Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPDPIV0072(DPP IV-inhibitory peptide)

DFBP ID	DFBPDPIV0072
Peptide sequence	YPFPGPIPN
Type	Native peptide
Peptide/Function name	DPP-IV inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	DPP-IV inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], Opioid activity ^[D3], Multifunctional activity ^[D4]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Phe-Pro-Gly-Pro-Ile-Pro-Asn

Single-letter amino acid

YPFPGPIPN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1001.14 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.4778^c

Hydrophilic residue ratio

77.78%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Gouda-type cheese
Precursor protein	β-Casein
Residue position	f(60-68)
Precursor protein(s) search	Source.1: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

DPP IV-inhibitory activity	The peptide YPFPGPIPN showed some Dipeptidyl Peptidase IV (EC 3.4.14.5) inhibitory activity with the IC₅₀ value of 670 μM.
Specific target protein(s)	Specific Target Protein(s): Dipeptidyl peptidase 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation	Extracting without enzyme
Enzyme(s)/starter culture	The water-soluble extracts of the gouda-type cheeses were prepared without enzyme.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0662, DFBPACEI1470
[D2]	DFBPANHY0575
[D3]	DFBPOPIO0144
[D4]	DFBPMUFU0146

[D5]

[D6]

[D7]

[D8]

Reference(s)

Primary literature	Uenishi, H., Kabuki, T., Seto, Y., Serizawa, A., Nakajima, H. Isolation and identification of casein-derived dipeptidyl-peptidase 4 (DPP-4)-inhibitory peptide LPQNIPPL from gouda-type cheese and its effect on plasma glucose in rats. International Dairy Journal. 2012, 22, 24-30. DOI: 10.1016/j.idairyj.2011.08.002
Other literature(s)	[1] Ojeda M J, Cereto-Massagué A, Valls C, et al. DPP-IV, An Important Target for Antidiabetic Functional Food Design[J]. 2014.
PubDate	2012