DFBP ID - DFBPHAPE0018(Haemolytic peptide)

DFBP ID DFBPHAPE0018 Peptide sequence LKKISQRYQKFALPQYLKT Type Native peptide Peptide/Function name Haemolytic peptide

Function-activity relationship

Main bioactivity Haemolytic activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Leu-Lys-Lys-Ile-Ser-Gln-Arg-Tyr-Gln-Lys-Phe-Ala-Leu-Pro-Gln-Tyr-Leu-Lys-Thr Single-letter amino acid LKKISQRYQKFALPQYLKT Peptide length 19 Peptide mass Experimental mass Theoretical mass N.D 2353.82 Da c Net charge 0.00 c Isoelectric point (pI) 10.71 c IC50 N.D pIC50 N.D GRAVY -0.8316 c Hydrophilic residue ratio 36.84% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Bovine Precursor protein αs2-Casein Residue position N.D Precursor protein(s) search Source.1: DFBPPR8494 ---- Milk proteins ---- Alpha-S2-casein Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Haemolytic activity The peptide showed haemolytic activity. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Bitter taste prediction SMILES N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)O

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture Synthesis peptide

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 3963

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Recio I, Visser S. Identification of two distinct antibacterial domains within the sequence of bovine alpha(s2)-casein. Biochim Biophys Acta. 1999 Aug 5;1428(2-3):314-26. PMID: 10434050 DOI: 10.1016/s0304-4165(99)00079-3 Other literature(s) N.D PubDate 1999