E-mail:gzliang@cqu.edu.cn    Tel: (86)2365102507

List of peptide properties
DFBP ID - DFBPIMMU0098(Immunomodulatory peptide)
DFBP ID DFBPIMMU0098
Peptide sequence PFNQL
Type Native peptide
Peptide/Function name Immunomodulatory peptide
Function-activity relationship
Main bioactivity Immunomodulatory activity
Otheir bioactivity N.D
Calculated physicochemical properties
Three-letter amino acid Pro-Phe-Asn-Gln-Leu
Single-letter amino acid PFNQL
Peptide length 5
Peptide mass
Experimental mass Theoretical mass
618.3 Da 617.70 Da c
Net charge 0.00 c
Isoelectric point (pI) 5.97 c
IC50 N.D
pIC50 N.D
GRAVY -0.4000 c
Hydrophilic residue ratio 60% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Plant
Organism/Source Corn, maize (Zea mays L.)
Precursor protein α-zein
Residue position f(118-122)
Precursor protein(s) search
Source.1: DFBPPR3804 ---- Plant proteins ---- Probable monogalactosyldiacylglycerol synthase 1, chloroplastic
Source.2: DFBPPR5246 ---- Plant proteins ---- Probable monogalactosyldiacylglycerol synthase, chloroplastic
Source.3: DFBPPR5851 ---- Plant proteins ---- 22 kDa alpha-zein 4
Source.4: DFBPPR5868 ---- Plant proteins ---- 22 kDa alpha-zein 16
Source.5: DFBPPR5957 ---- Plant proteins ---- Protein FLOURY 2
Source.6: DFBPPR6029 ---- Plant proteins ---- Zein-alpha PMS2
Source.7: DFBPPR6032 ---- Plant proteins ---- 22 kDa alpha-zein 8b
Source.8: DFBPPR6037 ---- Plant proteins ---- 19 kDa alpha-zein 19C2
Source.9: DFBPPR6041 ---- Plant proteins ---- 22 kDa alpha-zein 14
Source.10: DFBPPR6057 ---- Plant proteins ---- Zein-alpha PMS1
Source.11: DFBPPR6067 ---- Plant proteins ---- 22 kDa alpha-zein 8
Source.12: DFBPPR6080 ---- Plant proteins ---- Zein-alpha 19A2
Source.13: DFBPPR6081 ---- Plant proteins ---- Zein-alpha 19B1
Source.14: DFBPPR6082 ---- Plant proteins ---- Zein-alpha 19D1
Source.15: DFBPPR6085 ---- Plant proteins ---- Zein-alpha A30
Source.16: DFBPPR6088 ---- Plant proteins ---- Zein-alpha ZG99
Source.17: DFBPPR6093 ---- Plant proteins ---- Zein-alpha 19C1
Source.18: DFBPPR6094 ---- Plant proteins ---- Zein-alpha PZ19.1
Source.19: DFBPPR6095 ---- Plant proteins ---- Zein-alpha A20
Source.20: DFBPPR6096 ---- Plant proteins ---- Zein-alpha GZ19AB11
Source.21: DFBPPR6101 ---- Plant proteins ---- Zein-alpha M6
Source.22: DFBPPR6107 ---- Plant proteins ---- 22 kDa alpha-zein 8
Source.23: DFBPPR6110 ---- Plant proteins ---- Zein-alpha Z4
Source.24: DFBPPR6123 ---- Plant proteins ---- 22 kDa alpha-zein 8
Source.25: DFBPPR6127 ---- Plant proteins ---- 22 kDa alpha-zein 14
Source.26: DFBPPR6131 ---- Plant proteins ---- Zein-alpha B49
Source.27: DFBPPR7924 ---- Plant protein ---- Kafirin PSKR2
Link-research
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
Immunomodulatory activity

As shown in Table 2, the peptide PFNQL showed potent IL-6 inhibitory capability (46.2 ± 4.5%) at the concentration of 0.5 mM.

IM1.png
Specific target protein(s) N.D
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N.D
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

The zein and corn germ were prepared at 5% and 10% (w/v) aqueous suspension, respectively, and then hydrolyzed by thermolysin (pH8.0, 0.5% E/S, 60 °C, 3 h), pepsin (pH2.0, 0.5% E/S, 37 °C, 3 h) or a sequential combination of pepsin and thermolysin to produce hydrolysates.

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
BIOPEP-UWM [D1] -
APD [D2] -
BioPepDB [D3] -
MBPDB [D4] -
Reference(s)
Primary literature Liu P , Liao W , Qi X , et al. Identification of immunomodulatory peptides from zein hydrolysates[J]. European Food Research and Technology, 2020, 246(5).
Other literature(s) N.D
PubDate 2020
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214