Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPDPIV0213(DPP IV-inhibitory peptide)

DFBP ID	DFBPDPIV0213
Peptide sequence	GLDIQK
Type	Native peptide
Peptide/Function name	DPP-IV inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	DPP-IV inhibitory activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antioxidative activity ^[D2], Hypocholesterolemic activity ^[D3], Multifunctional activity ^[D4]

Calculated physicochemical properties

Three-letter amino acid

Gly-Leu-Asp-Ile-Gln-Lys

Single-letter amino acid

GLDIQK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	672.77 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.71 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk whey protein
Precursor protein	β-Lactoglobulin
Residue position	f(9-14)
Precursor protein(s) search	Source.1: DFBPPR0115 ---- Milk proteins ---- Beta-lactoglobulin Source.2: DFBPPR7687 ---- Milk proteins ---- Beta-lactoglobulin Source.3: DFBPPR7698 ---- Milk proteins ---- Beta-lactoglobulin-1/B Source.4: DFBPPR7714 ---- Milk proteins ---- Beta-lactoglobulin Source.5: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

DPP IV-inhibitory activity	The peptide showed almost no Dipeptidyl-peptidase IV (EC 3.4.14.5) inhibitory activity with an IC₅₀ value of > 1000 μM (No inhibition was observed at highest concentration used in the study 1000 μM).
Specific target protein(s)	Specific Target Protein(s): Dipeptidyl peptidase 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Intact bovine β-lactoglobulin was hydrolyzed with TPCK-trypsin for 24 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

β-Lactoglobulin derived peptides may form part of a functional food based dietary strategy for the management of CVD and T2DM.

Database cross-references

DFBP

[D1]	DFBPACEI0360
[D2]	DFBPANOX0696
[D3]	DFBPHYCP0002
[D4]	DFBPMUFU0110

[D5]

[D6]

[D7]

[D8]

Reference(s)

Primary literature	Power, O., Fernández, A., Norris, R., Riera, F.A., FitzGerald, R.J. Selective enrichment of bioactive properties during ultrafiltration of a tryptic digest of β-lactoglobulin. Journal of Functional Foods. 2014, 9, 38-47. DOI: 10.1016/j.jff.2014.04.002
Other literature(s)	N.D
PubDate	2014