Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0013(Opioid peptide)

DFBP ID	DFBPOPIO0013
Peptide sequence	EMPFPK
Type	Native peptide
Peptide/Function name	Opioid peptide

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Glu-Met-Pro-Phe-Pro-Lys

Single-letter amino acid

EMPFPK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	747.90 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9833^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	γ-Casein
Residue position	f(108-113)
Precursor protein(s) search	Source.1: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.3: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity	The EMPFPK peptide displayed a selective potentiating activity on isolated guinea pig ileum (u-opiate receptor) for bradykinin (1.3 nM) and showed an in vitro competitive inhibitor effect on angiotensin-converting enzyme and presented an opiate-like activity as opioid agonist.
Specific target protein(s)	Specific Target Protein(s): Mu-type opioid receptor

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCSC)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with Trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0558, DFBPACEI1158
[D2]	DFBPMUFU0135

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Perpetuo EA, Juliano L, Lebrun I. Biochemical and pharmacological aspects of two bradykinin-potentiating peptides obtained from tryptic hydrolysis of casein. J Protein Chem. 2003 Nov;22(7-8):601-6. PMID: 14714726 DOI: 10.1023/b:jopc.0000008724.98339.ff
Other literature(s)	[1] Park Y W. Bioactive Components in Milk and Dairy Products[M]. 2009.
PubDate	2003