Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPIMMU0001(Immunomodulatory peptide)

DFBP ID	DFBPIMMU0001
Peptide sequence	VEPIPY
Type	Native peptide
Peptide/Function name	Immunomodulatory peptide

Function-activity relationship

Main bioactivity	Immunomodulatory activity
Otheir bioactivity	PEP-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Val-Glu-Pro-Ile-Pro-Tyr

Single-letter amino acid

VEPIPY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	716.82 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.1167^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk
Precursor protein	β-Casein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR7605 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	Link 1: DFBPIMMU0053----Casein----β-Casein

Biological/Functional activity & target protein

Immunomodulatory activity	Stimulated the phagocytosis of opsonized sheep red blood cells (SRBCs) by murine peritoneal macrophages and enhanced the resistance of mice to infection withKlehsiella pneumoniae.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Structure

Embedded Mol* Viewer

Docking

Embedded Mol* Viewer

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPPEPI0071
[D2]	DFBPMUFU0755

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Parker F, Migliore-Samour D, Floc'h F, Zerial A, Werner GH, Jollès J, Casaretto M, Zahn H, Jollès P. Immunostimulating hexapeptide from human casein: amino acid sequence, synthesis and biological properties. Eur J Biochem. 1984 Dec 17;145(3):677-82. PMID: 6548963 DOI: 10.1111/j.1432-1033.1984.tb08609.x
Other literature(s)	[1] Chalamaiah M, Yu W, Wu J. Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review.[J]. Food Chemistry, 2018, 245:205-222. [2] Bhattacharya M, Puri A, Sahai R, et al. Immunosuppressant activity in human beta-casein fragment analogs.[J]. Bioorganic & Medicinal Chemistry Letters, 1998, 8(4):327-332.
PubDate	1984