Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPDPIV0217(DPP IV-inhibitory peptide)

DFBP ID	DFBPDPIV0217
Peptide sequence	FPIL
Type	Native peptide
Peptide/Function name	DPP-IV inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	DPP-IV inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Pro-Ile-Leu

Single-letter amino acid

FPIL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
488.68 Da	488.61 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

2.3750^c

Hydrophilic residue ratio

100%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Common Oat (Avena sativa)
Precursor protein	11S globulin
Residue position	f(364–367)
Precursor protein(s) search	Source.1: DFBPPR0860 ---- Plant proteins ---- Soluble starch synthase 3a, chloroplastic/amyloplastic Source.2: DFBPPR1514 ---- Plant proteins ---- DNA polymerase delta catalytic subunit Source.3: DFBPPR1604 ---- Plant proteins ---- Putative bifunctional dihydrofolate reductase-thymidylate synthase Source.4: DFBPPR1623 ---- Plant proteins ---- Probable 4-hydroxy-tetrahydrodipicolinate reductase 2, chloroplastic Source.5: DFBPPR1702 ---- Plant proteins ---- Glutelin type-A 2 Source.6: DFBPPR2035 ---- Plant proteins ---- Glutelin type-A 1 Source.7: DFBPPR2126 ---- Plant proteins ---- Glutelin type-B 2 Source.8: DFBPPR2151 ---- Plant proteins ---- Glutelin type-A 3 Source.9: DFBPPR2386 ---- Plant proteins ---- Probable glucuronosyltransferase Os04g0103100 Source.10: DFBPPR2391 ---- Plant proteins ---- Probable 4-hydroxy-tetrahydrodipicolinate reductase 1, chloroplastic Source.11: DFBPPR2408 ---- Plant proteins ---- Cytochrome f Source.12: DFBPPR2461 ---- Plant proteins ---- Glutelin type-B 1 Source.13: DFBPPR2914 ---- Plant proteins ---- Glutelin type-D 1 Source.14: DFBPPR3128 ---- Plant proteins ---- ABC transporter G family member 41 Source.15: DFBPPR3358 ---- Plant proteins ---- Glutelin type-B 4 Source.16: DFBPPR3666 ---- Plant proteins ---- Glutelin type-B 5 Source.17: DFBPPR3857 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 57 Source.18: DFBPPR4299 ---- Plant proteins ---- Cyclin-J18-like Source.19: DFBPPR4347 ---- Plant proteins ---- Metal transporter Nramp2 Source.20: DFBPPR4479 ---- Plant proteins ---- Metal transporter Nramp6 Source.21: DFBPPR4499 ---- Plant proteins ---- Hydroxycinnamoyltransferase 2 Source.22: DFBPPR5003 ---- Plant proteins ---- Probable glutathione S-transferase Source.23: DFBPPR5075 ---- Plant proteins ---- DNA polymerase delta catalytic subunit Source.24: DFBPPR5137 ---- Plant proteins ---- Cytochrome f Source.25: DFBPPR5256 ---- Plant proteins ---- Early nodulin-70 Source.26: DFBPPR5543 ---- Plant proteins ---- Bifunctional dihydrofolate reductase-thymidylate synthase Source.27: DFBPPR5702 ---- Plant proteins ---- 1-Cys peroxiredoxin PER1 Source.28: DFBPPR5828 ---- Plant proteins ---- Cytochrome f Source.29: DFBPPR6219 ---- Plant proteins ---- Primary amine oxidase Source.30: DFBPPR6392 ---- Plant proteins ---- Cytochrome f Source.31: DFBPPR6805 ---- Plant proteins ---- Cytochrome f Source.32: DFBPPR6888 ---- Plant proteins ---- NADH-ubiquinone oxidoreductase chain 6 Source.33: DFBPPR6936 ---- Plant proteins ---- Alpha/beta-gliadin A-II Source.34: DFBPPR7194 ---- Plant proteins ---- Cytochrome f Source.35: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.36: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.37: DFBPPR8421 ---- Plant proteins ---- 11-beta-hydroxysteroid dehydrogenase B Source.38: DFBPPR16049 ---- Animal proteins ---- Cytochrome P450 1A2 Source.39: DFBPPR16070 ---- Animal proteins ---- Cytochrome P450 1A1 Source.40: DFBPPR16215 ---- Animal proteins ---- Cytochrome P450 2B11 Source.41: DFBPPR16521 ---- Animal proteins ---- Interleukin-3 Source.42: DFBPPR16707 ---- Animal proteins ---- Trefoil factor 2 Source.43: DFBPPR17348 ---- Animal proteins ---- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 Source.44: DFBPPR17357 ---- Animal proteins ---- Bardet-Biedl syndrome 4 protein homolog Source.45: DFBPPR17515 ---- Animal proteins ---- 5'-nucleotidase Source.46: DFBPPR17520 ---- Animal proteins ---- Protein arginine N-methyltransferase 5 Source.47: DFBPPR17527 ---- Animal proteins ---- Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Source.48: DFBPPR17587 ---- Animal proteins ---- Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial Source.49: DFBPPR18424 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 2 Source.50: DFBPPR18860 ---- Animal proteins ---- RAS guanyl-releasing protein 2 Source.51: DFBPPR19130 ---- Animal proteins ---- Voltage-dependent calcium channel gamma-3 subunit Source.52: DFBPPR19661 ---- Animal proteins ---- Golgi pH regulator Source.53: DFBPPR20802 ---- Animal proteins ---- Integrator complex subunit 7 Source.54: DFBPPR21839 ---- Animal proteins ---- tRNA N(3)-methylcytidine methyltransferase METTL2 Source.55: DFBPPR22060 ---- Animal proteins ---- Transmembrane protein 126A Source.56: DFBPPR8732 ---- Animal proteins ---- Dihydropyrimidine dehydrogenase [NADP(+)] Source.57: DFBPPR9083 ---- Animal proteins ---- Leukocyte surface antigen CD47 Source.58: DFBPPR10027 ---- Animal proteins ---- Voltage-dependent L-type calcium channel subunit alpha-1D Source.59: DFBPPR10425 ---- Animal proteins ---- Fanconi anemia group J protein homolog Source.60: DFBPPR11189 ---- Animal proteins ---- Pre-mRNA-splicing factor RBM22 Source.61: DFBPPR11440 ---- Animal proteins ---- Golgi pH regulator Source.62: DFBPPR11576 ---- Animal proteins ---- V-set and immunoglobulin domain-containing protein 1 Source.63: DFBPPR11917 ---- Animal proteins ---- Protein VAC14 homolog Source.64: DFBPPR11965 ---- Animal proteins ---- tRNA N(3)-methylcytidine methyltransferase METTL2 Source.65: DFBPPR12013 ---- Animal proteins ---- Integrator complex subunit 7 Source.66: DFBPPR12254 ---- Animal proteins ---- Cytochrome P450 1A2 Source.67: DFBPPR12421 ---- Animal proteins ---- Arylacetamide deacetylase Source.68: DFBPPR12452 ---- Animal proteins ---- Solute carrier family 15 member 2 Source.69: DFBPPR12547 ---- Animal proteins ---- Cytochrome P450 2C2 Source.70: DFBPPR12660 ---- Animal proteins ---- ATP-binding cassette sub-family C member 9 Source.71: DFBPPR12736 ---- Animal proteins ---- Cytochrome P450 2C1 Source.72: DFBPPR12741 ---- Animal proteins ---- Cytochrome P450 2C14 Source.73: DFBPPR12790 ---- Animal proteins ---- Tumor necrosis factor ligand superfamily member 4 Source.74: DFBPPR12921 ---- Animal proteins ---- Cytochrome P450 2C30 Source.75: DFBPPR12943 ---- Animal proteins ---- Solute carrier family 22 member 8 Source.76: DFBPPR14171 ---- Marine protein ---- Golgi pH regulator Source.77: DFBPPR14367 ---- Marine protein ---- Cytochrome f Source.78: DFBPPR14598 ---- Marine protein ---- Interferon-induced GTP-binding protein Mx2 Source.79: DFBPPR15084 ---- Microorganism protein ---- Mannose-1-phosphate guanyltransferase Source.80: DFBPPR15392 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 16 Source.81: DFBPPR15416 ---- Microorganism protein ---- Protein SOP4 Source.82: DFBPPR7810 ---- Plant protein ---- Cytochrome f Source.83: DFBPPR7949 ---- Plant protein ---- Cytochrome f Source.84: DFBPPR8091 ---- Plant protein ---- Cytochrome f Source.85: DFBPPR8243 ---- Plant protein ---- 11S globulin seed storage protein G3 Source.86: DFBPPR8250 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.87: DFBPPR8259 ---- Plant protein ---- Cytochrome f
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

DPP IV-inhibitory activity	The peptide FPIL was found to inhibit Dipeptidyl-peptidase IV (EC 3.4.14.5) by 13.1 ± 3.2% when tested at a concentration of 1 mg/mL compared with the control. The activity of peptides was compared with that of a sitagliptin inhibitor (positive control). Peptides were assayed at a concentration of 1 mg/mL. Values are the mean of triplicate samples.
Specific target protein(s)	Specific Target Protein(s): Dipeptidyl peptidase 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	DPP-IV inhibition of several synthesised peptides that were identified in silico from the hydrolysis of oat proteins with papain or ficin, which are both proteases.

Stability & Cytotoxicity

Peptide stability

Literature report:

Peptide	Solubility in Water	Resistance to Digestion	Allergenicity Probability
FPIL	Poor	No	Non-allergen

EHP-Tool:

Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Bleakley S, Hayes M, O' Shea N, Gallagher E, Lafarga T. Predicted Release and Analysis of Novel ACE-I, Renin, and DPP-IV Inhibitory Peptides from Common Oat (Avena sativa) Protein Hydrolysates Using in Silico Analysis. Foods. 2017 Dec 4;6(12):108. PMID: 29207547 DOI: 10.3390/foods6120108
Other literature(s)	N.D
PubDate	2017