DFBP ID - DFBPBBBP0001(Blood-brain barrier peptides)

DFBP ID DFBPBBBP0001 Peptide sequence ELYENKPRRPYIL Type Native peptide Peptide/Function name Blood-brain barrier peptide, Neurotensin

Function-activity relationship

Main bioactivity Penetrate blood-brain barrier Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Glu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu Single-letter amino acid ELYENKPRRPYIL Peptide length 13 Peptide mass Experimental mass Theoretical mass 1674 Da 1690.94 Da c Net charge +3 (pH = 1.9), +1 (pH = 6.5) Isoelectric point (pI) 9.52 c IC50 N.D pIC50 N.D GRAVY -1.3154 c Hydrophilic residue ratio 38.46% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Bovine hypothalamus Precursor protein N.D Residue position N.D Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Penetrate blood-barin barrier This study incidated that Neurotensin (ELYENKPRRPYIL) did not readily cross the blood-brain barrier [1]. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Bitter taste prediction SMILES N.D

Preparation method

Mode of preparation Separation Enzyme(s)/starter culture Neurotensin (pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro- Tyr-Ile-Leu-OH) was isolated by Carraway and Leeman from bovine hypothalamus [2].

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 3323

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Carraway R E , Leeman S E . The amino acid sequence of a hypothalamic peptide, neurotensin[J]. Journal of Biological Chemistry, 1975, 250(5):1907-1911. DOI: 10.1016/S0021-9258(19)41780-8 Other literature(s) [1] Nemeroff CB, Bissette G, Prange AJ Jr, Loosen PT, Barlow TS, Lipton MA. Neurotensin: central nervous system effects of a hypothalamic peptide. Brain Res. 1977 Jun 17;128(3):485-96. doi: 10.1016/0006-8993(77)90173-1. PMID: 406965. [2] Carraway R, Leeman SE. The isolation of a new hypotensive peptide, neurotensin, from bovine hypothalami. J Biol Chem. 1973 Oct 10;248(19):6854-61. PMID: 4745447. PubDate 1975