Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPDPIV0052(DPP IV-inhibitory peptide)

DFBP ID	DFBPDPIV0052
Peptide sequence	ALGGA
Type	Native peptide
Peptide/Function name	DPP-IV inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	DPP-IV inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ala-Leu-Gly-Gly-Ala

Single-letter amino acid

ALGGA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	387.43 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.3200^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig meat, Spanish dry-cured ham
Precursor protein	The water soluble extract of Spanish dry-cured ham
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2287 ---- Plant proteins ---- Dof zinc finger protein 3 Source.2: DFBPPR3444 ---- Plant proteins ---- Vacuolar iron transporter homolog 3 Source.3: DFBPPR3806 ---- Plant proteins ---- LOB domain-containing protein 6 Source.4: DFBPPR3956 ---- Plant proteins ---- Aquaporin SIP1-1 Source.5: DFBPPR4169 ---- Plant proteins ---- Succinate dehydrogenase subunit 6, mitochondrial Source.6: DFBPPR4891 ---- Plant proteins ---- Isoamylase 1, chloroplastic Source.7: DFBPPR5636 ---- Plant proteins ---- Superoxide dismutase [Mn] 3.3, mitochondrial Source.8: DFBPPR5674 ---- Plant proteins ---- Superoxide dismutase [Mn] 3.4, mitochondrial Source.9: DFBPPR5679 ---- Plant proteins ---- Superoxide dismutase [Mn] 3.2, mitochondrial Source.10: DFBPPR6000 ---- Plant proteins ---- Aquaporin SIP1-2 Source.11: DFBPPR6003 ---- Plant proteins ---- Aquaporin SIP1-1 Source.12: DFBPPR6248 ---- Plant proteins ---- Protein TIC110, chloroplastic Source.13: DFBPPR8382 ---- Plant proteins ---- Cationic peroxidase 1 Source.14: DFBPPR16951 ---- Animal proteins ---- Prostaglandin E synthase 2 Source.15: DFBPPR17425 ---- Animal proteins ---- Dynamin-1 Source.16: DFBPPR17557 ---- Animal proteins ---- Tumor necrosis factor receptor superfamily member 1A Source.17: DFBPPR17972 ---- Animal proteins ---- Telomerase reverse transcriptase Source.18: DFBPPR18132 ---- Animal proteins ---- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Source.19: DFBPPR18613 ---- Animal proteins ---- 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial Source.20: DFBPPR20368 ---- Animal proteins ---- Galectin-3-binding protein Source.21: DFBPPR20960 ---- Animal proteins ---- Complexin-1 Source.22: DFBPPR21679 ---- Animal proteins ---- Protein spinster homolog 1 Source.23: DFBPPR9180 ---- Animal proteins ---- Integrin beta-6 Source.24: DFBPPR12432 ---- Animal proteins ---- Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Source.25: DFBPPR13726 ---- Animal proteins ---- Thyroxine 5-deiodinase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

DPP IV-inhibitory activity

The peptide ALGGA showed Dipeptidyl Peptidase IV (EC 3.4.14.5) inhibitory activity at a lower degree, with IC₅₀ value of > 10 mM. As shown in table 1.

Table 1 IC₅₀ (mM) values of Diprotin A (reference inhibitor) and synthetic peptides against DPP-IV activity
Peptides	IC₅₀ (mM)
Diprotin A (IPI)	0.011 ± 0.001
KA	6.27 ± 0.59
AA	9.40 ± 0.10
GP	9.69 ± 0.49
PL	> 10
AAATP	6.47 ± 0.20
AAAAG	8.13 ± 0.48
ALGGA	> 10
LVSGM	> 10
ASGPINFT	No inhibition ^a
DVITGA	No inhibition^a
SAGPN	No inhibition^a

^a Tested up to 20 mM of peptide.

Specific target protein(s)

Specific Target Protein(s):
Dipeptidyl peptidase 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Extracting
Enzyme(s)/starter culture	The peptide was obtained from the water soluble extract of Spanish dry-cured ham.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX0591
[D2]	DFBPMUFU0688

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Gallego M, Aristoy MC, Toldrá F. Dipeptidyl peptidase IV inhibitory peptides generated in Spanish dry-cured ham. Meat Sci. 2014 Feb;96(2 Pt A):757-61. PMID: 24200567 DOI: 10.1016/j.meatsci.2013.09.014
Other literature(s)	[1] Escudero E, Mora L, Fraser P D, et al. Identification of novel antioxidant peptides generated in Spanish dry-cured ham[J]. Food Chemistry, 2013, 138(2–3):1282-1288.
PubDate	2014