(93)90250-U
Other literature(s)	[1] Laudano A P, Doolittle R F. Studies on synthetic peptides that bind to fibrinogen and prevent fibrin polymerization. Structural requirements, number of binding sites, and species differences.[J]. Biochemistry, 1980, 19(5):1013-1019. [2] Kawasaki K, Hirase K, Tsuji T, et al. Amino acids and peptides XII: synthetic peptides related to the N-terminal portion of fibrin alpha-chain and their inhibitory effects on fibrinogen/thrombin clotting.[J]. Thrombosis Research, 1989, 56(6):757-762.
PubDate	1993