Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPHYCP0007(Hypocholesterolemic peptide)

DFBP ID	DFBPHYCP0007
Peptide sequence	IAVPTGVA
Type	Native peptide
Peptide/Function name	Hypocholesterolemic peptide

Function-activity relationship

Main bioactivity	Hypocholesterolemic activity
Otheir bioactivity	DPP IV-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ile-Ala-Val-Pro-Thr-Gly-Val-Ala

Single-letter amino acid

IAVPTGVA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	726.86 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.7250^c

Hydrophilic residue ratio

87.5%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soy protein
Precursor protein	11S globulin-derived peptide
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR4965 ---- Plant proteins ---- Glycinin G1 Source.2: DFBPPR4973 ---- Plant proteins ---- Glycinin G2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Hypocholesterolemic activity	An in vitro test for measuring 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC 1.1.1.88) inhibition showed that the synthesized peptide IAVPTGVA spossessed hypocholesterinemic properties. According to the results, the IC₅₀ for IAVPTGVA was measured as 93.3 μM.
Specific target protein(s)	Specific Target Protein(s): 3-hydroxy-3-methylglutaryl coenzyme A reductase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)NCC(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Synthetic peptide
Enzyme(s)/starter culture	The peptide IAVPTGVA was synthetic and retained the IAVP fragment.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPDPIV0097
[D2]	DFBPMUFU0727

BIOPEP-UWM

[D3]

9023, 9607, 9681

APD

[D4]

BioPepDB

[D5]

MBPDB

[D6]

Reference(s)

Primary literature	Pak, V.V., Koo, M., Lee, N., Kim, M.S., Kwon, D.Y. Structure—Activity Relationships of the Peptide Ile-Ala-Val-Pro and Its Derivatives Revealed Using the Semi-Empirical AM1 Method. Chemistry of Natural Compounds. 2005, 41, 454-60. DOI: 10.1007/s10600-005-0176-x
Other literature(s)	N.D
PubDate	2005