Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0015(Antithrombotic peptide)

DFBP ID	DFBPANTH0015
Peptide sequence	DGEA
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Asp-Gly-Glu-Ala

Single-letter amino acid

DGEA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	390.34 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Collagen
Precursor protein	α1-Chain
Residue position	f(435-438)
Precursor protein(s) search	Source.1: DFBPPR0811 ---- Plant proteins ---- Meiosis-specific protein PAIR2 Source.2: DFBPPR1009 ---- Plant proteins ---- SPX domain-containing protein 4 Source.3: DFBPPR1017 ---- Plant proteins ---- Glucosamine 6-phosphate N-acetyltransferase 1 Source.4: DFBPPR1080 ---- Plant proteins ---- Acetylserotonin O-methyltransferase 1 Source.5: DFBPPR1156 ---- Plant proteins ---- Calcium-dependent protein kinase 19 Source.6: DFBPPR1247 ---- Plant proteins ---- Calcium-dependent protein kinase 15 Source.7: DFBPPR1273 ---- Plant proteins ---- Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 Source.8: DFBPPR1282 ---- Plant proteins ---- Calcium-dependent protein kinase 2 Source.9: DFBPPR1306 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.10: DFBPPR1341 ---- Plant proteins ---- Calcium-dependent protein kinase 14 Source.11: DFBPPR1358 ---- Plant proteins ---- Fructose-bisphosphate aldolase, chloroplastic Source.12: DFBPPR1371 ---- Plant proteins ---- DNA (cytosine-5)-methyltransferase DRM2 Source.13: DFBPPR1380 ---- Plant proteins ---- Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 Source.14: DFBPPR1387 ---- Plant proteins ---- Calcium-transporting ATPase 7, plasma membrane-type Source.15: DFBPPR1428 ---- Plant proteins ---- Inositol 3-kinase Source.16: DFBPPR1605 ---- Plant proteins ---- Phytochrome B Source.17: DFBPPR1618 ---- Plant proteins ---- Heat stress transcription factor C-1a Source.18: DFBPPR1707 ---- Plant proteins ---- Probable histidine kinase 2 Source.19: DFBPPR1732 ---- Plant proteins ---- DNA damage-binding protein 2 Source.20: DFBPPR1958 ---- Plant proteins ---- Phospholipase D alpha 2 Source.21: DFBPPR1965 ---- Plant proteins ---- DNA polymerase I B, mitochondrial Source.22: DFBPPR2013 ---- Plant proteins ---- Laccase-6 Source.23: DFBPPR2046 ---- Plant proteins ---- Double-strand break repair protein MRE11 Source.24: DFBPPR2322 ---- Plant proteins ---- Protein ROOT HAIR DEFECTIVE 3 homolog 2 Source.25: DFBPPR2340 ---- Plant proteins ---- Protein NODULATION SIGNALING PATHWAY 1 Source.26: DFBPPR2559 ---- Plant proteins ---- Two-component response regulator ORR27 Source.27: DFBPPR2572 ---- Plant proteins ---- Potassium channel AKT2 Source.28: DFBPPR2617 ---- Plant proteins ---- Clathrin light chain 3 Source.29: DFBPPR2619 ---- Plant proteins ---- Phosphate transporter PHO1-3 Source.30: DFBPPR2804 ---- Plant proteins ---- Protein GIGANTEA Source.31: DFBPPR3011 ---- Plant proteins ---- Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG4 Source.32: DFBPPR3283 ---- Plant proteins ---- Auxin-responsive protein IAA21 Source.33: DFBPPR3386 ---- Plant proteins ---- Auxin-responsive protein IAA2 Source.34: DFBPPR3427 ---- Plant proteins ---- Putrescine hydroxycinnamoyltransferase 1 Source.35: DFBPPR3433 ---- Plant proteins ---- Putrescine hydroxycinnamoyltransferase 3 Source.36: DFBPPR3509 ---- Plant proteins ---- Tryptamine benzoyltransferase 2 Source.37: DFBPPR3559 ---- Plant proteins ---- Putative protein phosphatase 2C 22 Source.38: DFBPPR3587 ---- Plant proteins ---- Auxin transport protein BIG Source.39: DFBPPR3606 ---- Plant proteins ---- COBRA-like protein 7 Source.40: DFBPPR3720 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 36 Source.41: DFBPPR3756 ---- Plant proteins ---- Squamosa promoter-binding-like protein 12 Source.42: DFBPPR3762 ---- Plant proteins ---- Chromatin assembly factor 1 subunit FAS2 homolog Source.43: DFBPPR3884 ---- Plant proteins ---- Casparian strip membrane protein 4 Source.44: DFBPPR4035 ---- Plant proteins ---- Probable transcription factor MYB58 Source.45: DFBPPR4069 ---- Plant proteins ---- Putative magnesium transporter MRS2-D Source.46: DFBPPR4084 ---- Plant proteins ---- Putative serpin-Z8 Source.47: DFBPPR4092 ---- Plant proteins ---- Putative serpin-Z5 Source.48: DFBPPR4426 ---- Plant proteins ---- Protein argonaute 18 Source.49: DFBPPR4661 ---- Plant proteins ---- Protein argonaute 4B Source.50: DFBPPR4710 ---- Plant proteins ---- Ubiquitin-fold modifier-conjugating enzyme 1 Source.51: DFBPPR4763 ---- Plant proteins ---- Cyclin-P2-1 Source.52: DFBPPR4780 ---- Plant proteins ---- Ripening-related protein 3 Source.53: DFBPPR4824 ---- Plant proteins ---- Putative B3 domain-containing protein Os06g0632500 Source.54: DFBPPR4862 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 37 Source.55: DFBPPR4908 ---- Plant proteins ---- Calcium-dependent protein kinase 1 Source.56: DFBPPR4929 ---- Plant proteins ---- DNA polymerase I A, chloroplastic Source.57: DFBPPR5099 ---- Plant proteins ---- Metalloendoproteinase 1 Source.58: DFBPPR5307 ---- Plant proteins ---- 4-coumarate--CoA ligase 1 Source.59: DFBPPR5319 ---- Plant proteins ---- Nodulin-22 Source.60: DFBPPR5496 ---- Plant proteins ---- DIMBOA UDP-glucosyltransferase BX8 Source.61: DFBPPR7417 ---- Plant proteins ---- Cruciferin Source.62: DFBPPR7444 ---- Plant proteins ---- Cruciferin BnC2 Source.63: DFBPPR7446 ---- Plant proteins ---- Cruciferin BnC1 Source.64: DFBPPR8437 ---- Plant proteins ---- Linoleate 9S-lipoxygenase Source.65: DFBPPR16048 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.66: DFBPPR16059 ---- Animal proteins ---- Rho guanine nucleotide exchange factor 2 Source.67: DFBPPR16842 ---- Animal proteins ---- Collagen alpha-1(II) chain Source.68: DFBPPR17011 ---- Animal proteins ---- E3 SUMO-protein ligase RanBP2 Source.69: DFBPPR17195 ---- Animal proteins ---- Receptor for retinol uptake STRA6 Source.70: DFBPPR17360 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.71: DFBPPR17465 ---- Animal proteins ---- 60 kDa heat shock protein, mitochondrial Source.72: DFBPPR17552 ---- Animal proteins ---- Ceramide synthase 4 Source.73: DFBPPR17591 ---- Animal proteins ---- 72 kDa type IV collagenase Source.74: DFBPPR17684 ---- Animal proteins ---- Leukotriene A-4 hydrolase Source.75: DFBPPR17691 ---- Animal proteins ---- Integrin alpha-L Source.76: DFBPPR17805 ---- Animal proteins ---- SNW domain-containing protein 1 Source.77: DFBPPR17854 ---- Animal proteins ---- Tyrosine 3-monooxygenase Source.78: DFBPPR17949 ---- Animal proteins ---- Insulinoma-associated protein 1 Source.79: DFBPPR18236 ---- Animal proteins ---- Latent-transforming growth factor beta-binding protein 2 Source.80: DFBPPR18372 ---- Animal proteins ---- V-type proton ATPase catalytic subunit A Source.81: DFBPPR18757 ---- Animal proteins ---- Mevalonate kinase Source.82: DFBPPR18946 ---- Animal proteins ---- Paternally-expressed gene 3 protein Source.83: DFBPPR19008 ---- Animal proteins ---- GTP-binding protein 1 Source.84: DFBPPR19187 ---- Animal proteins ---- Activating signal cointegrator 1 complex subunit 3 Source.85: DFBPPR19208 ---- Animal proteins ---- Sushi repeat-containing protein SRPX2 Source.86: DFBPPR19254 ---- Animal proteins ---- Periaxin Source.87: DFBPPR19360 ---- Animal proteins ---- KN motif and ankyrin repeat domain-containing protein 2 Source.88: DFBPPR19816 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 72 homolog Source.89: DFBPPR20028 ---- Animal proteins ---- Sine oculis-binding protein homolog Source.90: DFBPPR20394 ---- Animal proteins ---- Actin filament-associated protein 1-like 2 Source.91: DFBPPR21451 ---- Animal proteins ---- Nurim Source.92: DFBPPR21609 ---- Animal proteins ---- Protein PHTF1 Source.93: DFBPPR21835 ---- Animal proteins ---- Protein misato homolog 1 Source.94: DFBPPR21951 ---- Animal proteins ---- Protein ABHD11 Source.95: DFBPPR22219 ---- Animal proteins ---- EF-hand and coiled-coil domain-containing protein 1 Source.96: DFBPPR22337 ---- Animal proteins ---- Uncharacterized protein CLBA1 Source.97: DFBPPR8648 ---- Animal proteins ---- Rho guanine nucleotide exchange factor 2 Source.98: DFBPPR9119 ---- Animal proteins ---- Glycine N-methyltransferase Source.99: DFBPPR9200 ---- Animal proteins ---- V-type proton ATPase catalytic subunit A Source.100: DFBPPR9230 ---- Animal proteins ---- Transcription factor SOX-10 Source.101: DFBPPR9465 ---- Animal proteins ---- Pro-epidermal growth factor Source.102: DFBPPR9838 ---- Animal proteins ---- Transcription factor PU.1 Source.103: DFBPPR9839 ---- Animal proteins ---- Nurim Source.104: DFBPPR9981 ---- Animal proteins ---- Collagen alpha-1(I) chain Source.105: DFBPPR10035 ---- Animal proteins ---- 72 kDa type IV collagenase Source.106: DFBPPR10047 ---- Animal proteins ---- Ovocleidin-116 Source.107: DFBPPR10117 ---- Animal proteins ---- 60 kDa heat shock protein, mitochondrial Source.108: DFBPPR10423 ---- Animal proteins ---- Sortilin-related receptor Source.109: DFBPPR10479 ---- Animal proteins ---- Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Source.110: DFBPPR10967 ---- Animal proteins ---- Interphotoreceptor matrix proteoglycan 2 Source.111: DFBPPR11039 ---- Animal proteins ---- Neurexin-3 Source.112: DFBPPR11234 ---- Animal proteins ---- Bleomycin hydrolase Source.113: DFBPPR11287 ---- Animal proteins ---- Lethal(3)malignant brain tumor-like protein 1 Source.114: DFBPPR11336 ---- Animal proteins ---- Monocarboxylate transporter 3 Source.115: DFBPPR11363 ---- Animal proteins ---- Forkhead box protein D3 Source.116: DFBPPR11536 ---- Animal proteins ---- Putative Polycomb group protein ASXL2 Source.117: DFBPPR11910 ---- Animal proteins ---- 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 Source.118: DFBPPR12212 ---- Animal proteins ---- GTPase-activating Rap/Ran-GAP domain-like protein 3 Source.119: DFBPPR12329 ---- Animal proteins ---- Natriuretic peptides A Source.120: DFBPPR12374 ---- Animal proteins ---- Sortilin-related receptor Source.121: DFBPPR12385 ---- Animal proteins ---- Myosin-11 Source.122: DFBPPR12415 ---- Animal proteins ---- 72 kDa type IV collagenase Source.123: DFBPPR12422 ---- Animal proteins ---- Voltage-dependent R-type calcium channel subunit alpha-1E Source.124: DFBPPR12576 ---- Animal proteins ---- Chloride intracellular channel protein 6 Source.125: DFBPPR12682 ---- Animal proteins ---- Glycine N-methyltransferase Source.126: DFBPPR12959 ---- Animal proteins ---- Keratin, type I cytoskeletal 12 Source.127: DFBPPR13867 ---- Animal proteins ---- Acidic leucine-rich nuclear phosphoprotein 32 family member B Source.128: DFBPPR14107 ---- Marine protein ---- E3 ubiquitin-protein ligase rnf146 Source.129: DFBPPR14291 ---- Marine protein ---- Photosystem II D2 protein Source.130: DFBPPR14703 ---- Marine protein ---- Keratin, type I cytoskeletal 13 Source.131: DFBPPR14808 ---- Marine protein ---- Pseudohemocyanin-1 Source.132: DFBPPR15052 ---- Microorganism protein ---- 5'-3' exoribonuclease 2 Source.133: DFBPPR15086 ---- Microorganism protein ---- Pheromone-processing carboxypeptidase KEX1 Source.134: DFBPPR15812 ---- Microorganism protein ---- ATP synthase subunit beta Source.135: DFBPPR15849 ---- Microorganism protein ---- Exoglucanase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity	The peptide showed antithrombotic activity and inhibited platelet adhesion to collagen type I at 6 mM, but the author found DGEA to be inactive at up to 18 mM.
Specific target protein(s)	Potential Target Protein(s): Integrin beta-3 Integrin alpha-IIb Integrin alpha-2 Platelet glycoprotein IX Platelet glycoprotein Ib alpha chain Platelet glycoprotein Ib beta chain Platelet glycoprotein 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=O)O)C(=O)NCC(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Barnes, M.J., Knight, C.G., Farndale, R.W. The use of collagen-based model peptides to investigate platelet-reactive sequences in collagen. Biopolymers. 1996, 40, 383-97. DOI: 10.1002/(SICI)1097-0282(1996)40:4<383::AID-BIP4>3.0.CO;2-S
Other literature(s)	N.D
PubDate	1996