Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0005(Neuropeptide peptide)

DFBP ID	DFBPNEUR0005
Peptide sequence	LPLRF
Type	Native peptide
Peptide/Function name	Neuropeptide

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Pro-Leu-Arg-Phe

Single-letter amino acid

LPLRF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	644.80 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.8600^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Chicken (Gallus)
Precursor protein	Brain protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR16069 ---- Animal proteins ---- T-cell surface glycoprotein CD4 Source.2: DFBPPR18866 ---- Animal proteins ---- Autophagy-related protein 9A Source.3: DFBPPR19463 ---- Animal proteins ---- Pro-FMRFamide-related neuropeptide VF Source.4: DFBPPR22035 ---- Animal proteins ---- Protein CCSMST1 Source.5: DFBPPR10106 ---- Animal proteins ---- Glycerol-3-phosphate acyltransferase 3 Source.6: DFBPPR10475 ---- Animal proteins ---- Adenylate cyclase type 9 Source.7: DFBPPR12003 ---- Animal proteins ---- FMRFamide-like neuropeptide Source.8: DFBPPR13814 ---- Animal proteins ---- Pro-FMRFamide-related neuropeptide VF Source.9: DFBPPR14113 ---- Marine protein ---- G-protein coupled receptor 183
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity	Peptide LPLRF showed neuromodulatory activity (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	Chicken brains were extracted by boiling in water, followed by acidification with acetic acid to 0.5 M. The extracts were centrifuged and supernatants concentrated by adsorption to C18 Sep-pak cartridges (Waters Associates), elution with acetonitrile and lyophilization.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Dockray GJ, Reeve JR Jr, Shively J, Gayton RJ, Barnard CS. A novel active pentapeptide from chicken brain identified by antibodies to FMRFamide. Nature. 1983 Sep 22-28;305(5932):328-30. PMID: 6137771 DOI: 10.1038/305328a0
Other literature(s)	[1] Gaus G, Doble KE, Price DA, Greenberg MJ, Lee TD, Battelle BA. The Sequences of Five Neuropeptides Isolated from Limulus using Antisera to FMRFamide. Biol Bull. 1993 Jun;184(3):322-329.
PubDate	1983