Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0014(Opioid peptide)

DFBP ID	DFBPOPIO0014
Peptide sequence	YPSYGLNY
Type	Native peptide
Peptide/Function name	Opioid peptide, Casoxin A

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Ser-Tyr-Gly-Leu-Asn-Tyr

Single-letter amino acid

YPSYGLNY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	976.04 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.83 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.8000^c

Hydrophilic residue ratio

37.5%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein^[2]
Precursor protein	κ-Casein
Residue position	f(35-42)
Precursor protein(s) search	Source.1: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.2: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.3: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.4: DFBPPR8492 ---- Milk proteins ---- Kappa-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity	Opioid antagonist, this peptide showed a relatively low opioid antagonistic activity in comparison to the esterified peptides (IC₅₀ = 400.0 umol/l) and has been characterized as μ-type ligands^[2].
Specific target protein(s)	Specific Target Protein(s): Mu-type opioid receptor

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yoshikawa, M. Casoxin D, an opioid antagonist/ileum-contracting/vasorelaxing peptide derived from human alphas1-casein. 1994, pp. 43-8.
Other literature(s)	[1] Park Y W. Bioactive Components in Milk and Dairy Products[M]. 2009. [2] Meisel H, Fitzgerald R J. Opioid peptides encrypted in intact milk protein sequences.[J]. British Journal of Nutrition, 2000, 84(S1):27-31. [3] Kostyra E, Sienkiewiczszłapka E, Jarmołowska B, et al. Opioid peptides derived from milk proteins.[J]. Polish Journal of Food & Nutrition Sciences, 2004. [4] Meisel H. Overview on milk protein-derived peptides.[J]. International Dairy Journal, 1998, 8(5–6):363-373.
PubDate	1994