Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0084(Neuropeptide peptide)

DFBP ID	DFBPNEUR0084
Peptide sequence	GYNRSFLRF
Type	Native peptide
Peptide/Function name	Neuropeptide, FaRP

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Tyr-Asn-Arg-Ser-Phe-Leu-Arg-Phe

Single-letter amino acid

GYNRSFLRF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	1159.30 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.20 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6222^c

Hydrophilic residue ratio

44.44%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Blue crab, Callinectes sapidus
Precursor protein	Pericardial organs and thoracic ganglia extract protein
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity

The peptide GYNRSFLRFamide caused a dose-dependent increase in heart rate; threshold was 10 to 30 nM, and the EC₅₀ was 323 ± 62 nM (Relative potency: 1.00, Relative efficacy: 1.00). A structure-activity study of GYNRSFLRFamide on the crab heart suggests that, for full potency, a peptide should be at least a heptapeptide with the sequence XXZFLRFamide, where X is any amino acid and Z is either asparagine or serine.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

NCC(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	The pericardial organs and thoracic ganglia of the blue crab Callinectes sapidus were resected and extracted. The extracts were fractionated by HPLC and the fractions analyzed by a radioimmunoassay (RIA) to FMRFamide.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Krajniak KG. The identification and structure-activity relations of a cardioactive FMRFamide-related peptide from the blue crab Callinectes sapidus. Peptides. 1991 Nov-Dec;12(6):1295-302. PMID: 1815216 DOI: 10.1016/0196-9781(91)90210-g
Other literature(s)	[1] Sithigorngul P, Saraithongkum W, Longyant S, Panchan N, Sithigorngul W, Petsom A. Three more novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Peptides. 2001 Feb;22(2):191-7. [2] Sithigorngul, P., Saraithongkum, W., Jaideechoey, S., Longyant, S., Sithigorngul, W. Novel FMRFamide-like neuropeptides from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 1998, 120, 587-95.
PubDate	1991