DFBP ID - DFBPHAPE0009(Haemolytic peptide)

DFBP ID DFBPHAPE0009 Peptide sequence FLPLLFGALSAILPKIF Type Native peptide Peptide/Function name Haemolytic peptide

Function-activity relationship

Main bioactivity Haemolytic activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Phe-Leu-Pro-Leu-Leu-Phe-Gly-Ala-Leu-Ser-Ala-Ile-Leu-Pro-Lys-Ile-Phe Single-letter amino acid FLPLLFGALSAILPKIF Peptide length 18 Peptide mass Experimental mass Theoretical mass N.D 1860.31 Da c Net charge 0.00 c Isoelectric point (pI) 10.04 c IC50 N.D pIC50 N.D GRAVY 1.8647 c Hydrophilic residue ratio 88.24% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Frog Rana esculenta Precursor protein Vespa-like peptide from R. Erythraea Residue position N.D Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Haemolytic activity The peptide showed haemolytic activity. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Bitter taste prediction SMILES N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture Synthesis peptide

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 3868

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Simmaco M, De Biase D, Severini C, Aita M, Erspamer GF, Barra D, Bossa F. Purification and characterization of bioactive peptides from skin extracts of Rana esculenta. Biochim Biophys Acta. 1990 Mar 26;1033(3):318-23. PMID: 2317508 DOI: 10.1016/0304-4165(90)90140-r Other literature(s) N.D PubDate 1990