DFBP ID - DFBPICPE0007(Ileum contracting peptide)

DFBP ID DFBPICPE0007 Peptide sequence YPMYPLPR Type Native peptide Peptide/Function name Ileum contracting peptide

Function-activity relationship

Main bioactivity Ileum-contracting activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Tyr-Pro-Met-Tyr-Pro-Leu-Pro-Arg Single-letter amino acid YPMYPLPR Peptide length 8 Peptide mass Experimental mass Theoretical mass N.D 1036.24 Da c Net charge 0.00 c Isoelectric point (pI) 9.59 c IC50 N.D pIC50 N.D GRAVY -0.7750 c Hydrophilic residue ratio 62.5% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Plant Organism/Source Rice Precursor protein Rice albumin Residue position N.D Precursor protein(s) search Source.1: DFBPPR3414 ---- Plant proteins ---- Seed allergenic protein RA5 Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Ileum contracting activity The peptide YPMYPLPR exhibits stimulatory activity on ileal contraction. Sequence Rapid Slow EC50 ± SE (μM) n EC50 ± SE (μM) n C3a (70-77) ASHLGLAR 16 ± 5.1 4 1.6 ± 0.40 4 Oryzatensin GYPMYPLPR 4.5 ± 1.6 3 0.082 ± 0.019 4 YPMYPLPR 3.7 ± 1.2 3 0.055 ± 0.017 3 PMYPLPR 18 ± 4.5 3 0.76 ± 0.11 4 MYPLPR 33 ± 13 3 1.2 ± 0.19 3 YPLPR 50 ± 3.3 3 2.2 ± 0.82 3 PLPR * 2 59 ± 15 3 YPMYPLP * 2 * 2 The numbers of guinea pigs used are indicated by n. * No contraction was observed at 1 mM. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Bitter taste prediction SMILES N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture Peptides used in this study were synthesized by the Fmoc strategy using a Sam 2 peptide synthesizer (Biosearch Inc.). The peptides were purified by reversed-phase HPLC on an octadecyl silica column.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 3814

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Takahashi M, Moriguchi S, Ikeno M, Kono S, Ohata K, Usui H, Kurahashi K, Sasaki R, Yoshikawa M. Studies on the ileum-contracting mechanisms and identification as a complement C3a receptor agonist of oryzatensin, a bioactive peptide derived from rice albumin. Peptides. 1996;17(1):5-12. PMID: 8822503 DOI: 10.1016/0196-9781(95)02059-4 Other literature(s) N.D PubDate 1996