Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0006(Neuropeptide peptide)

DFBP ID	DFBPNEUR0006
Peptide sequence	KRNKNNIA
Type	Native peptide
Peptide/Function name	Neuropeptide, Glucagon-37 (bioactive enteroglucagon/oxyntomodulin)

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala

Single-letter amino acid

KRNKNNIA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	957.09 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.68 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.0625^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Pig
Precursor protein	Porcine jejuno-ileum
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR16110 ---- Animal proteins ---- Pro-glucagon Source.2: DFBPPR17133 ---- Animal proteins ---- Pro-glucagon Source.3: DFBPPR17134 ---- Animal proteins ---- Pro-glucagon Source.4: DFBPPR8589 ---- Animal proteins ---- Pro-glucagon Source.5: DFBPPR13709 ---- Animal proteins ---- Pro-glucagon
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity	The potency of peptide Glucagon-37 relative to pancreatic glucagon in interacting with an antiglucagon antibody, hepatic glucagon‐binding sites and hepatic adenylate cyclase was ∼100%, 20% and 10%, respectively. In contrast, it is ∼20‐times more potent than glucagon in oxyntic glands, justifying the term 'oxyntomodulin'.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptides were hydrolyzed with two different enzymes: Armillaria melleaprotease(a gift from Dr P.L. Walton, ICI Laboratories, Cheshire) and TLCK-treated α-chymotrypsin (EC 3.4.21.1) from Worthington (USA).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Bataille D, Tatemoto K, Gespach C, Jörnvall H, Rosselin G, Mutt V. Isolation of glucagon-37 (bioactive enteroglucagon/oxyntomodulin) from porcine jejuno-ileum. Characterization of the peptide. FEBS Lett. 1982 Sep 6;146(1):79-86. PMID: 7140977 DOI: 10.1016/0014-5793(82)80709-6
Other literature(s)	N.D
PubDate	1982