DFBP ID - DFBPBBBP0002(Blood-brain barrier peptides)

DFBP ID DFBPBBBP0002 Peptide sequence CYFQNCPRG Type Native peptide Peptide/Function name Blood-brain barrier peptide, Vasopressin

Function-activity relationship

Main bioactivity Penetrate blood-brain barrier Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Arg-Gly Single-letter amino acid CYFQNCPRG Peptide length 9 Peptide mass Experimental mass Theoretical mass N.D 1087.25 Da c Net charge 0.00 c Isoelectric point (pI) 8.25 c IC50 N.D pIC50 N.D GRAVY -0.7778 c Hydrophilic residue ratio 33.33% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Bovine hypothalamus Precursor protein N.D Residue position N.D Precursor protein(s) search Source.1: DFBPPR17506 ---- Animal proteins ---- Vasopressin-neurophysin 2-copeptin Source.2: DFBPPR13670 ---- Animal proteins ---- Vasopressin-neurophysin 2-copeptin Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Penetrate blood-barin barrier The Nesfatin-1 is relatively stable in blood for the first 20 min after injection, and crosses the BBB by simple diffusion. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Non-bitter taste prediction SMILES N.D

Preparation method

Mode of preparation Separation Enzyme(s)/starter culture Nesfatin-1 is a peptide corresponding to amino acids 1–82 of the larger protein nucleobindin-2 (NUCB2, or DNA binding/EF-hand/acidic protein NEFA) that shows reduced expression in the paraventricular nucleus of the hypothalamus after fasting, and acts through melanocortin signaling to exert satiety effects in rats [1].

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information The limited penetration under physiological conditions does not limit the pharmacological delivery of this satiety peptide (Nesfatin-1) as a potential therapeutic agent.

Database cross-references

BIOPEP-UWM [D1] 2572

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Pan W, Hsuchou H, Kastin AJ. Nesfatin-1 crosses the blood-brain barrier without saturation. Peptides. 2007 Nov;28(11):2223-8. PMID: 17950952 DOI: 10.1016/j.peptides.2007.09.005 Other literature(s) [1] Oh-I S, Shimizu H, Satoh T, Okada S, Adachi S, Inoue K, Eguchi H, Yamamoto M, Imaki T, Hashimoto K, Tsuchiya T, Monden T, Horiguchi K, Yamada M, Mori M. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature. 2006 Oct 12;443(7112):709-12. doi: 10.1038/nature05162. Epub 2006 Oct 1. PMID: 17036007. PubDate 2007