Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPMIBP0020(Mineral-binding peptide)

DFBP ID	DFBPMIBP0020
Peptide sequence	VYQHQKAMKPWIQPKTKVIPYVRYL
Type	Native peptide
Peptide/Function name	Mineral-binding petpide, Calmodulin-binding peptide

Function-activity relationship

Main bioactivity	Mineral-binding activity
Otheir bioactivity	Antimicrobial activity ^[D1], Haemolytic activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Val-Tyr-Gln-His-Gln-Lys-Ala-Met-Lys-Pro-Trp-Ile-Gln-Pro-Lys-Thr-Lys-Val-Ile-Pro-Tyr-Val-Arg-Tyr-Leu

Single-letter amino acid

VYQHQKAMKPWIQPKTKVIPYVRYL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	3115.77 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.48 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6000^c

Hydrophilic residue ratio

48%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	α_s2-Casein
Residue position	f(183-207)
Precursor protein(s) search	Source.1: DFBPPR8494 ---- Milk proteins ---- Alpha-S2-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Mineral-binding activity

Fig. 1 shows the effects of α_s2-casein (164-179), α_s2-casein (183-206) and α_s2-casein (183-207) on Calmodulin-induced activation of 3',5'-cyclic-nucleotide phosphodiesterase (PDE, EC 3.1.4.17). The approximate IC₅₀ values of these peptides were 38, 6.9 and 1.1 μM respectively. The concentration of each peptide that totally inhibited the activation of PDE induced by Calmodulin had no significant effect on the basal PDE activity.

Fig. 1. Effects of the peptides derived from α-casein on the calmodulin-indueed phosphodiesterase activation. Effects of various concentrations of ○, α_s2-casein (164-179); △, α_s2-casein (183-206) and □, α_s2-casein (183-207) are shown. Each point is the mean of duplicate determinations.

Specific target protein(s)

Specific Target Protein(s):
3',5'-cyclic-nucleotide phosphodiesterase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Peptides that inhibit calmodulin-dependent cyclic nucleotide phosphodiesterase were isolated from a pepsin digest of α-casein.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

These results demonstrated that the affinities of these peptides for calmodulin are comparable to the affinities of certain endogenous neurohormones and proteins that interact with calmodulin.

Database cross-references

DFBP

[D1]	DFBPAMIC0197
[D2]	DFBPHAPE0019
[D3]	DFBPMUFU0704

BIOPEP-UWM

[D4]

3034, 3964, 5469

APD

[D5]

BioPepDB

[D6]

MBPDB

[D7]

Reference(s)

Primary literature	Kizawa K, Naganuma K, Murakami U. Calmodulin-binding peptides isolated from alpha-casein peptone. J Dairy Res. 1995 Nov;62(4):587-92. PMID: 8568029 DOI: 10.1017/s0022029900031319
Other literature(s)	N.D
PubDate	1995