Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0094(Neuropeptide peptide)

DFBP ID	DFBPNEUR0094
Peptide sequence	FLRF
Type	Native peptide
Peptide/Function name	Neuropeptide

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Leu-Arg-Phe

Single-letter amino acid

FLRF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	581.70 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

1.2250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Blue crab, Callinectes sapidus
Precursor protein	Pericardial organ/thoracic ganglion extract protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0822 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC4 Source.2: DFBPPR0829 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC5 Source.3: DFBPPR0830 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC6 Source.4: DFBPPR0927 ---- Plant proteins ---- Serine/threonine-protein kinase TOR Source.5: DFBPPR1068 ---- Plant proteins ---- E3 ubiquitin-protein ligase DIS1 Source.6: DFBPPR1144 ---- Plant proteins ---- Meiotic recombination protein SPO11-4 Source.7: DFBPPR2039 ---- Plant proteins ---- Protein MONOCULM 1 Source.8: DFBPPR2406 ---- Plant proteins ---- Probable serine/threonine-protein kinase WNK6 Source.9: DFBPPR2605 ---- Plant proteins ---- Clathrin light chain 2 Source.10: DFBPPR3602 ---- Plant proteins ---- Coatomer subunit alpha-2 Source.11: DFBPPR3822 ---- Plant proteins ---- Coatomer subunit alpha-3 Source.12: DFBPPR3884 ---- Plant proteins ---- Casparian strip membrane protein 4 Source.13: DFBPPR3928 ---- Plant proteins ---- Coatomer subunit alpha-1 Source.14: DFBPPR3940 ---- Plant proteins ---- Putative cyclin-D2-3 Source.15: DFBPPR4104 ---- Plant proteins ---- Casparian strip membrane protein 5 Source.16: DFBPPR4114 ---- Plant proteins ---- SPX domain-containing membrane protein Os06g0129400 Source.17: DFBPPR4189 ---- Plant proteins ---- Photosystem I assembly protein Ycf4 Source.18: DFBPPR4243 ---- Plant proteins ---- UDP-glucose:2-hydroxyflavanone C-glucosyltransferase Source.19: DFBPPR4267 ---- Plant proteins ---- Putative cyclin-D7-1 Source.20: DFBPPR4582 ---- Plant proteins ---- Probable calcium-binding protein CML12 Source.21: DFBPPR4656 ---- Plant proteins ---- SPX domain-containing membrane protein Os09g0521800 Source.22: DFBPPR4810 ---- Plant proteins ---- Hydrophobic protein OSR8 Source.23: DFBPPR5825 ---- Plant proteins ---- UDP-glycosyltransferase 708A6 Source.24: DFBPPR5950 ---- Plant proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.25: DFBPPR6278 ---- Plant proteins ---- Protein TIC 55, chloroplastic Source.26: DFBPPR6664 ---- Plant proteins ---- Aluminum-activated malate transporter 1 Source.27: DFBPPR6775 ---- Plant proteins ---- NADH-ubiquinone oxidoreductase chain 4 Source.28: DFBPPR6876 ---- Plant proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.29: DFBPPR6950 ---- Plant proteins ---- Photosystem I assembly protein Ycf4 Source.30: DFBPPR7316 ---- Plant proteins ---- Photosystem I assembly protein Ycf4 Source.31: DFBPPR16128 ---- Animal proteins ---- Cubilin Source.32: DFBPPR16201 ---- Animal proteins ---- X-linked retinitis pigmentosa GTPase regulator Source.33: DFBPPR17066 ---- Animal proteins ---- 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Source.34: DFBPPR17422 ---- Animal proteins ---- Rhodopsin kinase GRK1 Source.35: DFBPPR17516 ---- Animal proteins ---- Steroid 21-hydroxylase Source.36: DFBPPR17874 ---- Animal proteins ---- Endonuclease 8-like 2 Source.37: DFBPPR17921 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 2 Source.38: DFBPPR18161 ---- Animal proteins ---- Disintegrin and metalloproteinase domain-containing protein 2 Source.39: DFBPPR18165 ---- Animal proteins ---- Retinaldehyde-binding protein 1 Source.40: DFBPPR18461 ---- Animal proteins ---- Glutamine--tRNA ligase Source.41: DFBPPR18605 ---- Animal proteins ---- Mitochondrial proton/calcium exchanger protein Source.42: DFBPPR18961 ---- Animal proteins ---- Transmembrane protein 184A Source.43: DFBPPR18990 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform Source.44: DFBPPR19701 ---- Animal proteins ---- TATA box-binding protein-associated factor RNA polymerase I subunit B Source.45: DFBPPR20048 ---- Animal proteins ---- Ubiquitin conjugation factor E4 A Source.46: DFBPPR20154 ---- Animal proteins ---- NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Source.47: DFBPPR20280 ---- Animal proteins ---- Sodium-dependent phosphate transporter 2 Source.48: DFBPPR20440 ---- Animal proteins ---- 28S ribosomal protein S25, mitochondrial Source.49: DFBPPR20853 ---- Animal proteins ---- Hemopexin Source.50: DFBPPR20968 ---- Animal proteins ---- Ras GTPase-activating protein 3 Source.51: DFBPPR20975 ---- Animal proteins ---- 39S ribosomal protein L2, mitochondrial Source.52: DFBPPR21316 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit alpha Source.53: DFBPPR21478 ---- Animal proteins ---- FTS and Hook-interacting protein Source.54: DFBPPR21907 ---- Animal proteins ---- Molybdate-anion transporter Source.55: DFBPPR22233 ---- Animal proteins ---- RNA exonuclease 5 Source.56: DFBPPR22301 ---- Animal proteins ---- Transmembrane protein 184B Source.57: DFBPPR22762 ---- Animal proteins ---- Uncharacterized protein C6orf136 homolog Source.58: DFBPPR8569 ---- Animal proteins ---- Ryanodine receptor 1 Source.59: DFBPPR8679 ---- Animal proteins ---- Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 Source.60: DFBPPR9093 ---- Animal proteins ---- Hemopexin Source.61: DFBPPR9196 ---- Animal proteins ---- Steroid 21-hydroxylase Source.62: DFBPPR9743 ---- Animal proteins ---- SLA class II histocompatibility antigen, DQ haplotype D beta chain Source.63: DFBPPR9954 ---- Animal proteins ---- Tolloid-like protein 1 Source.64: DFBPPR10249 ---- Animal proteins ---- Heparan sulfate 2-O-sulfotransferase 1 Source.65: DFBPPR10337 ---- Animal proteins ---- Alpha-tectorin Source.66: DFBPPR10401 ---- Animal proteins ---- Heparanase Source.67: DFBPPR10513 ---- Animal proteins ---- Parafibromin Source.68: DFBPPR10648 ---- Animal proteins ---- PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Source.69: DFBPPR10860 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 2 Source.70: DFBPPR11044 ---- Animal proteins ---- Mitochondrial proton/calcium exchanger protein Source.71: DFBPPR11238 ---- Animal proteins ---- Retinaldehyde-binding protein 1 Source.72: DFBPPR11402 ---- Animal proteins ---- Contactin-associated protein-like 5 Source.73: DFBPPR12069 ---- Animal proteins ---- Transmembrane channel-like protein 7 Source.74: DFBPPR12254 ---- Animal proteins ---- Cytochrome P450 1A2 Source.75: DFBPPR12275 ---- Animal proteins ---- Ryanodine receptor 3 Source.76: DFBPPR12281 ---- Animal proteins ---- Ryanodine receptor 1 Source.77: DFBPPR12290 ---- Animal proteins ---- Ryanodine receptor 2 Source.78: DFBPPR12469 ---- Animal proteins ---- Hemopexin Source.79: DFBPPR12718 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform Source.80: DFBPPR12763 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Source.81: DFBPPR12973 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform Source.82: DFBPPR14823 ---- Marine protein ---- FMRFamide-like neuropeptide 4 Source.83: DFBPPR14836 ---- Marine protein ---- FMRFamide-like neuropeptide 3 Source.84: DFBPPR15317 ---- Microorganism protein ---- Phosphatidylinositol transfer protein SFH5 Source.85: DFBPPR7873 ---- Plant protein ---- Casparian strip membrane protein 4 Source.86: DFBPPR8335 ---- Plant protein ---- Photosystem I assembly protein Ycf4
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity	The peptide FLRFamide showed neuropeptide activity (Relative potency: 0.06 ± 0.01, Relative efficacy: 0.85).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	The pericardial organs and thoracic ganglia of the blue crab Callinectes sapidus were resected and extracted. The extracts were fractionated by HPLC and the fractions analyzed by a radioimmunoassay (RIA) to FMRFamide.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Krajniak KG. The identification and structure-activity relations of a cardioactive FMRFamide-related peptide from the blue crab Callinectes sapidus. Peptides. 1991 Nov-Dec;12(6):1295-302. PMID: 1815216 DOI: 10.1016/0196-9781(91)90210-g
Other literature(s)	N.D
PubDate	1991