DFBP ID - DFBPGAGP0039(γ-Glutamyl peptides)

DFBP ID DFBPGAGP0039 Peptide sequence γ-EF Type Native peptide Peptide/Function name γ-Glutamyl peptide

Function-activity relationship

Main bioactivity Calcium-sensing receptor (CaSR) activation Otheir bioactivity DPP IV-inhibitory activity [D1], Multifunctional activity [D2]

Calculated physicochemical properties

Three-letter amino acid γ-Glu-Phe Single-letter amino acid γ-EF Peptide length 2 Peptide mass Experimental mass Theoretical mass N.D 294.30 Da c Net charge 0.00 c Isoelectric point (pI) 3.34 c IC50 N.D pIC50 N.D GRAVY -0.3500 c Hydrophilic residue ratio 50% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Fermented food (Parmesan Cheese) Precursor protein N.D Residue position N.D Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis Link 1: DFBPGAGP0002----Bacillus amyloliquefaciens (GBA) and Aspergillus oryzae (GAO)----N.D Link 2: DFBPGAGP0003----Soybean (Glycine max (L.) Merr.) ----N.D Link 3: DFBPGAGP0014----Fermented food (cheese)----N.D Link 4: DFBPGAGP0016----Fermented food (Soy sauce)----N.D Link 5: DFBPGAGP0041----Fermented food (Sufu)----N.D Link 6: DFBPGAGP0046----Fermented food (Sourdough Fermentation)----N.D Link 7: DFBPGAGP0050----Synthesis peptide----Synthesis peptide

Biological/Functional activity & target protein

Calcium-sensing receptor (CaSR) activation N.D Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report The γ-Glu-Phe exhibited threshold concentration for an unspecific, slightly astringent mouth coating at 2.50 mmol/L. Bitter prediction tools Bitter taste prediction SMILES N.D

Preparation method

Mode of preparation Fermentation Enzyme(s)/starter culture N.D

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information The Contents of γ-Glu-Phe in Parmesan Cheese were 1.146 mmol/L.

Database cross-references

DFBP [D1] DFBPDPIV0243 [D2] DFBPMUFU0740

BIOPEP-UWM [D3] -

APD [D4] -

BioPepDB [D5] -

MBPDB [D6] -

Reference(s)

Primary literature Hillmann H, Hofmann T. Quantitation of Key Tastants and Re-engineering the Taste of Parmesan Cheese. J Agric Food Chem. 2016 Mar 2;64(8):1794-805. PMID: 26870875 DOI: 10.1021/acs.jafc.6b00112 Other literature(s) N.D PubDate 2016