Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPIMMU0059(Immunomodulatory peptide)

DFBP ID	DFBPIMMU0059
Peptide sequence	GRKP
Type	Native peptide
Peptide/Function name	Immunomodulatory peptide

Function-activity relationship

Main bioactivity	Immunomodulatory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Arg-Lys-Pro

Single-letter amino acid

GRKP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
457.0 Da	456.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.54 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.6000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Growth factor b(TGFb)family
Precursor protein	TGFb protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1148 ---- Plant proteins ---- L-type lectin-domain containing receptor kinase SIT2 Source.2: DFBPPR1221 ---- Plant proteins ---- Probable GTP diphosphokinase CRSH1, chloroplastic Source.3: DFBPPR1248 ---- Plant proteins ---- Glycosyltransferase BC10 Source.4: DFBPPR1417 ---- Plant proteins ---- DnaJ protein ERDJ3A Source.5: DFBPPR1426 ---- Plant proteins ---- Mitogen-activated protein kinase 4 Source.6: DFBPPR1478 ---- Plant proteins ---- Linoleate 9S-lipoxygenase 2 Source.7: DFBPPR1625 ---- Plant proteins ---- Mitogen-activated protein kinase 3 Source.8: DFBPPR1765 ---- Plant proteins ---- Transcription factor MYC2 Source.9: DFBPPR1904 ---- Plant proteins ---- Alanine--tRNA ligase, chloroplastic/mitochondrial Source.10: DFBPPR2002 ---- Plant proteins ---- bZIP transcription factor 60 Source.11: DFBPPR2646 ---- Plant proteins ---- CBL-interacting protein kinase 25 Source.12: DFBPPR2807 ---- Plant proteins ---- Beta-glucosidase 32 Source.13: DFBPPR3024 ---- Plant proteins ---- Beta-glucosidase 31 Source.14: DFBPPR3070 ---- Plant proteins ---- Putative D-cysteine desulfhydrase 1, mitochondrial Source.15: DFBPPR3560 ---- Plant proteins ---- Probable inactive beta-glucosidase 33 Source.16: DFBPPR3671 ---- Plant proteins ---- Putative linoleate 9S-lipoxygenase 3 Source.17: DFBPPR3968 ---- Plant proteins ---- CRS2-associated factor 1, chloroplastic Source.18: DFBPPR4080 ---- Plant proteins ---- Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic Source.19: DFBPPR4177 ---- Plant proteins ---- Formin-like protein 16 Source.20: DFBPPR4211 ---- Plant proteins ---- Probable GTP-binding protein OBGM, mitochondrial Source.21: DFBPPR4891 ---- Plant proteins ---- Isoamylase 1, chloroplastic Source.22: DFBPPR4977 ---- Plant proteins ---- Leucine-rich repeat receptor-like kinase protein CLV1B Source.23: DFBPPR4979 ---- Plant proteins ---- Seed linoleate 9S-lipoxygenase-3 Source.24: DFBPPR5013 ---- Plant proteins ---- Leucine-rich repeat receptor-like kinase protein CLV1a Source.25: DFBPPR5138 ---- Plant proteins ---- Subtilisin-like protease Glyma18g48580 Source.26: DFBPPR5762 ---- Plant proteins ---- CRS2-associated factor 1, chloroplastic Source.27: DFBPPR6247 ---- Plant proteins ---- DNA topoisomerase 2 Source.28: DFBPPR6370 ---- Plant proteins ---- Seed linoleate 9S-lipoxygenase-3 Source.29: DFBPPR7013 ---- Plant proteins ---- Protein MLO Source.30: DFBPPR7616 ---- Milk proteins ---- Macrophage mannose receptor 1 Source.31: DFBPPR15987 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.32: DFBPPR16119 ---- Animal proteins ---- High mobility group protein HMG-I/HMG-Y Source.33: DFBPPR16139 ---- Animal proteins ---- Lipocalin Can f 6.0101 Source.34: DFBPPR16237 ---- Animal proteins ---- Mitochondrial brown fat uncoupling protein 1 Source.35: DFBPPR16465 ---- Animal proteins ---- Myotrophin Source.36: DFBPPR16950 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.37: DFBPPR17074 ---- Animal proteins ---- Group 3 secretory phospholipase A2 Source.38: DFBPPR17739 ---- Animal proteins ---- Molybdenum cofactor biosynthesis protein 1 Source.39: DFBPPR17995 ---- Animal proteins ---- Ferrochelatase, mitochondrial Source.40: DFBPPR18003 ---- Animal proteins ---- 7-dehydrocholesterol reductase Source.41: DFBPPR18207 ---- Animal proteins ---- Interleukin-7 Source.42: DFBPPR18317 ---- Animal proteins ---- G-protein coupled receptor 37-like 1 Source.43: DFBPPR18323 ---- Animal proteins ---- Transcription factor E2F7 Source.44: DFBPPR18430 ---- Animal proteins ---- cAMP-responsive element-binding protein-like 2 Source.45: DFBPPR18502 ---- Animal proteins ---- Insulin-like growth factor-binding protein 2 Source.46: DFBPPR18800 ---- Animal proteins ---- Transcription factor E2F8 Source.47: DFBPPR18918 ---- Animal proteins ---- Sodium-coupled neutral amino acid transporter 5 Source.48: DFBPPR19055 ---- Animal proteins ---- Complement factor D Source.49: DFBPPR19228 ---- Animal proteins ---- Myotrophin Source.50: DFBPPR19523 ---- Animal proteins ---- Origin recognition complex subunit 1 Source.51: DFBPPR19642 ---- Animal proteins ---- Agouti-related protein Source.52: DFBPPR19659 ---- Animal proteins ---- 39S ribosomal protein L9, mitochondrial Source.53: DFBPPR19893 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase isozyme L3 Source.54: DFBPPR20272 ---- Animal proteins ---- Fatty acyl-CoA reductase 2 Source.55: DFBPPR21013 ---- Animal proteins ---- TATA box-binding protein-associated factor RNA polymerase I subunit D Source.56: DFBPPR21224 ---- Animal proteins ---- Smoothelin-like protein 2 Source.57: DFBPPR21248 ---- Animal proteins ---- 39S ribosomal protein L4, mitochondrial Source.58: DFBPPR21373 ---- Animal proteins ---- Zinc finger protein 2 Source.59: DFBPPR22127 ---- Animal proteins ---- Tumor protein p53-inducible protein 11 Source.60: DFBPPR22143 ---- Animal proteins ---- Hippocampus abundant transcript-like protein 1 Source.61: DFBPPR22302 ---- Animal proteins ---- Protein angel homolog 2 Source.62: DFBPPR22347 ---- Animal proteins ---- Etoposide-induced protein 2.4 homolog Source.63: DFBPPR8531 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.64: DFBPPR8974 ---- Animal proteins ---- ADM5 Source.65: DFBPPR9079 ---- Animal proteins ---- Prolactin receptor Source.66: DFBPPR9280 ---- Animal proteins ---- Insulin-like growth factor-binding protein 2 Source.67: DFBPPR9553 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase isozyme L3 Source.68: DFBPPR9707 ---- Animal proteins ---- Interleukin-7 Source.69: DFBPPR9739 ---- Animal proteins ---- Solute carrier family 22 member 8 Source.70: DFBPPR10692 ---- Animal proteins ---- Protein Wnt-9a Source.71: DFBPPR10876 ---- Animal proteins ---- Telomere-associated protein RIF1 Source.72: DFBPPR10940 ---- Animal proteins ---- Protein piccolo Source.73: DFBPPR11007 ---- Animal proteins ---- Anamorsin Source.74: DFBPPR11268 ---- Animal proteins ---- Myotrophin Source.75: DFBPPR12374 ---- Animal proteins ---- Sortilin-related receptor Source.76: DFBPPR12750 ---- Animal proteins ---- Anion exchange protein 2 Source.77: DFBPPR12943 ---- Animal proteins ---- Solute carrier family 22 member 8 Source.78: DFBPPR13150 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.79: DFBPPR13239 ---- Animal proteins ---- T-cell surface antigen CD2 Source.80: DFBPPR13272 ---- Animal proteins ---- Carnitine O-palmitoyltransferase 1, liver isoform Source.81: DFBPPR13541 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.82: DFBPPR13671 ---- Animal proteins ---- Interleukin-7 Source.83: DFBPPR13746 ---- Animal proteins ---- Insulin-like growth factor-binding protein 2 Source.84: DFBPPR13894 ---- Animal proteins ---- Brain-enriched guanylate kinase-associated protein Source.85: DFBPPR14401 ---- Marine protein ---- 50S ribosomal protein L4, chloroplastic Source.86: DFBPPR15014 ---- Microorganism protein ---- AP-1-like transcription factor YAP1 Source.87: DFBPPR15159 ---- Microorganism protein ---- Centromere-binding protein 1 Source.88: DFBPPR15274 ---- Microorganism protein ---- ATP-dependent RNA helicase DBP7 Source.89: DFBPPR15294 ---- Microorganism protein ---- Lactose permease Source.90: DFBPPR7785 ---- Plant protein ---- Alanine--tRNA ligase, chloroplastic/mitochondrial
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Immunomodulatory activity	The peptide showed immunomodulatory activity.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	All the peptides were synthesized by solid-phase methods, using chloromethylated copolymer of styrene and 1% divinyl benzene.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wieczorek Z, Słoń J, Kluczyk A, Zbozień R, Stafanowicz P, Siemion IZ. The immunomodulatory diversity of the proteins of the transforming growth factor beta (TGF beta) family. Int J Pept Protein Res. 1995 Aug;46(2):113-8. PMID: 8567165 DOI: 10.1111/j.1399-3011.1995.tb01326.x
Other literature(s)	N.D
PubDate	1995