Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPICPE0008(Ileum contracting peptide)

DFBP ID	DFBPICPE0008
Peptide sequence	MYPLPR
Type	Native peptide
Peptide/Function name	Ileum contracting peptide

Function-activity relationship

Main bioactivity	Ileum-contracting activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Met-Tyr-Pro-Leu-Pro-Arg

Single-letter amino acid

MYPLPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	775.95 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.82 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.5500^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Rice
Precursor protein	Rice albumin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR3414 ---- Plant proteins ---- Seed allergenic protein RA5
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Ileum contracting activity

The peptide MYPLPR exhibits stimulatory activity on ileal contraction.

Sequence		Rapid		Slow
Sequence		EC₅₀ ± SE (μM)	n	EC₅₀ ± SE (μM)	n
C3a (70-77)	ASHLGLAR	16 ± 5.1	4	1.6 ± 0.40	4
Oryzatensin	GYPMYPLPR	4.5 ± 1.6	3	0.082 ± 0.019	4
	YPMYPLPR	3.7 ± 1.2	3	0.055 ± 0.017	3
	PMYPLPR	18 ± 4.5	3	0.76 ± 0.11	4
	MYPLPR	33 ± 13	3	1.2 ± 0.19	3
	YPLPR	50 ± 3.3	3	2.2 ± 0.82	3
	PLPR	*	2	59 ± 15	3
	YPMYPLP	*	2	*	2
The numbers of guinea pigs used are indicated by n. * No contraction was observed at 1 mM.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Peptides used in this study were synthesized by the Fmoc strategy using a Sam 2 peptide synthesizer (Biosearch Inc.). The peptides were purified by reversed-phase HPLC on an octadecyl silica column.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Takahashi M, Moriguchi S, Ikeno M, Kono S, Ohata K, Usui H, Kurahashi K, Sasaki R, Yoshikawa M. Studies on the ileum-contracting mechanisms and identification as a complement C3a receptor agonist of oryzatensin, a bioactive peptide derived from rice albumin. Peptides. 1996;17(1):5-12. PMID: 8822503 DOI: 10.1016/0196-9781(95)02059-4
Other literature(s)	N.D
PubDate	1996