Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPNEUR0103(Neuropeptide peptide)

DFBP ID	DFBPNEUR0103
Peptide sequence	GGRSPSLRLRF
Type	Native peptide
Peptide/Function name	Neuropeptide, Lip-HP

Function-activity relationship

Main bioactivity	Neuropeptide activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Gly-Arg-Ser-Pro-Ser-Leu-Arg-Leu-Arg-Phe

Single-letter amino acid

GGRSPSLRLRF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
1244.60 Da	1245.43 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

12.80 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.6455^c

Hydrophilic residue ratio

54.55%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Horseshoe crab Limulus polyphemus
Precursor protein	Brains (including both the protocerebrum and the circumesophageal ring)
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Neuropeptide activity	Concentrations of synthetic GGRSPSLRLRFamide between 2 × 10^-8 and 10^-6 mol/l had no effect on the rate or amplitude of isolated Limulus hearts. A slight decrease in amplitude was finally observed at 5 × 10^-6 mol/l, but no dose-response curve could be established for this peptide.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)NCC(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	Brains (including both the protocerebrum and the circumesophageal ring) were collected from horseshoe crabs over several months as the animals were used for visual system studies. The acetone extracts were decanted from the pieces of tissue, then clarified by centrifugation, and reduced in volume on a rotary evaporator.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Gaus G, Doble KE, Price DA, Greenberg MJ, Lee TD, Battelle BA. The Sequences of Five Neuropeptides Isolated from Limulus using Antisera to FMRFamide. Biol Bull. 1993 Jun;184(3):322-329. PMID: 29300545 DOI: 10.2307/1542450
Other literature(s)	[1] Sithigorngul, P., Saraithongkum, W., Jaideechoey, S., Longyant, S., Sithigorngul, W. Novel FMRFamide-like neuropeptides from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 1998, 120, 587-95. [2] Sithigorngul P, Saraithongkum W, Longyant S, Panchan N, Sithigorngul W, Petsom A. Three more novel FMRFamide-like neuropeptide sequences from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii. Peptides. 2001 Feb;22(2):191-7.
PubDate	1993