Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPBBBP0010(Blood-brain barrier peptides)

DFBP ID	DFBPBBBP0010
Peptide sequence	HSDGIFTDSYSRYRKQMAVKKYLAAVLGKRYKQRVKNK
Type	Native peptide
Peptide/Function name	Blood-brain barrier peptide, Pituitary adenylate

Function-activity relationship

Main bioactivity	Penetrate blood-brain barrier
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys

Single-letter amino acid

HSDGIFTDSYSRYRKQMAVKKYLAAVLGKRYKQRVKNK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	4535.27 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.80 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.0605^c

Hydrophilic residue ratio

34.21%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Ovine hypothalamic tissues
Precursor protein	N.D
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR17237 ---- Animal proteins ---- Pituitary adenylate cyclase-activating polypeptide Source.2: DFBPPR8833 ---- Animal proteins ---- Pituitary adenylate cyclase-activating polypeptide Source.3: DFBPPR13610 ---- Animal proteins ---- Pituitary adenylate cyclase-activating polypeptide
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Penetrate blood-barin barrier	The results show that [¹²⁵I]P38 is able to cross the BBB in both the direction of CNS to blood and blood to brain ^[1].
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Separation
Enzyme(s)/starter culture	N.D

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Based on the result of amino acid analysis, the final yield of the peptide was about 1.2 nmole from 2,400 g of ovine hypothalam.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Miyata A , Arimura A , Dahl R R , et al. Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate cyclase in pituitary cells.[J]. Biochemical & Biophysical Research Communications, 1989, 164(1):567-574. DOI: 10.1016/0006-291x(89)91757-9
Other literature(s)	[1] Banks WA, Kastin AJ, Komaki G, Arimura A. Passage of pituitary adenylate cyclase activating polypeptide1-27 and pituitary adenylate cyclase activating polypeptide1-38 across the blood-brain barrier. J Pharmacol Exp Ther. 1993 Nov;267(2):690-6. PMID: 8246142.
PubDate	1989