Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPCYPE0005(Cytotoxic peptide)

DFBP ID	DFBPCYPE0005
Peptide sequence	IRGCRL
Type	Native peptide
Peptide/Function name	Cytotoxic peptide

Function-activity relationship

Main bioactivity	Cytotoxic peptide activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ile-Arg-Gly-Cys-Arg-Leu

Single-letter amino acid

IRGCRL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
716.6 Da	716.89 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.89 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.2333^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Hen
Precursor protein	Egg-white lysozyme
Residue position	f(124-129)
Precursor protein(s) search	Source.1: DFBPPR9965 ---- Animal proteins ---- Lysozyme C
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Cytotoxic activity

Oxygen radical absorbance capacity-fluorescein (ORAC-FL) assay	The peptide presented antioxidant activity with values of 0.313 ± 0.029 umol Trolox/umol peptide.
Thiobarbituric acid reactive substances (TBARS) in zebrafish larvae	The peptide was found to efficiently inhibit lipid perosidation (61.0 % inhibition of lipid peroxidation).
Toxicity in the Zebrafish model (Danio rerio)	The peptide was not toxic in zebrafish eggs and larvae at concentrations lower than 50 ug/ml. Concentrations higher than 50 ug/ml was toxic for both zebrafish eggs and larvae.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)NCC(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Structure

Embedded Mol* Viewer

Docking

Embedded Mol* Viewer

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Lysozyme was hydrolyzed in situ with pepsin to generate positive charged peptides.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	In the zebrafish larvae test, this peptide does not present toxicity at a concentration of 50 ug/ml for zebrafish eggs. However, concentration higher than 50 ug/ml of peptide was cytotoxic to zebrafish egg after 24 hours of incubation.
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX0527
[D2]	DFBPMUFU0664

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Carrillo, W., Gómez-Ruiz, J.A., Miralles, B., Ramos, M., Barrio, D., Recio, I. Identification of antioxidant peptides of hen egg-white lysozyme and evaluation of inhibition of lipid peroxidation and cytotoxicity in the Zebrafish model. European Food Research and Technology. 2016, 242, 1777-85. DOI: 10.1007/s00217-016-2677-1
Other literature(s)	N.D
PubDate	2016