Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0062(Antithrombotic peptide)

DFBP ID	DFBPANTH0062
Peptide sequence	PHPHLSF
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	Other bioactive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Pro-His-Pro-His-Leu-Ser-Phe

Single-letter amino acid

PHPHLSF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	833.94 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.95 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5429^c

Hydrophilic residue ratio

57.14%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Cow milk protein
Precursor protein	κ-Casein
Residue position	f(99-105)
Precursor protein(s) search	Source.1: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.2: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.3: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.4: DFBPPR8492 ---- Milk proteins ---- Kappa-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Antithrombotic activity

The peptide was inhibitor of the chymosin digestion of cow κ-casein. As shown in table 1.

Table 1 Inhibition of the chymosin digestion of cow κ-casein in the presence of various synthetic peptides derived from κ-casein ^[2]
Sources	Peptide	Inhibition (%) of the amount of cow κ-caseinoglycopeptide liberated when cow κ-casein was digested by chymosin in the presence of synthetic peptides
Sources	Peptide	3 mg peptide	4 mg peptide
Without	－	0	0
From cow κ-casein	PHPHLSF	30	+
	Ac-PHLSF	31	44
	PHLSF	47	94
	Ac-HLSF	45	+
	HLSF	16	32
	Ac-LSF	41	38
	MAIPPK	0	0
	NQDK	0	0
	MAIPPKKNQDK	0	0
	LSFMAIPPK	< 10
From fibrinogen γ-chain	GPRP	0	0

+: Determination not possible as the peptide was not soluble in this concentration.
Ac: Acetyl.

Specific target protein(s)

Specific Target Protein(s):
Chymosin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPINPE0001
[D2]	DFBPMUFU0721

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Fiat AM, Levy-Toledano S, Caen JP, Jollès P. Biologically active peptides of casein and lactotransferrin implicated in platelet function. J Dairy Res. 1989;56(3):351-5. PMID: 2760301 DOI: 10.1017/S002202990002879X
Other literature(s)	[1] Xu, Ruo‐Jun. Bioactive Peptides in Milk and their Biological and Health Implications[J]. Food Reviews International, 1998, 14(1):1-16. [2] Fiat A M, Jollès P. Caseins of various origins and biologically active casein peptides and oligosaccharides: Structural and physiological aspects[J]. Molecular & Cellular Biochemistry, 1989, 87(1):5-30.
PubDate	1989