Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPDPIV0097(DPP IV-inhibitory peptide)

DFBP ID	DFBPDPIV0097
Peptide sequence	IAVPTGVA
Type	Native peptide
Peptide/Function name	DPP-IV inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	DPP-IV inhibitory activity
Otheir bioactivity	Hypocholesterolemic activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ile-Ala-Val-Pro-Thr-Gly-Val-Ala

Single-letter amino acid

IAVPTGVA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	726.86 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.7250^c

Hydrophilic residue ratio

87.5%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soybean
Precursor protein	Glycinin
Residue position	f(142-149)
Precursor protein(s) search	Source.1: DFBPPR4965 ---- Plant proteins ---- Glycinin G1 Source.2: DFBPPR4973 ---- Plant proteins ---- Glycinin G2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

DPP IV-inhibitory activity	The peptide showed potent inhibitory activity for Dipeptidyl-peptidase IV (EC 3.4.14.5) with the IC₅₀ value of 106 μM.
Specific target protein(s)	Specific Target Protein(s): Dipeptidyl peptidase 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)NCC(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide was obtained from the pepsin digestion.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Soy proteins may be sources of DPP-IV inhibitory peptides potentially useful for the prevention of type 2 diabetes.

Database cross-references

DFBP

[D1]	DFBPHYCP0007
[D2]	DFBPMUFU0727

BIOPEP-UWM

[D3]

9023, 9607, 9681

APD

[D4]

BioPepDB

[D5]

MBPDB

[D6]

Reference(s)

Primary literature	Lammi C, Zanoni C, Arnoldi A, Vistoli G. Peptides Derived from Soy and Lupin Protein as Dipeptidyl-Peptidase IV Inhibitors: In Vitro Biochemical Screening and in Silico Molecular Modeling Study. J Agric Food Chem. 2016 Dec 28;64(51):9601-9606. PMID: 27983830 DOI: 10.1021/acs.jafc.6b04041
Other literature(s)	N.D
PubDate	2016