DFBP ID - DFBPNEUR0100(Neuropeptide peptide)

DFBP ID DFBPNEUR0100 Peptide sequence NRNFLRF Type Native peptide Peptide/Function name Neuropeptide, NF1

Function-activity relationship

Main bioactivity Neuropeptide activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Asn-Arg-Asn-Phe-Leu-Arg-Phe Single-letter amino acid NRNFLRF Peptide length 7 Peptide mass Experimental mass Theoretical mass N.D 966.10 Da c Net charge 0.00 c Isoelectric point (pI) 12.50 c IC50 N.D pIC50 N.D GRAVY -0.9429 c Hydrophilic residue ratio 42.86% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Animal, Marine Organism/Source Crayfish, Procambarus clarkii Precursor protein Pericardial organ extract protein Residue position N.D Precursor protein(s) search Source.1: DFBPPR14823 ---- Marine protein ---- FMRFamide-like neuropeptide 4 Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity NF1 (NRNFLRFamide) was cardioexcitatory and augment synaptic transmission at a neuromuscular synapse, and evidence is presented for the calcium-dependent release of this peptide from the pericardial organs. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools No prediction can be made about the peptide bitterness. prediction SMILES N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation Extraction Enzyme(s)/starter culture Pericardial organs of the crayfish, Procambarus clarkii, were removed, and material from them was extracted and fractionated using two sequential reverse-phase HPLC columns.

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 2894

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Mercier AJ, Orchard I, TeBrugge V, Skerrett M. Isolation of two FMRFamide-related peptides from crayfish pericardial organs. Peptides. 1993 Mar-Apr;14(2):137-43. PMID: 8387183 DOI: 10.1016/0196-9781(93)90021-8 Other literature(s) [1] Sithigorngul, P., Saraithongkum, W., Jaideechoey, S., Longyant, S., Sithigorngul, W. Novel FMRFamide-like neuropeptides from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 1998, 120, 587-95. [2] Sithigorngul P, Saraithongkum W, Longyant S, Panchan N, Sithigorngul W, Petsom A. Three more novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Peptides. 2001 Feb;22(2):191-7. PubDate 1993