Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPHYCP0015(Hypocholesterolemic peptide)

DFBP ID	DFBPHYCP0015
Peptide sequence	YVVNPDNNEN
Type	Native peptide
Peptide/Function name	Hypocholesterolemic peptide

Function-activity relationship

Main bioactivity	Hypocholesterolemic activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Val-Val-Asn-Pro-Asp-Asn-Asn-Glu-Asn

Single-letter amino acid

YVVNPDNNEN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1177.19 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.5500^c

Hydrophilic residue ratio

30%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soybean
Precursor protein	β‐Conglycinin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR5025 ---- Plant proteins ---- Beta-conglycinin alpha subunit 1 Source.2: DFBPPR5036 ---- Plant proteins ---- Beta-conglycinin alpha subunit 2
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Hypocholesterolemic activity

Research carried out in silico on peptide YVVNPDNNEN interaction with the catalytic site of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCoAR, EC 1.1.1.88) demonstrated that it behave as competitive inhibitor of HMGCoAR activity with a statin-like mechanism, confirmed by direct inhibition experiments.
Research in HepG2 cells aimed at investigating the effects on cholesterol metabolism showed that compared to mock treatment peptide YVVNPDNNEN at 350 μM up-regulates the mature SREBP2 protein level by 158.0 ± 9.2%, increases the LDL level 164.0 ± 17.9%, and induces a HMGCoAR protein increase by 170 ± 50.0%.

Specific target protein(s)

Specific Target Protein(s):
3-hydroxy-3-methylglutaryl coenzyme A reductase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation	Synthetic peptide
Enzyme(s)/starter culture	The peptide YVVNPDNNEN was bought from PRIMM (Milan, Italy), which certified > 95% purity by HPLC.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Lammi C, Zanoni C, Arnoldi A, Vistoli G. Two Peptides from Soy β-Conglycinin Induce a Hypocholesterolemic Effect in HepG2 Cells by a Statin-Like Mechanism: Comparative in Vitro and in Silico Modeling Studies. J Agric Food Chem. 2015 Sep 16;63(36):7945-51. PMID: 26310992 DOI: 10.1021/acs.jafc.5b03497
Other literature(s)	N.D
PubDate	2015