Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0095(Neuropeptide peptide)

DFBP ID	DFBPNEUR0095
Peptide sequence	FMRF
Type	Native peptide
Peptide/Function name	Neuropeptide

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Phe-Met-Arg-Phe

Single-letter amino acid

FMRF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	599.74 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.7500^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Blue crab, Callinectes sapidus
Precursor protein	Pericardial organs and thoracic ganglia extract protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR3341 ---- Plant proteins ---- Transcriptional adapter ADA2 Source.2: DFBPPR4115 ---- Plant proteins ---- Cyclin-B1-2 Source.3: DFBPPR4358 ---- Plant proteins ---- Photosystem II reaction center PSB28 protein, chloroplastic Source.4: DFBPPR16933 ---- Animal proteins ---- Proenkephalin-A Source.5: DFBPPR18801 ---- Animal proteins ---- Mitochondrial pyruvate carrier 1 Source.6: DFBPPR20944 ---- Animal proteins ---- Pikachurin Source.7: DFBPPR22308 ---- Animal proteins ---- Mitochondrial pyruvate carrier-like protein Source.8: DFBPPR8794 ---- Animal proteins ---- NPC intracellular cholesterol transporter 1 Source.9: DFBPPR11707 ---- Animal proteins ---- Nuclear cap-binding protein subunit 3 Source.10: DFBPPR14473 ---- Marine protein ---- Photosystem II reaction center Psb28 protein Source.11: DFBPPR15144 ---- Microorganism protein ---- Palmitoyltransferase PFA4 Source.12: DFBPPR8120 ---- Plant protein ---- Protein TIC 214
Link-research Inductive analysis	Link 1: DFBPNEUR0102----Clam Macrocallista nimbosa----Ganglia cardioexcitatory substance

Biological/Functional activity & target protein

Neuropeptide activity	The peptide FMRFamide showed neuropeptide activity (Relative potency: 0.02 ± 0.01, Relative efficacy: 0.86).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	The pericardial organs and thoracic ganglia of the blue crab Callinectes sapidus were resected and extracted. The extracts were fractionated by HPLC and the fractions analyzed by a radioimmunoassay (RIA) to FMRFamide.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Krajniak KG. The identification and structure-activity relations of a cardioactive FMRFamide-related peptide from the blue crab Callinectes sapidus. Peptides. 1991 Nov-Dec;12(6):1295-302. PMID: 1815216 DOI: 10.1016/0196-9781(91)90210-g
Other literature(s)	[1] Sithigorngul P, Saraithongkum W, Longyant S, Panchan N, Sithigorngul W, Petsom A. Three more novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Peptides. 2001 Feb;22(2):191-7.
PubDate	1991