Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0089(Neuropeptide peptide)

DFBP ID	DFBPNEUR0089
Peptide sequence	ENFLRF
Type	Native peptide
Peptide/Function name	Neuropeptide

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Asn-Phe-Leu-Arg-Phe

Single-letter amino acid

ENFLRF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	824.92 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.95 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.3500^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Blue crab, Callinectes sapidus
Precursor protein	Pericardial organs and thoracic ganglia extract protein
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity	The peptide ENFLRFamide showed neuropeptide activity (Relative potency: 0.48 ± 0.14, Relative efficacy: 0.80).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	The pericardial organs and thoracic ganglia of the blue crab Callinectes sapidus were resected and extracted. The extracts were fractionated by HPLC and the fractions analyzed by a radioimmunoassay (RIA) to FMRFamide.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Krajniak KG. The identification and structure-activity relations of a cardioactive FMRFamide-related peptide from the blue crab Callinectes sapidus. Peptides. 1991 Nov-Dec;12(6):1295-302. PMID: 1815216 DOI: 10.1016/0196-9781(91)90210-g
Other literature(s)	N.D
PubDate	1991