Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPANTH0063(Antithrombotic peptide)

DFBP ID	DFBPANTH0063
Peptide sequence	HLSF
Type	Native peptide
Peptide/Function name	Antithrombotic peptide

Function-activity relationship

Main bioactivity	Antithrombotic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

His-Leu-Ser-Phe

Single-letter amino acid

HLSF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	502.56 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.6500^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Cow milk protein
Precursor protein	κ-Casein
Residue position	f(102-105)
Precursor protein(s) search	Source.1: DFBPPR1565 ---- Plant proteins ---- Nuclear cap-binding protein subunit 1 Source.2: DFBPPR2227 ---- Plant proteins ---- Cyclin-SDS-like Source.3: DFBPPR2692 ---- Plant proteins ---- FACT complex subunit SSRP1-A Source.4: DFBPPR2999 ---- Plant proteins ---- FACT complex subunit SSRP1-B Source.5: DFBPPR3875 ---- Plant proteins ---- Probable glutamyl endopeptidase, chloroplastic Source.6: DFBPPR5536 ---- Plant proteins ---- FACT complex subunit SSRP1 Source.7: DFBPPR5745 ---- Plant proteins ---- Mitochondrial thiamine diphosphate carrier 1 Source.8: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.9: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.10: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.11: DFBPPR8492 ---- Milk proteins ---- Kappa-casein Source.12: DFBPPR15960 ---- Animal proteins ---- Apolipoprotein A-IV Source.13: DFBPPR16017 ---- Animal proteins ---- Glutamate receptor ionotropic, NMDA 1 Source.14: DFBPPR16607 ---- Animal proteins ---- Taste receptor type 1 member 2 Source.15: DFBPPR16676 ---- Animal proteins ---- Olfactory receptor-like protein OLF2 Source.16: DFBPPR17191 ---- Animal proteins ---- Toll-like receptor 4 Source.17: DFBPPR17713 ---- Animal proteins ---- Urokinase plasminogen activator surface receptor Source.18: DFBPPR17880 ---- Animal proteins ---- Apolipoprotein A-IV Source.19: DFBPPR18713 ---- Animal proteins ---- Arginase-1 Source.20: DFBPPR18921 ---- Animal proteins ---- E3 ubiquitin-protein ligase TRIM11 Source.21: DFBPPR19016 ---- Animal proteins ---- Arginase-2, mitochondrial Source.22: DFBPPR19115 ---- Animal proteins ---- CX3C chemokine receptor 1 Source.23: DFBPPR19168 ---- Animal proteins ---- Endoplasmic reticulum-Golgi intermediate compartment protein 3 Source.24: DFBPPR19193 ---- Animal proteins ---- Transmembrane 9 superfamily member 4 Source.25: DFBPPR19215 ---- Animal proteins ---- Dysferlin Source.26: DFBPPR19862 ---- Animal proteins ---- Splicing factor 3B subunit 3 Source.27: DFBPPR20089 ---- Animal proteins ---- Fascin-2 Source.28: DFBPPR20136 ---- Animal proteins ---- ADP-ribosylation factor GTPase-activating protein 2 Source.29: DFBPPR20236 ---- Animal proteins ---- ADP-ribosylation factor GTPase-activating protein 3 Source.30: DFBPPR20405 ---- Animal proteins ---- Oxygen-regulated protein 1 Source.31: DFBPPR20673 ---- Animal proteins ---- Trafficking protein particle complex subunit 9 Source.32: DFBPPR20968 ---- Animal proteins ---- Ras GTPase-activating protein 3 Source.33: DFBPPR21798 ---- Animal proteins ---- RNA-binding protein 44 Source.34: DFBPPR8710 ---- Animal proteins ---- Leptin receptor Source.35: DFBPPR8813 ---- Animal proteins ---- Apolipoprotein A-IV Source.36: DFBPPR8840 ---- Animal proteins ---- Toll-like receptor 4 Source.37: DFBPPR9194 ---- Animal proteins ---- Arginase-1 Source.38: DFBPPR10344 ---- Animal proteins ---- Centromere protein W Source.39: DFBPPR10351 ---- Animal proteins ---- Protein sidekick-2 Source.40: DFBPPR10390 ---- Animal proteins ---- Protein sidekick-1 Source.41: DFBPPR10411 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit Source.42: DFBPPR10965 ---- Animal proteins ---- Nuclear factor 1 X-type Source.43: DFBPPR12279 ---- Animal proteins ---- Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform Source.44: DFBPPR12378 ---- Animal proteins ---- GDH/6PGL endoplasmic bifunctional protein Source.45: DFBPPR12774 ---- Animal proteins ---- Arginase-2, mitochondrial Source.46: DFBPPR12857 ---- Animal proteins ---- Arginase-1 Source.47: DFBPPR12862 ---- Animal proteins ---- Tumor necrosis factor-inducible gene 6 protein Source.48: DFBPPR12920 ---- Animal proteins ---- Arylamine N-acetyltransferase 2 Source.49: DFBPPR15052 ---- Microorganism protein ---- 5'-3' exoribonuclease 2 Source.50: DFBPPR15156 ---- Microorganism protein ---- Vacuolar protein sorting/targeting protein 10 Source.51: DFBPPR15473 ---- Microorganism protein ---- Rhomboid protein 2 Source.52: DFBPPR15821 ---- Microorganism protein ---- Inosose dehydratase Source.53: DFBPPR7784 ---- Plant protein ---- Phosphoenolpyruvate carboxylase 2 Source.54: DFBPPR7955 ---- Plant protein ---- FACT complex subunit SSRP1
Link-research Inductive analysis	Link 1: DFBPANTH0072----Cow milk protein----κ-Casein

Biological/Functional activity & target protein

Antithrombotic activity

The peptide was inhibitor of the chymosin digestion of cow κ-casein. As shown in table 1.

Table 1 Inhibition of the chymosin digestion of cow κ-casein in the presence of various synthetic peptides derived from κ-casein ^[1]
Sources	Peptide	Inhibition (%) of the amount of cow κ-caseinoglycopeptide liberated when cow κ-casein was digested by chymosin in the presence of synthetic peptides
Sources	Peptide	3 mg peptide	4 mg peptide
Without	－	0	0
From cow κ-casein	PHPHLSF	30	+
	Ac-PHLSF	31	44
	PHLSF	47	94
	Ac-HLSF	45	+
	HLSF	16	32
	Ac-LSF	41	38
	MAIPPK	0	0
	NQDK	0	0
	MAIPPKKNQDK	0	0
	LSFMAIPPK	< 10
From fibrinogen γ-chain	GPRP	0	0

+: Determination not possible as the peptide was not soluble in this concentration.
Ac: Acetyl.

Specific target protein(s)

Specific Target Protein(s):
Chymosin

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Fiat AM, Levy-Toledano S, Caen JP, Jollès P. Biologically active peptides of casein and lactotransferrin implicated in platelet function. J Dairy Res. 1989;56(3):351-5. PMID: 2760301 DOI: 10.1017/S002202990002879X
Other literature(s)	[1] Fiat A M, Jollès P. Caseins of various origins and biologically active casein peptides and oligosaccharides: Structural and physiological aspects[J]. Molecular & Cellular Biochemistry, 1989, 87(1):5-30.
PubDate	1989