Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0009(Neuropeptide peptide)

DFBP ID	DFBPNEUR0009
Peptide sequence	HLSPFSPRL
Type	Native peptide
Peptide/Function name	Neuropeptide, Pyrokinin, Tenmo-PK-3

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

His-Leu-Ser-Pro-Phe-Ser-Pro-Arg-Leu

Single-letter amino acid

HLSPFSPRL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
1026.54 Da	1053.21 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.2333^c

Hydrophilic residue ratio

55.56%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Insect
Organism/Source	Mealworm beetle, Tenebrio molitor
Precursor protein	Brain and corpora cardiaca-corpora allata (CC-CA) protein
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity	Peptide HLSPFSPRLamide showed neuromodulatory activity (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	Brains (one hundred, mixed sex) and (separately) corpora cardiaca-corpora allata (CC-CA) (25 gland pairs for each sex) were dissected from adult T. molitor and placed into Eppendorf tubes containing ice-cold 100% methanol. All tissues were infused at room temperature for 15–30 min, and then removed and subsequently re-extracted in 0.1% (v/v) trifluoroacetic acid (TFA).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Weaver RJ, Audsley N. Neuropeptides of the beetle, Tenebrio molitor identified using MALDI-TOF mass spectrometry and deduced sequences from the Tribolium castaneum genome. Peptides. 2008 Feb;29(2):168-78. PMID: 18201799 DOI: 10.1016/j.peptides.2007.09.020
Other literature(s)	N.D
PubDate	2008