DFBP ID - DFBPBBBP0005(Blood-brain barrier peptides)

DFBP ID DFBPBBBP0005 Peptide sequence YPSKPDNPGEDAPAEDLARYYSALRHYINLITRQRY Type Native peptide Peptide/Function name Blood-brain barrier peptide, Neuropeptide Y

Function-activity relationship

Main bioactivity Penetrate blood-brain barrier Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr Single-letter amino acid YPSKPDNPGEDAPAEDLARYYSALRHYINLITRQRY Peptide length 36 Peptide mass Experimental mass Theoretical mass N.D 4254.62 Da c Net charge 0.00 c Isoelectric point (pI) 7.58 c IC50 N.D pIC50 N.D GRAVY -1.1417 c Hydrophilic residue ratio 38.89% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Porcine brain Precursor protein N.D Residue position N.D Precursor protein(s) search Source.1: DFBPPR19051 ---- Animal proteins ---- Pro-neuropeptide Y Source.2: DFBPPR8927 ---- Animal proteins ---- Pro-neuropeptide Y Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Penetrate blood-barin barrier The NPY can readily enter the brain from blood by diffusion across the BBB. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools No prediction can be made about the peptide bitterness. prediction SMILES N.D

Preparation method

Mode of preparation Separation (trypsin and thermolysin) Enzyme(s)/starter culture The methanol-soluble peptide fraction (1.8 g), obtained from 400 kg of porcine brain which was first subjected to gel filtration on a Sephadex G-25 column [1].

Stability & Cytotoxicity

Peptide stability Literature report: HPLC showed that most of the 125I-NPY reaching the brain remained intact. EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information Neuropeptide Y has a high degree of sequence homology with peptide YY (70%), the newly isolated porcine intestinal peptide, and pancreatic polypeptide (50%). Therefore, neuropeptide Y, peptide YY, and pancreatic polypeptide are members of a newly recognized peptide family [2].

Database cross-references

BIOPEP-UWM [D1] -

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Kastin AJ, Akerstrom V. Nonsaturable entry of neuropeptide Y into brain. Am J Physiol. 1999 Mar;276(3):E479-82. PMID: 10070013 DOI: 10.1152/ajpendo.1999.276.3.E479 Other literature(s) [1] Tatemoto K, Carlquist M, Mutt V. Neuropeptide Y--a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature. 1982 Apr 15;296(5858):659-60. doi: 10.1038/296659a0. PMID: 6896083. [2] Tatemoto K. Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5485-9. doi: 10.1073/pnas.79.18.5485. PMID: 6957876; PMCID: PMC346928. PubDate 1999