Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPICPE0005(Ileum contracting peptide)

DFBP ID	DFBPICPE0005
Peptide sequence	WWGKKYRASKLGLAR
Type	Native peptide
Peptide/Function name	Ileum contracting peptide, Smooth muscles contracting peptide

Function-activity relationship

Main bioactivity	Ileum-contracting activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Trp-Trp-Gly-Lys-Lys-Tyr-Arg-Ala-Ser-Lys-Leu-Gly-Leu-Ala-Arg

Single-letter amino acid

WWGKKYRASKLGLAR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1820.16 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.64 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9467^c

Hydrophilic residue ratio

53.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine
Precursor protein	κ-Casein
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Ileum contracting activity	The peptide WWGKKYRASKLGLAR evokes contraction of the ileal strips.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)NCC(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(C)C)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Peptides were synthesized by the Fmoc strategy using a Sam 2 peptide synthesizer (Biosearch, Inc.). The peptides were purified by reversed-phase HPLC on an octadecyl silica column.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Takahashi M, Moriguchi S, Suganuma H, Shiota A, Tani F, Usui H, Kurahashi K, Sasaki R, Yoshikawa M. Identification of casoxin C, an ileum-contracting peptide derived from bovine kappa-casein, as an agonist for C3a receptors. Peptides. 1997;18(3):329-36. PMID: 9145417 DOI: 10.1016/s0196-9781(97)00044-2
Other literature(s)	N.D
PubDate	1997