Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPICPE0009(Ileum contracting peptide)

DFBP ID	DFBPICPE0009
Peptide sequence	PLPR
Type	Native peptide
Peptide/Function name	Ileum contracting peptide

Function-activity relationship

Main bioactivity	Ileum-contracting activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Pro-Leu-Pro-Arg

Single-letter amino acid

PLPR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	481.58 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9750^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Rice
Precursor protein	Rice albumin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0951 ---- Plant proteins ---- Calpain-type cysteine protease ADL1 Source.2: DFBPPR0969 ---- Plant proteins ---- Polyamine oxidase 1 Source.3: DFBPPR0978 ---- Plant proteins ---- Transcription factor MYBS1 Source.4: DFBPPR0989 ---- Plant proteins ---- Calcium-dependent protein kinase 21 Source.5: DFBPPR0990 ---- Plant proteins ---- LysM domain receptor-like kinase 10 Source.6: DFBPPR1125 ---- Plant proteins ---- Protein FLOURY ENDOSPERM 6, chloroplastic Source.7: DFBPPR1314 ---- Plant proteins ---- Ferredoxin-dependent glutamate synthase, chloroplastic Source.8: DFBPPR1336 ---- Plant proteins ---- Protein argonaute 7 Source.9: DFBPPR1481 ---- Plant proteins ---- Leucine-rich repeat receptor protein kinase MSL1 Source.10: DFBPPR1484 ---- Plant proteins ---- Allene oxide synthase 2 Source.11: DFBPPR1501 ---- Plant proteins ---- Polygalacturonase inhibitor 1 Source.12: DFBPPR1613 ---- Plant proteins ---- Villin-3 Source.13: DFBPPR1759 ---- Plant proteins ---- Endoribonuclease Dicer homolog 2a Source.14: DFBPPR1772 ---- Plant proteins ---- Endoribonuclease Dicer homolog 2b Source.15: DFBPPR1805 ---- Plant proteins ---- Probable polyribonucleotide nucleotidyltransferase 1, chloroplastic Source.16: DFBPPR1832 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 7, chloroplastic Source.17: DFBPPR1866 ---- Plant proteins ---- Probable DNA gyrase subunit A, chloroplastic/mitochondrial Source.18: DFBPPR1927 ---- Plant proteins ---- Cyclin-dependent kinase G-1 Source.19: DFBPPR2011 ---- Plant proteins ---- Lipoyl synthase 1, chloroplastic Source.20: DFBPPR2053 ---- Plant proteins ---- Putative laccase-5 Source.21: DFBPPR2141 ---- Plant proteins ---- Beta-amylase 1, chloroplastic Source.22: DFBPPR2321 ---- Plant proteins ---- Aspartate carbamoyltransferase, chloroplastic Source.23: DFBPPR2373 ---- Plant proteins ---- Adagio-like protein 3 Source.24: DFBPPR2386 ---- Plant proteins ---- Probable glucuronosyltransferase Os04g0103100 Source.25: DFBPPR2437 ---- Plant proteins ---- Sialyltransferase-like protein 1 Source.26: DFBPPR2490 ---- Plant proteins ---- Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic Source.27: DFBPPR2576 ---- Plant proteins ---- Probable glycosyltransferase 4 Source.28: DFBPPR2583 ---- Plant proteins ---- Thioredoxin-like 2, chloroplastic Source.29: DFBPPR2609 ---- Plant proteins ---- Auxin response factor 15 Source.30: DFBPPR2779 ---- Plant proteins ---- Auxin response factor 2 Source.31: DFBPPR3155 ---- Plant proteins ---- Acyl transferase 1 Source.32: DFBPPR3277 ---- Plant proteins ---- Alpha-amylase/trypsin inhibitor RA16 Source.33: DFBPPR3304 ---- Plant proteins ---- Probable indole-3-acetic acid-amido synthetase GH3.2 Source.34: DFBPPR3371 ---- Plant proteins ---- Kinesin-like protein KIN-14R Source.35: DFBPPR3414 ---- Plant proteins ---- Seed allergenic protein RA5 Source.36: DFBPPR3427 ---- Plant proteins ---- Putrescine hydroxycinnamoyltransferase 1 Source.37: DFBPPR3437 ---- Plant proteins ---- Putative acetyl-coenzyme A carboxylase carboxyl transferase subunit beta-like protein Source.38: DFBPPR3609 ---- Plant proteins ---- Thioredoxin-like 1-1, chloroplastic Source.39: DFBPPR3889 ---- Plant proteins ---- Lysine-specific histone demethylase 1 homolog 2 Source.40: DFBPPR4093 ---- Plant proteins ---- Villin-5 Source.41: DFBPPR4100 ---- Plant proteins ---- Glycosyltransferase family 92 protein Os08g0121900 Source.42: DFBPPR4120 ---- Plant proteins ---- Probable aquaporin TIP4-3 Source.43: DFBPPR4232 ---- Plant proteins ---- Formin-like protein 14 Source.44: DFBPPR4272 ---- Plant proteins ---- CASP-like protein 3A1 Source.45: DFBPPR4394 ---- Plant proteins ---- Formin-like protein 9 Source.46: DFBPPR4419 ---- Plant proteins ---- Formin-like protein 15 Source.47: DFBPPR4469 ---- Plant proteins ---- Protein argonaute 11 Source.48: DFBPPR4472 ---- Plant proteins ---- BURP domain-containing protein 15 Source.49: DFBPPR4513 ---- Plant proteins ---- Protein SMAX1-like Source.50: DFBPPR4519 ---- Plant proteins ---- Metal transporter Nramp5 Source.51: DFBPPR4633 ---- Plant proteins ---- B3 domain-containing protein Os07g0183700 Source.52: DFBPPR4696 ---- Plant proteins ---- Uncharacterized protein ycf72 Source.53: DFBPPR5046 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 2 Source.54: DFBPPR5049 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 1 Source.55: DFBPPR5231 ---- Plant proteins ---- Casparian strip membrane protein 5 Source.56: DFBPPR5397 ---- Plant proteins ---- Alpha-terpineol synthase, chloroplastic Source.57: DFBPPR5415 ---- Plant proteins ---- Eudesmanediol synthase Source.58: DFBPPR5438 ---- Plant proteins ---- Tau-cadinol synthase Source.59: DFBPPR5454 ---- Plant proteins ---- Ferredoxin-dependent glutamate synthase, chloroplastic Source.60: DFBPPR5464 ---- Plant proteins ---- Alpha-copaene synthase Source.61: DFBPPR5519 ---- Plant proteins ---- Beta-selinene synthase Source.62: DFBPPR5634 ---- Plant proteins ---- Eudesmanediol synthase Source.63: DFBPPR5917 ---- Plant proteins ---- Aquaporin TIP4-4 Source.64: DFBPPR6019 ---- Plant proteins ---- Inactive beta selinene synthase Source.65: DFBPPR6140 ---- Plant proteins ---- Uncharacterized protein ycf72 Source.66: DFBPPR6247 ---- Plant proteins ---- DNA topoisomerase 2 Source.67: DFBPPR6343 ---- Plant proteins ---- Aspartate carbamoyltransferase 3, chloroplastic Source.68: DFBPPR6344 ---- Plant proteins ---- Aspartate carbamoyltransferase 2, chloroplastic Source.69: DFBPPR7039 ---- Plant proteins ---- Nitrate reductase [NAD(P)H] Source.70: DFBPPR7043 ---- Plant proteins ---- Beta-amylase Source.71: DFBPPR7430 ---- Plant proteins ---- Nitrate reductase [NADH], clone PBNBR1412 Source.72: DFBPPR7432 ---- Plant proteins ---- Nitrate reductase [NADH], clone PBNBR1405 Source.73: DFBPPR7523 ---- Plant proteins ---- Non-specific lipid-transfer protein 1 Source.74: DFBPPR7525 ---- Plant proteins ---- Non-specific lipid-transfer protein 2 Source.75: DFBPPR7604 ---- Milk proteins ---- Zinc transporter 2 Source.76: DFBPPR8392 ---- Plant proteins ---- 11-beta-hydroxysteroid dehydrogenase B Source.77: DFBPPR8421 ---- Plant proteins ---- 11-beta-hydroxysteroid dehydrogenase B Source.78: DFBPPR8432 ---- Plant proteins ---- Omega-3 fatty acid desaturase, chloroplastic Source.79: DFBPPR16006 ---- Animal proteins ---- Leptin Source.80: DFBPPR16223 ---- Animal proteins ---- Actin-histidine N-methyltransferase Source.81: DFBPPR16261 ---- Animal proteins ---- Retinoic acid receptor alpha Source.82: DFBPPR16270 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.83: DFBPPR16289 ---- Animal proteins ---- Amiloride-sensitive sodium channel subunit beta Source.84: DFBPPR16321 ---- Animal proteins ---- Aggrecan core protein Source.85: DFBPPR16482 ---- Animal proteins ---- Intercellular adhesion molecule 1 Source.86: DFBPPR16635 ---- Animal proteins ---- Corticoliberin Source.87: DFBPPR16641 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.88: DFBPPR16907 ---- Animal proteins ---- Acyl carrier protein, mitochondrial Source.89: DFBPPR16941 ---- Animal proteins ---- Sodium/potassium/calcium exchanger 1 Source.90: DFBPPR16987 ---- Animal proteins ---- Aggrecan core protein Source.91: DFBPPR17057 ---- Animal proteins ---- Cation-independent mannose-6-phosphate receptor Source.92: DFBPPR17268 ---- Animal proteins ---- Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Source.93: DFBPPR17316 ---- Animal proteins ---- Forkhead box protein O1 Source.94: DFBPPR17419 ---- Animal proteins ---- Inositol 1,4,5-triphosphate receptor associated 1 Source.95: DFBPPR17483 ---- Animal proteins ---- Speckle targeted PIP5K1A-regulated poly(A) polymerase Source.96: DFBPPR17485 ---- Animal proteins ---- B-cell antigen receptor complex-associated protein alpha chain Source.97: DFBPPR17490 ---- Animal proteins ---- TIR domain-containing adapter molecule 2 Source.98: DFBPPR17575 ---- Animal proteins ---- A disintegrin and metalloproteinase with thrombospondin motifs 4 Source.99: DFBPPR17849 ---- Animal proteins ---- Fibromodulin Source.100: DFBPPR17943 ---- Animal proteins ---- Transport and Golgi organization protein 1 homolog Source.101: DFBPPR18156 ---- Animal proteins ---- Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 Source.102: DFBPPR18866 ---- Animal proteins ---- Autophagy-related protein 9A Source.103: DFBPPR18889 ---- Animal proteins ---- Achaete-scute homolog 2 Source.104: DFBPPR18979 ---- Animal proteins ---- Alpha-2-macroglobulin Source.105: DFBPPR19033 ---- Animal proteins ---- Collagen alpha-2(XI) chain Source.106: DFBPPR19085 ---- Animal proteins ---- AP-4 complex subunit mu-1 Source.107: DFBPPR19190 ---- Animal proteins ---- Amiloride-sensitive sodium channel subunit gamma Source.108: DFBPPR19236 ---- Animal proteins ---- Alanine--glyoxylate aminotransferase 2, mitochondrial Source.109: DFBPPR19307 ---- Animal proteins ---- Microprocessor complex subunit DGCR8 Source.110: DFBPPR19412 ---- Animal proteins ---- N-terminal kinase-like protein Source.111: DFBPPR19489 ---- Animal proteins ---- Keratocan Source.112: DFBPPR19523 ---- Animal proteins ---- Origin recognition complex subunit 1 Source.113: DFBPPR20289 ---- Animal proteins ---- Di-N-acetylchitobiase Source.114: DFBPPR20418 ---- Animal proteins ---- Alpha-1B-glycoprotein Source.115: DFBPPR20440 ---- Animal proteins ---- 28S ribosomal protein S25, mitochondrial Source.116: DFBPPR20451 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.117: DFBPPR20609 ---- Animal proteins ---- Ran-binding protein 10 Source.118: DFBPPR20938 ---- Animal proteins ---- Serine protease HTR4 Source.119: DFBPPR21041 ---- Animal proteins ---- Cytokine-inducible SH2-containing protein Source.120: DFBPPR21057 ---- Animal proteins ---- KICSTOR complex protein ITFG2 Source.121: DFBPPR21073 ---- Animal proteins ---- 39S ribosomal protein L17, mitochondrial Source.122: DFBPPR21303 ---- Animal proteins ---- Palmdelphin Source.123: DFBPPR21361 ---- Animal proteins ---- Seizure protein 6 homolog Source.124: DFBPPR21408 ---- Animal proteins ---- Cytochrome c oxidase assembly protein COX15 homolog Source.125: DFBPPR21685 ---- Animal proteins ---- Prenylcysteine oxidase-like Source.126: DFBPPR21863 ---- Animal proteins ---- Ankyrin repeat and SOCS box protein 3 Source.127: DFBPPR21936 ---- Animal proteins ---- Peroxisomal membrane protein 2 Source.128: DFBPPR22069 ---- Animal proteins ---- Retrotransposon-like protein 1 Source.129: DFBPPR22098 ---- Animal proteins ---- Esterase OVCA2 Source.130: DFBPPR22273 ---- Animal proteins ---- 39S ribosomal protein L45, mitochondrial Source.131: DFBPPR22492 ---- Animal proteins ---- SAYSvFN domain-containing protein 1 Source.132: DFBPPR22493 ---- Animal proteins ---- PC-esterase domain-containing protein 1B Source.133: DFBPPR22496 ---- Animal proteins ---- Dysbindin domain-containing protein 1 Source.134: DFBPPR22598 ---- Animal proteins ---- UPF0488 protein C8orf33 homolog Source.135: DFBPPR22607 ---- Animal proteins ---- Transmembrane protein 128 Source.136: DFBPPR22651 ---- Animal proteins ---- Spermatogenesis-associated protein 2-like protein Source.137: DFBPPR8543 ---- Animal proteins ---- Sterol regulatory element-binding protein 1 Source.138: DFBPPR8712 ---- Animal proteins ---- Interferon-induced GTP-binding protein Mx2 Source.139: DFBPPR8741 ---- Animal proteins ---- Forkhead box protein O1 Source.140: DFBPPR9085 ---- Animal proteins ---- Membrane-associated progesterone receptor component 1 Source.141: DFBPPR9169 ---- Animal proteins ---- Aggrecan core protein Source.142: DFBPPR9759 ---- Animal proteins ---- Palmdelphin Source.143: DFBPPR9764 ---- Animal proteins ---- Cationic amino acid transporter 2 Source.144: DFBPPR9851 ---- Animal proteins ---- Fibromodulin Source.145: DFBPPR9927 ---- Animal proteins ---- Protein BTG3 Source.146: DFBPPR9986 ---- Animal proteins ---- Aggrecan core protein Source.147: DFBPPR10155 ---- Animal proteins ---- Cationic amino acid transporter 2 Source.148: DFBPPR10173 ---- Animal proteins ---- Teneurin-1 Source.149: DFBPPR10178 ---- Animal proteins ---- Homeobox protein SIX3 Source.150: DFBPPR10223 ---- Animal proteins ---- Retinoic acid receptor alpha Source.151: DFBPPR10230 ---- Animal proteins ---- B-cell linker protein Source.152: DFBPPR10318 ---- Animal proteins ---- LIM domain kinase 1 Source.153: DFBPPR10548 ---- Animal proteins ---- Syndecan-4 Source.154: DFBPPR10753 ---- Animal proteins ---- Hypermethylated in cancer 1 protein Source.155: DFBPPR10940 ---- Animal proteins ---- Protein piccolo Source.156: DFBPPR11172 ---- Animal proteins ---- Protein transport protein Sec16B Source.157: DFBPPR11406 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 47 Source.158: DFBPPR11434 ---- Animal proteins ---- Homeobox protein SIX6 Source.159: DFBPPR11461 ---- Animal proteins ---- Solute carrier family 25 member 46 Source.160: DFBPPR11467 ---- Animal proteins ---- Synembryn-A Source.161: DFBPPR11471 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 11 Source.162: DFBPPR11526 ---- Animal proteins ---- Fibromodulin Source.163: DFBPPR11571 ---- Animal proteins ---- Alpha-(1,3)-fucosyltransferase 10 Source.164: DFBPPR11653 ---- Animal proteins ---- Erythroblast NAD(P)(+)--arginine ADP-ribosyltransferase Source.165: DFBPPR11705 ---- Animal proteins ---- SCO-spondin Source.166: DFBPPR11769 ---- Animal proteins ---- Cytokine-inducible SH2-containing protein Source.167: DFBPPR11958 ---- Animal proteins ---- Homeobox protein engrailed-1 Source.168: DFBPPR12044 ---- Animal proteins ---- 39S ribosomal protein L37, mitochondrial Source.169: DFBPPR12234 ---- Animal proteins ---- Rab9 effector protein with kelch motifs Source.170: DFBPPR12269 ---- Animal proteins ---- Potassium voltage-gated channel subfamily A member 5 Source.171: DFBPPR12401 ---- Animal proteins ---- Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Source.172: DFBPPR12451 ---- Animal proteins ---- Non-specific lipid-transfer protein Source.173: DFBPPR12592 ---- Animal proteins ---- Amiloride-sensitive sodium channel subunit beta Source.174: DFBPPR12896 ---- Animal proteins ---- T-lymphocyte activation antigen CD80 Source.175: DFBPPR13041 ---- Animal proteins ---- Fibromodulin Source.176: DFBPPR13077 ---- Animal proteins ---- Collagen alpha-1(XII) chain Source.177: DFBPPR13091 ---- Animal proteins ---- Cortactin-binding protein 2 Source.178: DFBPPR13155 ---- Animal proteins ---- C-C motif chemokine 5 Source.179: DFBPPR13239 ---- Animal proteins ---- T-cell surface antigen CD2 Source.180: DFBPPR13406 ---- Animal proteins ---- Cortactin-binding protein 2 Source.181: DFBPPR13870 ---- Animal proteins ---- Cathelicidin-3 Source.182: DFBPPR14019 ---- Animal proteins ---- Vimentin Source.183: DFBPPR14975 ---- Microorganism protein ---- Inner kinetochore subunit CTF19 Source.184: DFBPPR15006 ---- Microorganism protein ---- Lysophospholipase NTE1 Source.185: DFBPPR15212 ---- Microorganism protein ---- Protein transport protein SEC23 Source.186: DFBPPR15733 ---- Microorganism protein ---- J protein JJJ2 Source.187: DFBPPR7826 ---- Plant protein ---- U1 small nuclear ribonucleoprotein C-2 Source.188: DFBPPR7827 ---- Plant protein ---- U1 small nuclear ribonucleoprotein C-1 Source.189: DFBPPR7867 ---- Plant protein ---- CASP-like protein 3A1 Source.190: DFBPPR8040 ---- Plant protein ---- Nitrate reductase [NADH] 1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Ileum contracting activity

The peptide PLPR exhibits weak stimulatory activity on ileal contraction.

Sequence		Rapid		Slow
Sequence		EC₅₀ ± SE (μM)	n	EC₅₀ ± SE (μM)	n
C3a (70-77)	ASHLGLAR	16 ± 5.1	4	1.6 ± 0.40	4
Oryzatensin	GYPMYPLPR	4.5 ± 1.6	3	0.082 ± 0.019	4
	YPMYPLPR	3.7 ± 1.2	3	0.055 ± 0.017	3
	PMYPLPR	18 ± 4.5	3	0.76 ± 0.11	4
	MYPLPR	33 ± 13	3	1.2 ± 0.19	3
	YPLPR	50 ± 3.3	3	2.2 ± 0.82	3
	PLPR	*	2	59 ± 15	3
	YPMYPLP	*	2	*	2
The numbers of guinea pigs used are indicated by n. * No contraction was observed at 1 mM.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Peptides used in this study were synthesized by the Fmoc strategy using a Sam 2 peptide synthesizer (Biosearch Inc.). The peptides were purified by reversed-phase HPLC on an octadecyl silica column.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Takahashi M, Moriguchi S, Ikeno M, Kono S, Ohata K, Usui H, Kurahashi K, Sasaki R, Yoshikawa M. Studies on the ileum-contracting mechanisms and identification as a complement C3a receptor agonist of oryzatensin, a bioactive peptide derived from rice albumin. Peptides. 1996;17(1):5-12. PMID: 8822503 DOI: 10.1016/0196-9781(95)02059-4
Other literature(s)	N.D
PubDate	1996