Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0083(Neuropeptide peptide)

DFBP ID	DFBPNEUR0083
Peptide sequence	TNRNFLRF
Type	Native peptide
Peptide/Function name	Neuropeptide, FLI 4

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Thr-Asn-Arg-Asn-Phe-Leu-Arg-Phe

Single-letter amino acid

TNRNFLRF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1067.20 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

12.50 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9125^c

Hydrophilic residue ratio

37.5%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Homarus americanus
Precursor protein	Pericardial organ protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR14823 ---- Marine protein ---- FMRFamide-like neuropeptide 4
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity	The pericardial organs release FMRFamide like immunoreactivity (FLI) when depolarized with 100 mM K⁺ in the presence of calcium. Between 75 and 80% of this release is accounted for by FLI 4 (TNRNFLRFamide). Preliminary studies show that this peptide can act as a modulator of exoskeletal and cardiac neuromuscular junctions.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Crude methanolic extracts of pericardial organs containing approximately 300 fmol (FMRFamide equivalents) of FMRFamidelike immunoreactivity were incubated for 1hour at 37°C with 0.05 mg trypsin (type 1 from bovine pancreas-Sigma) in sodium phosphate buffer (pH 7.5).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide was also found in Blue crab, Callinectes sapidus (Relative potency: 22.4 ± 13.6, Relative efficacy: 1.25) ^[3].

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Trimmer BA, Kobierski LA, Kravitz EA. Purification and characterization of FMRFamidelike immunoreactive substances from the lobster nervous system: isolation and sequence analysis of two closely related peptides. J Comp Neurol. 1987 Dec 1;266(1):16-26. PMID: 3429714 DOI: 10.1002/cne.902660103
Other literature(s)	[1] Sithigorngul P, Saraithongkum W, Longyant S, Panchan N, Sithigorngul W, Petsom A. Three more novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Peptides. 2001 Feb;22(2):191-7. [2] Sithigorngul, P., Saraithongkum, W., Jaideechoey, S., Longyant, S., Sithigorngul, W. Novel FMRFamide-like neuropeptides from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 1998, 120, 587-95. [3] Krajniak KG. The identification and structure-activity relations of a cardioactive FMRFamide-related peptide from the blue crab Callinectes sapidus. Peptides. 1991 Nov-Dec;12(6):1295-302.
PubDate	1987