Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPBBBP0008(Blood-brain barrier peptides)

DFBP ID	DFBPBBBP0008
Peptide sequence	YPLG
Type	Native peptide
Peptide/Function name	Blood-brain barrier peptide, Melanocyte-stimulati

Function-activity relationship

Main bioactivity	Penetrate blood-brain barrier
Otheir bioactivity	Opioid activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Leu-Gly

Single-letter amino acid

YPLG

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	448.51 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.1250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine hypothalamus
Precursor protein	N.D
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0384 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR1595 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B Source.7: DFBPPR1602 ---- Plant proteins ---- SNW/SKI-interacting protein A Source.8: DFBPPR1605 ---- Plant proteins ---- Phytochrome B Source.9: DFBPPR1614 ---- Plant proteins ---- Bisdemethoxycurcumin synthase Source.10: DFBPPR1652 ---- Plant proteins ---- Photosystem II D2 protein Source.11: DFBPPR1657 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A Source.12: DFBPPR1662 ---- Plant proteins ---- Probable allantoate deiminase Source.13: DFBPPR3218 ---- Plant proteins ---- Probable lipoxygenase 6 Source.14: DFBPPR3648 ---- Plant proteins ---- Putative lipoxygenase 5 Source.15: DFBPPR5063 ---- Plant proteins ---- Photosystem II D2 protein Source.16: DFBPPR5074 ---- Plant proteins ---- Phytochrome B Source.17: DFBPPR5477 ---- Plant proteins ---- Photosystem II D2 protein Source.18: DFBPPR6232 ---- Plant proteins ---- Photosystem II D2 protein Source.19: DFBPPR6710 ---- Plant proteins ---- Photosystem II D2 protein Source.20: DFBPPR6930 ---- Plant proteins ---- Alpha/beta-gliadin Source.21: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I Source.22: DFBPPR6954 ---- Plant proteins ---- Alpha/beta-gliadin clone PTO-A10 Source.23: DFBPPR7054 ---- Plant proteins ---- Photosystem II D2 protein Source.24: DFBPPR8453 ---- Plant proteins ---- Photosystem II D2 protein Source.25: DFBPPR8503 ---- Milk proteins ---- Lipoprotein lipase Source.26: DFBPPR15942 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B Source.27: DFBPPR16111 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A Source.28: DFBPPR17691 ---- Animal proteins ---- Integrin alpha-L Source.29: DFBPPR18706 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A Source.30: DFBPPR18913 ---- Animal proteins ---- NLR family CARD domain-containing protein 4 Source.31: DFBPPR20109 ---- Animal proteins ---- Ovarian cancer G-protein coupled receptor 1 Source.32: DFBPPR20389 ---- Animal proteins ---- Leucine-rich repeat-containing protein 7 Source.33: DFBPPR20620 ---- Animal proteins ---- GDP-D-glucose phosphorylase 1 Source.34: DFBPPR20684 ---- Animal proteins ---- Doublesex- and mab-3-related transcription factor A2 Source.35: DFBPPR20788 ---- Animal proteins ---- MICOS complex subunit MIC27 Source.36: DFBPPR21506 ---- Animal proteins ---- Origin recognition complex subunit 3 Source.37: DFBPPR9298 ---- Animal proteins ---- Melanocortin receptor 4 Source.38: DFBPPR9884 ---- Animal proteins ---- Cartilage intermediate layer protein 1 Source.39: DFBPPR9947 ---- Animal proteins ---- Hypothalamic tetradecapeptide Source.40: DFBPPR10358 ---- Animal proteins ---- Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase Source.41: DFBPPR11114 ---- Animal proteins ---- E3 ubiquitin-protein ligase listerin Source.42: DFBPPR11200 ---- Animal proteins ---- Homeobox protein HMX1 Source.43: DFBPPR11641 ---- Animal proteins ---- MICOS complex subunit MIC27 Source.44: DFBPPR12041 ---- Animal proteins ---- Paladin Source.45: DFBPPR13568 ---- Animal proteins ---- Lipoprotein lipase Source.46: DFBPPR13733 ---- Animal proteins ---- Keratin-associated protein 8-1 Source.47: DFBPPR13781 ---- Animal proteins ---- Protein-arginine deiminase type-3 Source.48: DFBPPR14291 ---- Marine protein ---- Photosystem II D2 protein Source.49: DFBPPR14338 ---- Marine protein ---- Photosystem II D2 protein Source.50: DFBPPR15487 ---- Microorganism protein ---- Restriction of telomere capping protein 1 Source.51: DFBPPR7775 ---- Plant protein ---- Photosystem II D2 protein Source.52: DFBPPR7802 ---- Plant protein ---- Phytochrome B Source.53: DFBPPR8050 ---- Plant protein ---- Photosystem II D2 protein Source.54: DFBPPR8226 ---- Plant protein ---- Photosystem II D2 protein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Penetrate blood-barin barrier	The Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH₂) is transported across the blood-brain barrier in the direction of brain to blood by a carrier-mediated system referred to as peptide transport system (PTS)-1 ^[1].
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)NCC(=O)O

Preparation method

Mode of preparation	Separation
Enzyme(s)/starter culture	The bovine hypothalamic tissue was heated to inactivate enzymes, homogenized, and centrifuged at high speed to remove large substances. Other proteins were removed in the early fractions obtained from gel filtration of the extract on a Sephadex G-10 column in 0.02 M acetic acid, 0.002% sodium azide.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The Tyr-MIF-1 can enter the brain from the blood to a greater extent than does albumin, but that this penetration does not involve a saturable system ^[2].

Database cross-references

DFBP

[D1]	DFBPOPIO0066
[D2]	DFBPMUFU0762

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Horvath A , Kastin A J . Isolation of tyrosine-melanocyte-stimulating hormone release-inhibiting factor 1 from bovine brain tissue.[J]. Journal of Biological Chemistry, 1989, 264(4):2175-2179. DOI: 10.1016/S0021-9258(18)94157-8
Other literature(s)	[1] Banks WA, Kastin AJ. Modulation of the carrier-mediated transport of the Tyr-MIF-1 across the blood-brain barrier by essential amino acids. J Pharmacol Exp Ther. 1986 Dec;239(3):668-72. PMID: 2879030. [2] Barrera CM, Banks WA, Kastin AJ. Passage of Tyr-MIF-1 from blood to brain. Brain Res Bull. 1989 Dec;23(6):439-42. doi: 10.1016/0361-9230(89)90186-x. PMID: 2575441.
PubDate	1989