The peptide ILELA showed some Dipeptidyl Peptidase IV (EC 3.4.14.5) inhibitory activity with the IC50 value of 721.1 ± 46.3 μM and the DPP-IV inhibition at 1000 μM was 59.1 ± 2.8 %. The results compared with other peptides was as shown in table1.
Table 1 DPP-IV inhibitory concentration of camel milk protein derived peptides.
Peptide sequence
| DPP-IV inhibition at 1000 μM (%)
| DPP-IV IC50 (μM)
| Mode of inhibition a
| AMPVQAVLP
| 3.8 ± 2.8
| > 1000
| not determined
| FLQY
| 40.8 ± 5.3
| > 1000
| not determined | FQLGASPY
| 14.1 ± 5.6
| > 1000
| not determined | ILDKEGIDY
| 79.0 ± 6.6
| 347.8 ± 42.8
| mixed-type
| ILDKVGINY b
| 78.8 ± 5.5
| 321.5 ± 29.5
| mixed-type | ILELA
| 59.1 ± 2.8
| 721.1 ± 46.3
| competitive
| ILELAVVSPIQFR
| non measurable
| non measurable
| not determined | LLQLEAIR
| 83.6 ± 4.6
| 177.8 ± 22.2
| competitive | LPVP
| 93.3 ± 3.6
| 87.0 ± 3.2
| competitive | LQALHQGQIV
| 27.2 ± 5.6
| > 1000
| not determined | MPVQA
| 94.0 ± 1.1
| 93.3 ± 8.0
| competitive | VLPVP
| -11.8 ± 3.3
| > 1000
| not determined | SPVVPF
| 88.3 ± 1.5
| 214.1 ± 16.7
| mixed-type | YPVEPF b
| 95.0 ± 3.0
| 138.0 ± 15.3
| mixed-type | a The mode of DPP-IV inhibition was determined using the Lineweaver and Burk double reciprocal representation.
b Homologous peptide from bovine milk proteins.
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