Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPIMMU0022(Immunomodulatory peptide)

DFBP ID	DFBPIMMU0022
Peptide sequence	EGAK
Type	Native peptide
Peptide/Function name	Immunomodulatory peptide

Function-activity relationship

Main bioactivity	Immunomodulatory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Gly-Ala-Lys

Single-letter amino acid

EGAK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	403.43 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.5000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Paphia undulata
Precursor protein	Paphia undulata meat protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0845 ---- Plant proteins ---- Guanine nucleotide-binding protein alpha-1 subunit Source.2: DFBPPR0879 ---- Plant proteins ---- UDP-arabinopyranose mutase 1 Source.3: DFBPPR0933 ---- Plant proteins ---- ATP-dependent RNA helicase SUV3, mitochondrial Source.4: DFBPPR1245 ---- Plant proteins ---- TPR repeat-containing protein ZIP4 Source.5: DFBPPR1326 ---- Plant proteins ---- Protein SHORTAGE IN CHIASMATA 1 homolog Source.6: DFBPPR1572 ---- Plant proteins ---- Late embryogenesis abundant protein 17 Source.7: DFBPPR1646 ---- Plant proteins ---- ADP,ATP carrier protein, mitochondrial Source.8: DFBPPR1672 ---- Plant proteins ---- Alcohol dehydrogenase class-3 Source.9: DFBPPR1705 ---- Plant proteins ---- Probable GTP-binding protein OBGC1, chloroplastic Source.10: DFBPPR1723 ---- Plant proteins ---- ATP synthase subunit 9, mitochondrial Source.11: DFBPPR1776 ---- Plant proteins ---- Lon protease homolog, mitochondrial Source.12: DFBPPR1860 ---- Plant proteins ---- Kinesin-like protein KIN-7A Source.13: DFBPPR2248 ---- Plant proteins ---- Membrane steroid-binding protein 1 Source.14: DFBPPR3073 ---- Plant proteins ---- Kinesin-like protein KIN-7H Source.15: DFBPPR3299 ---- Plant proteins ---- Metal transporter Nramp4 Source.16: DFBPPR3334 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL12 Source.17: DFBPPR3683 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL14 Source.18: DFBPPR4026 ---- Plant proteins ---- Potassium transporter 19 Source.19: DFBPPR4027 ---- Plant proteins ---- Potassium transporter 20 Source.20: DFBPPR4149 ---- Plant proteins ---- Probable anion transporter 7 Source.21: DFBPPR4244 ---- Plant proteins ---- Flotillin-like protein 1 Source.22: DFBPPR4603 ---- Plant proteins ---- Tubby-like F-box protein 2 Source.23: DFBPPR4745 ---- Plant proteins ---- BURP domain-containing protein 9 Source.24: DFBPPR4910 ---- Plant proteins ---- Calcium-dependent protein kinase 10 Source.25: DFBPPR5084 ---- Plant proteins ---- ATP synthase subunit 9, mitochondrial Source.26: DFBPPR5183 ---- Plant proteins ---- Histone deacetylase HDT1 Source.27: DFBPPR5196 ---- Plant proteins ---- Arginase Source.28: DFBPPR5234 ---- Plant proteins ---- 17.9 kDa class II heat shock protein Source.29: DFBPPR5337 ---- Plant proteins ---- Heat shock 22 kDa protein, mitochondrial Source.30: DFBPPR5538 ---- Plant proteins ---- Alcohol dehydrogenase class-3 Source.31: DFBPPR5575 ---- Plant proteins ---- ATP synthase subunit 9, mitochondrial Source.32: DFBPPR5584 ---- Plant proteins ---- GRF-interacting factor 10 Source.33: DFBPPR6235 ---- Plant proteins ---- Alcohol dehydrogenase class-3 Source.34: DFBPPR6346 ---- Plant proteins ---- ATP synthase subunit 9, mitochondrial Source.35: DFBPPR6352 ---- Plant proteins ---- Protein TIC 22, chloroplastic Source.36: DFBPPR6573 ---- Plant proteins ---- Heat shock 22 kDa protein, mitochondrial Source.37: DFBPPR6679 ---- Plant proteins ---- ATP synthase subunit 9, mitochondrial Source.38: DFBPPR6757 ---- Plant proteins ---- ADP,ATP carrier protein 2, mitochondrial Source.39: DFBPPR6758 ---- Plant proteins ---- ADP,ATP carrier protein 1, mitochondrial Source.40: DFBPPR7423 ---- Plant proteins ---- ATP synthase subunit 9, mitochondrial Source.41: DFBPPR15972 ---- Animal proteins ---- Catenin beta-1 Source.42: DFBPPR15979 ---- Animal proteins ---- Serine/threonine-protein kinase NLK Source.43: DFBPPR16108 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Source.44: DFBPPR16602 ---- Animal proteins ---- Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial Source.45: DFBPPR16624 ---- Animal proteins ---- Vascular cell adhesion protein 1 Source.46: DFBPPR16857 ---- Animal proteins ---- Plasma serine protease inhibitor Source.47: DFBPPR16958 ---- Animal proteins ---- Retinal dehydrogenase 1 Source.48: DFBPPR17053 ---- Animal proteins ---- Junction plakoglobin Source.49: DFBPPR17091 ---- Animal proteins ---- Extracellular calcium-sensing receptor Source.50: DFBPPR17267 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 4B Source.51: DFBPPR17285 ---- Animal proteins ---- Serine/threonine-protein kinase N1 Source.52: DFBPPR17464 ---- Animal proteins ---- Aldehyde dehydrogenase, mitochondrial Source.53: DFBPPR18083 ---- Animal proteins ---- Developmentally-regulated GTP-binding protein 1 Source.54: DFBPPR18152 ---- Animal proteins ---- Mitochondrial 2-oxoglutarate/malate carrier protein Source.55: DFBPPR18153 ---- Animal proteins ---- Mitochondrial 2-oxoglutarate/malate carrier protein Source.56: DFBPPR18249 ---- Animal proteins ---- Argininosuccinate synthase Source.57: DFBPPR18305 ---- Animal proteins ---- Aldehyde dehydrogenase X, mitochondrial Source.58: DFBPPR18320 ---- Animal proteins ---- Serine/threonine-protein kinase NLK Source.59: DFBPPR18450 ---- Animal proteins ---- ADP/ATP translocase 2 Source.60: DFBPPR18479 ---- Animal proteins ---- Pyruvate dehydrogenase protein X component Source.61: DFBPPR18549 ---- Animal proteins ---- Serum amyloid A protein Source.62: DFBPPR18572 ---- Animal proteins ---- Protein-L-isoaspartate(D-aspartate) O-methyltransferase Source.63: DFBPPR18865 ---- Animal proteins ---- Collagen alpha-1(XI) chain Source.64: DFBPPR19424 ---- Animal proteins ---- 3-hydroxybutyrate dehydrogenase type 2 Source.65: DFBPPR19518 ---- Animal proteins ---- Rab GTPase-binding effector protein 2 Source.66: DFBPPR20028 ---- Animal proteins ---- Sine oculis-binding protein homolog Source.67: DFBPPR20043 ---- Animal proteins ---- Pre-B-cell leukemia transcription factor-interacting protein 1 Source.68: DFBPPR20129 ---- Animal proteins ---- 2-aminomuconic semialdehyde dehydrogenase Source.69: DFBPPR20181 ---- Animal proteins ---- Choline transporter-like protein 2 Source.70: DFBPPR20193 ---- Animal proteins ---- T-complex protein 1 subunit theta Source.71: DFBPPR20265 ---- Animal proteins ---- Histone-lysine N-methyltransferase EZH1 Source.72: DFBPPR20535 ---- Animal proteins ---- FAD-dependent oxidoreductase domain-containing protein 1 Source.73: DFBPPR20718 ---- Animal proteins ---- Homeobox protein MSX-1 Source.74: DFBPPR20737 ---- Animal proteins ---- Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial Source.75: DFBPPR21011 ---- Animal proteins ---- Solute carrier family 35 member B1 Source.76: DFBPPR21040 ---- Animal proteins ---- Neuritin Source.77: DFBPPR21290 ---- Animal proteins ---- Metaxin-1 Source.78: DFBPPR21474 ---- Animal proteins ---- m-AAA protease-interacting protein 1, mitochondrial Source.79: DFBPPR21478 ---- Animal proteins ---- FTS and Hook-interacting protein Source.80: DFBPPR21766 ---- Animal proteins ---- Dehydrogenase/reductase SDR family member 12 Source.81: DFBPPR21970 ---- Animal proteins ---- Testis-specific Y-encoded-like protein 1 Source.82: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.83: DFBPPR8908 ---- Animal proteins ---- Extracellular calcium-sensing receptor Source.84: DFBPPR9140 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Source.85: DFBPPR9250 ---- Animal proteins ---- Junction plakoglobin Source.86: DFBPPR9440 ---- Animal proteins ---- Aldehyde dehydrogenase, mitochondrial Source.87: DFBPPR9441 ---- Animal proteins ---- Aldehyde dehydrogenase, mitochondrial Source.88: DFBPPR9566 ---- Animal proteins ---- Choline transporter-like protein 2 Source.89: DFBPPR9594 ---- Animal proteins ---- Protein-L-isoaspartate(D-aspartate) O-methyltransferase Source.90: DFBPPR9910 ---- Animal proteins ---- Serum amyloid A-2 protein Source.91: DFBPPR10008 ---- Animal proteins ---- Protein Wnt-2b Source.92: DFBPPR10261 ---- Animal proteins ---- Cadherin-13 Source.93: DFBPPR10347 ---- Animal proteins ---- Proto-oncogene tyrosine-protein kinase LCK Source.94: DFBPPR10443 ---- Animal proteins ---- Retinal dehydrogenase 2 Source.95: DFBPPR10470 ---- Animal proteins ---- Collagen alpha-1(III) chain Source.96: DFBPPR10644 ---- Animal proteins ---- UDP-glucose 6-dehydrogenase Source.97: DFBPPR10717 ---- Animal proteins ---- Tyrosine-protein phosphatase non-receptor type 1 Source.98: DFBPPR10847 ---- Animal proteins ---- Protein patched homolog 1 Source.99: DFBPPR10992 ---- Animal proteins ---- Retinal dehydrogenase 1 Source.100: DFBPPR11186 ---- Animal proteins ---- T-complex protein 1 subunit theta Source.101: DFBPPR11317 ---- Animal proteins ---- Dickkopf-related protein 3 Source.102: DFBPPR11422 ---- Animal proteins ---- Methionine aminopeptidase 1 Source.103: DFBPPR11662 ---- Animal proteins ---- Protein-L-isoaspartate(D-aspartate) O-methyltransferase Source.104: DFBPPR11671 ---- Animal proteins ---- Glucoside xylosyltransferase 1 Source.105: DFBPPR11709 ---- Animal proteins ---- Xin actin-binding repeat-containing protein 1 Source.106: DFBPPR11929 ---- Animal proteins ---- Probable RNA-binding protein EIF1AD Source.107: DFBPPR11972 ---- Animal proteins ---- Cyclin-L1 Source.108: DFBPPR12275 ---- Animal proteins ---- Ryanodine receptor 3 Source.109: DFBPPR12281 ---- Animal proteins ---- Ryanodine receptor 1 Source.110: DFBPPR12290 ---- Animal proteins ---- Ryanodine receptor 2 Source.111: DFBPPR12297 ---- Animal proteins ---- Retinal dehydrogenase 1 Source.112: DFBPPR12364 ---- Animal proteins ---- Protein Wnt-5a Source.113: DFBPPR12626 ---- Animal proteins ---- E-selectin Source.114: DFBPPR12650 ---- Animal proteins ---- ADP/ATP translocase 1 Source.115: DFBPPR12900 ---- Animal proteins ---- Solute carrier family 22 member 6 Source.116: DFBPPR13190 ---- Animal proteins ---- Sodium channel protein type 4 subunit alpha Source.117: DFBPPR13206 ---- Animal proteins ---- Retinal dehydrogenase 1 Source.118: DFBPPR13254 ---- Animal proteins ---- Phosphoglycerate kinase 1 Source.119: DFBPPR13283 ---- Animal proteins ---- Aldehyde dehydrogenase, mitochondrial Source.120: DFBPPR13293 ---- Animal proteins ---- Phosphoglycerate kinase 2 Source.121: DFBPPR13533 ---- Animal proteins ---- Retinal dehydrogenase 1 Source.122: DFBPPR13805 ---- Animal proteins ---- Serum amyloid A protein Source.123: DFBPPR15077 ---- Microorganism protein ---- Endopolyphosphatase Source.124: DFBPPR15317 ---- Microorganism protein ---- Phosphatidylinositol transfer protein SFH5 Source.125: DFBPPR15690 ---- Microorganism protein ---- Inclusion body clearance protein IML2 Source.126: DFBPPR15740 ---- Microorganism protein ---- Altered inheritance of mitochondria protein 21 Source.127: DFBPPR15806 ---- Microorganism protein ---- Malonate-semialdehyde dehydrogenase Source.128: DFBPPR15815 ---- Microorganism protein ---- Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase Source.129: DFBPPR7941 ---- Plant protein ---- ATP synthase subunit 9, mitochondrial Source.130: DFBPPR8232 ---- Plant protein ---- ATP synthase subunit 9, mitochondrial Source.131: DFBPPR8234 ---- Plant protein ---- Anther-specific protein SF18
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Immunomodulatory activity	Significantly enhanced mice lymphocyte proliferation ability in ex vivo.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with alcalase.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	He XQ, Cao WH, Pan GK, Yang L, Zhang CH. Enzymatic hydrolysis optimization of Paphia undulata and lymphocyte proliferation activity of the isolated peptide fractions. J Sci Food Agric. 2015 May;95(7):1544-53. PMID: 25087732 DOI: 10.1002/jsfa.6859
Other literature(s)	[1] Chalamaiah M, Yu W, Wu J. Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review.[J]. Food Chemistry, 2018, 245:205-222.
PubDate	2015