Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPIMMU0060(Immunomodulatory peptide)

DFBP ID	DFBPIMMU0060
Peptide sequence	RKPK
Type	Native peptide
Peptide/Function name	Immunomodulatory peptide

Function-activity relationship

Main bioactivity	Immunomodulatory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Arg-Lys-Pro-Lys

Single-letter amino acid

RKPK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
529.2 Da	527.66 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.68 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-3.4750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Growth factor b(TGFb)family
Precursor protein	TGFb protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0929 ---- Plant proteins ---- Protein ROS1A Source.2: DFBPPR1109 ---- Plant proteins ---- Origin of replication complex subunit 1 Source.3: DFBPPR2343 ---- Plant proteins ---- Protein kinase G11A Source.4: DFBPPR2692 ---- Plant proteins ---- FACT complex subunit SSRP1-A Source.5: DFBPPR2892 ---- Plant proteins ---- 30S ribosomal protein S18, chloroplastic Source.6: DFBPPR2999 ---- Plant proteins ---- FACT complex subunit SSRP1-B Source.7: DFBPPR4211 ---- Plant proteins ---- Probable GTP-binding protein OBGM, mitochondrial Source.8: DFBPPR4301 ---- Plant proteins ---- Protein argonaute 16 Source.9: DFBPPR6706 ---- Plant proteins ---- Adenine phosphoribosyltransferase 1 Source.10: DFBPPR15987 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.11: DFBPPR16031 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit Source.12: DFBPPR16064 ---- Animal proteins ---- Calnexin Source.13: DFBPPR16082 ---- Animal proteins ---- Ras-related protein Rab-9A Source.14: DFBPPR16187 ---- Animal proteins ---- Dual serine/threonine and tyrosine protein kinase Source.15: DFBPPR16256 ---- Animal proteins ---- Transcription factor GATA-4 Source.16: DFBPPR16950 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.17: DFBPPR17304 ---- Animal proteins ---- Endoplasmic reticulum membrane sensor NFE2L1 Source.18: DFBPPR17591 ---- Animal proteins ---- 72 kDa type IV collagenase Source.19: DFBPPR17801 ---- Animal proteins ---- Gamma-aminobutyric acid receptor subunit rho-2 Source.20: DFBPPR17930 ---- Animal proteins ---- Dual serine/threonine and tyrosine protein kinase Source.21: DFBPPR18003 ---- Animal proteins ---- 7-dehydrocholesterol reductase Source.22: DFBPPR18965 ---- Animal proteins ---- THO complex subunit 5 homolog Source.23: DFBPPR19227 ---- Animal proteins ---- Nuclear speckle splicing regulatory protein 1 Source.24: DFBPPR19317 ---- Animal proteins ---- AP-1 complex subunit sigma-1A Source.25: DFBPPR19513 ---- Animal proteins ---- Homeobox protein EMX2 Source.26: DFBPPR19834 ---- Animal proteins ---- Harmonin Source.27: DFBPPR19838 ---- Animal proteins ---- Transcription factor GATA-5 Source.28: DFBPPR19942 ---- Animal proteins ---- AP-1 complex subunit sigma-2 Source.29: DFBPPR20325 ---- Animal proteins ---- 28S ribosomal protein S12, mitochondrial Source.30: DFBPPR20524 ---- Animal proteins ---- U1 small nuclear ribonucleoprotein A Source.31: DFBPPR21633 ---- Animal proteins ---- DNA fragmentation factor subunit beta Source.32: DFBPPR21837 ---- Animal proteins ---- Zinc finger protein 750 Source.33: DFBPPR21861 ---- Animal proteins ---- Succinate dehydrogenase assembly factor 3, mitochondrial Source.34: DFBPPR22127 ---- Animal proteins ---- Tumor protein p53-inducible protein 11 Source.35: DFBPPR22247 ---- Animal proteins ---- NXPE family member 3 Source.36: DFBPPR22599 ---- Animal proteins ---- Tetratricopeptide repeat protein 9C Source.37: DFBPPR22610 ---- Animal proteins ---- Transmembrane protein 215 Source.38: DFBPPR8531 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.39: DFBPPR8742 ---- Animal proteins ---- Low-density lipoprotein receptor-related protein 2 Source.40: DFBPPR8945 ---- Animal proteins ---- Coagulation factor V Source.41: DFBPPR9534 ---- Animal proteins ---- Transcription factor GATA-6 Source.42: DFBPPR9589 ---- Animal proteins ---- U1 small nuclear ribonucleoprotein A Source.43: DFBPPR10006 ---- Animal proteins ---- Toll-like receptor 2 type-1 Source.44: DFBPPR10035 ---- Animal proteins ---- 72 kDa type IV collagenase Source.45: DFBPPR10266 ---- Animal proteins ---- Transcription factor GATA-6 Source.46: DFBPPR10293 ---- Animal proteins ---- Toll-like receptor 2 type-2 Source.47: DFBPPR10311 ---- Animal proteins ---- Homeobox protein engrailed-2 Source.48: DFBPPR10411 ---- Animal proteins ---- DNA-dependent protein kinase catalytic subunit Source.49: DFBPPR10471 ---- Animal proteins ---- Transcription factor SOX-21 Source.50: DFBPPR10693 ---- Animal proteins ---- Ferrochelatase, mitochondrial Source.51: DFBPPR10739 ---- Animal proteins ---- Transcription factor SOX-14 Source.52: DFBPPR10894 ---- Animal proteins ---- Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 Source.53: DFBPPR11021 ---- Animal proteins ---- Protein Jumonji Source.54: DFBPPR11136 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase gamma Source.55: DFBPPR11446 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 48 Source.56: DFBPPR11477 ---- Animal proteins ---- Transcription factor GATA-5 Source.57: DFBPPR11502 ---- Animal proteins ---- Transcription factor GATA-4 Source.58: DFBPPR11629 ---- Animal proteins ---- THO complex subunit 5 homolog Source.59: DFBPPR11697 ---- Animal proteins ---- N-alpha-acetyltransferase 35, NatC auxiliary subunit Source.60: DFBPPR11831 ---- Animal proteins ---- Protein lin-28 homolog B Source.61: DFBPPR11951 ---- Animal proteins ---- Integrator complex subunit 2 Source.62: DFBPPR11954 ---- Animal proteins ---- SIN3-HDAC complex-associated factor Source.63: DFBPPR12070 ---- Animal proteins ---- Transmembrane protein 237 Source.64: DFBPPR12415 ---- Animal proteins ---- 72 kDa type IV collagenase Source.65: DFBPPR12881 ---- Animal proteins ---- CX3C chemokine receptor 1 Source.66: DFBPPR13150 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.67: DFBPPR13541 ---- Animal proteins ---- Transforming growth factor beta-1 proprotein Source.68: DFBPPR14866 ---- Marine protein ---- Tyrosine 3-monooxygenase Source.69: DFBPPR7942 ---- Plant protein ---- Polyphenol oxidase A1, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Immunomodulatory activity	The peptide showed immunomodulatory activity.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	All the peptides were synthesized by solid-phase methods, using chloromethylated copolymer of styrene and 1% divinyl benzene.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wieczorek Z, Słoń J, Kluczyk A, Zbozień R, Stafanowicz P, Siemion IZ. The immunomodulatory diversity of the proteins of the transforming growth factor beta (TGF beta) family. Int J Pept Protein Res. 1995 Aug;46(2):113-8. PMID: 8567165 DOI: 10.1111/j.1399-3011.1995.tb01326.x
Other literature(s)	N.D
PubDate	1995