Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPDPIV0228(DPP IV-inhibitory peptide)

DFBP ID	DFBPDPIV0228
Peptide sequence	PYPYEPYEPYPY
Type	Native peptide
Peptide/Function name	DPP-IV inhibitory peptide, Anti-diabetic peptide

Function-activity relationship

Main bioactivity	DPP-IV inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Pro-Tyr-Pro-Tyr-Glu-Pro-Tyr-Glu-Pro-Tyr-Pro-Tyr

Single-letter amino acid

PYPYEPYEPYPY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1577.69 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.19 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.7917^c

Hydrophilic residue ratio

41.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Yak
Precursor protein	Yak bone hydrolysate
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR17849 ---- Animal proteins ---- Fibromodulin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

DPP IV-inhibitory activity

(1) The peptide PYPYEPYEPYPY exhibited the highest Dipeptidyl-peptidase IV (EC 3.4.14.5) inhibition activities with IC₅₀ value of 189.4 µM among the nine peptides from YBG.

(2) Molecular docking predicted the interaction of peptide PYPYEPYEPYPY with DPP-IV residues TRP62, THR304 and SER462, and the binding between them mainly depends on van der Waals forces and hydrogen bonds.
(3) Glucose tolerance tests were performed on C57 mice orally administered synthesized PYPYEPYEPYPY. The maximal decrement in the blood glucose was 10.28 ± 0.47 mmol/L induced by one-time gavage of PYPYEPYEPYPY at 100 mg/kg.

Specific target protein(s)

Specific Target Protein(s):
Dipeptidyl peptidase 4

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Hemorphins (LVVH7, VVH7, H7, VVH6, VVH5, H5 and H4) were synthesized by Altergen (Illkirch, France).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Bu, F.; Yi, D.; Zhang, H.; Li, Q.; Pan, D.; Dang, Y., Isolation and identification of antioxidant and DPP-IV inhibitory peptide PYPYEPYEPYPY from yak bone hydrolysate. Food Sci. Technol. Res. 2021,27 (3), 441-452. DOI: 10.3136/fstr.27.441
Other literature(s)	N.D
PubDate	2021