DFBP ID - DFBPNEUR0077(Neuropeptide peptide)

DFBP ID DFBPNEUR0077 Peptide sequence DRNFLRF Type Native peptide Peptide/Function name Neuropeptide, Mar-FLP1

Function-activity relationship

Main bioactivity Neuropeptide activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Asp-Arg-Asn-Phe-Leu-Arg-Phe Single-letter amino acid DRNFLRF Peptide length 7 Peptide mass Experimental mass Theoretical mass 965.70 Da 967.08 Da c Net charge 0.00 c Isoelectric point (pI) 10.88 c IC50 N.D pIC50 N.D GRAVY -0.9429 c Hydrophilic residue ratio 42.86% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal Organism/Source Giant freshwater prawn (Macrobrachium rosenbergii) Precursor protein Eyestalk protein Residue position N.D Precursor protein(s) search Source.1: DFBPPR14836 ---- Marine protein ---- FMRFamide-like neuropeptide 3 Link-research Inductive analysis Link 1: DFBPNEUR0101----Crayfish, Procambarus clarkii----Pericardial organ extract protein

Biological/Functional activity & target protein

Neuropeptide activity The peptide DRNFLRFamide has neuropeptide activity as neurotransmitters or neuromodulators. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools No prediction can be made about the peptide bitterness. prediction SMILES N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation Methanol extraction Enzyme(s)/starter culture Freeze powder of the eyestalks was prepared by grinding the eyestalks in a mortar containing dry ice; powdered eyestalks were then extracted in methanol/acetic acid/H2O (90:1:9, v/v) (0.5 ml eyestalk−1) for 24 h at 4°C.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 2895

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Sithigorngul, P., Saraithongkum, W., Jaideechoey, S., Longyant, S., Sithigorngul, W. Novel FMRFamide-like neuropeptides from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 1998, 120, 587-95. DOI: 10.1016/s0305-0491(98)10051-2 Other literature(s) [1] Sithigorngul P, Saraithongkum W, Longyant S, Panchan N, Sithigorngul W, Petsom A. Three more novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Peptides. 2001 Feb;22(2):191-7. PubDate 1998