Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPHEBP0036(Heparin-binding peptide)

DFBP ID	DFBPHEBP0036
Peptide sequence	FKCRRWQWRMKKLGAPSITCVRRAFA
Type	Native peptide
Peptide/Function name	Heparin-binding peptide

Function-activity relationship

Main bioactivity	Heparin-binding activity
Otheir bioactivity	Antimicrobial activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Phe-Lys-Cys-Arg-Arg-Trp-Gln-Trp-Arg-Met-Lys-Lys-Leu-Gly-Ala-Pro-Ser-Ile-Thr-Cys-Val-Arg-Arg-Ala-Phe-Ala

Single-letter amino acid

FKCRRWQWRMKKLGAPSITCVRRAFA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
3195.5 Da	3196.88 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

12.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.4846^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	Lactoferrin
Residue position	f(17-42)
Precursor protein(s) search	Source.1: DFBPPR8500 ---- Milk proteins ---- Lactotransferrin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Heparin-binding activity

This peptide showed potently heparin-binding activity. When this peptide was mixed with heparin, a distinct spectral change was induced because of conformational alteration of the peptide. This spectral change was reversible. Analysis of data from peptide synthesis indicated that binding by the sequence Arg²⁸-Met²⁹-Lys¹⁹-Lys³¹ of bovine lactoferrin is significant and that there is a synergistic contribution from Lys¹⁸-Cys¹⁹-Arg²⁰-Arg²⁰, and Arg³⁸-Arg³⁹.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(C)C)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Bovine lactoferrin hydrolysate was prepared by pepsin digestion.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The heparin-binding site of bovine lactoferrin is proposed to be the sequence BXBB at positions 18–21 and 25–28 and BB at 38–39. The heparin-binding peptide that was isolated from bovine lactoferrin altered its conformation upon interaction with heparin.
This amino acid sequence is the same as that of bovine lactoferricin (LFcin B), except that an Ala residue is attached at its carboxyl end.

Database cross-references

DFBP

[D1]	DFBPAMIC0287
[D2]	DFBPMUFU0708

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Shimazaki K, Tazume T, Uji K, Tanaka M, Kumura H, Mikawa K, Shimo-Oka T. Properties of a heparin-binding peptide derived from bovine lactoferrin. J Dairy Sci. 1998 Nov;81(11):2841-9. PMID: 9839225 DOI: 10.3168/jds.S0022-0302(98)75843-6
Other literature(s)	N.D
PubDate	1998