Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0066(Opioid peptide)

DFBP ID	DFBPOPIO0066
Peptide sequence	YPLG
Type	Native peptide
Peptide/Function name	Opioid peptide, Gliadin 2 exorphin

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Leu-Gly

Single-letter amino acid

YPLG

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	448.51 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.1250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Plant
Organism/Source	Milk, Wheat
Precursor protein	Milk and wheat proteins
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0379 ---- Plant protein ---- Alpha-gliadin Source.2: DFBPPR0380 ---- Plant protein ---- Alpha-gliadin Source.3: DFBPPR0381 ---- Plant protein ---- Alpha-gliadin Source.4: DFBPPR0384 ---- Plant protein ---- Alpha-gliadin Source.5: DFBPPR0389 ---- Plant protein ---- Alpha-gliadin Source.6: DFBPPR1595 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B Source.7: DFBPPR1602 ---- Plant proteins ---- SNW/SKI-interacting protein A Source.8: DFBPPR1605 ---- Plant proteins ---- Phytochrome B Source.9: DFBPPR1614 ---- Plant proteins ---- Bisdemethoxycurcumin synthase Source.10: DFBPPR1652 ---- Plant proteins ---- Photosystem II D2 protein Source.11: DFBPPR1657 ---- Plant proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A Source.12: DFBPPR1662 ---- Plant proteins ---- Probable allantoate deiminase Source.13: DFBPPR3218 ---- Plant proteins ---- Probable lipoxygenase 6 Source.14: DFBPPR3648 ---- Plant proteins ---- Putative lipoxygenase 5 Source.15: DFBPPR5063 ---- Plant proteins ---- Photosystem II D2 protein Source.16: DFBPPR5074 ---- Plant proteins ---- Phytochrome B Source.17: DFBPPR5477 ---- Plant proteins ---- Photosystem II D2 protein Source.18: DFBPPR6232 ---- Plant proteins ---- Photosystem II D2 protein Source.19: DFBPPR6710 ---- Plant proteins ---- Photosystem II D2 protein Source.20: DFBPPR6930 ---- Plant proteins ---- Alpha/beta-gliadin Source.21: DFBPPR6941 ---- Plant proteins ---- Alpha/beta-gliadin A-I Source.22: DFBPPR6954 ---- Plant proteins ---- Alpha/beta-gliadin clone PTO-A10 Source.23: DFBPPR7054 ---- Plant proteins ---- Photosystem II D2 protein Source.24: DFBPPR8453 ---- Plant proteins ---- Photosystem II D2 protein Source.25: DFBPPR8503 ---- Milk proteins ---- Lipoprotein lipase Source.26: DFBPPR15942 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B Source.27: DFBPPR16111 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A Source.28: DFBPPR17691 ---- Animal proteins ---- Integrin alpha-L Source.29: DFBPPR18706 ---- Animal proteins ---- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A Source.30: DFBPPR18913 ---- Animal proteins ---- NLR family CARD domain-containing protein 4 Source.31: DFBPPR20109 ---- Animal proteins ---- Ovarian cancer G-protein coupled receptor 1 Source.32: DFBPPR20389 ---- Animal proteins ---- Leucine-rich repeat-containing protein 7 Source.33: DFBPPR20620 ---- Animal proteins ---- GDP-D-glucose phosphorylase 1 Source.34: DFBPPR20684 ---- Animal proteins ---- Doublesex- and mab-3-related transcription factor A2 Source.35: DFBPPR20788 ---- Animal proteins ---- MICOS complex subunit MIC27 Source.36: DFBPPR21506 ---- Animal proteins ---- Origin recognition complex subunit 3 Source.37: DFBPPR9298 ---- Animal proteins ---- Melanocortin receptor 4 Source.38: DFBPPR9884 ---- Animal proteins ---- Cartilage intermediate layer protein 1 Source.39: DFBPPR9947 ---- Animal proteins ---- Hypothalamic tetradecapeptide Source.40: DFBPPR10358 ---- Animal proteins ---- Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase Source.41: DFBPPR11114 ---- Animal proteins ---- E3 ubiquitin-protein ligase listerin Source.42: DFBPPR11200 ---- Animal proteins ---- Homeobox protein HMX1 Source.43: DFBPPR11641 ---- Animal proteins ---- MICOS complex subunit MIC27 Source.44: DFBPPR12041 ---- Animal proteins ---- Paladin Source.45: DFBPPR13568 ---- Animal proteins ---- Lipoprotein lipase Source.46: DFBPPR13733 ---- Animal proteins ---- Keratin-associated protein 8-1 Source.47: DFBPPR13781 ---- Animal proteins ---- Protein-arginine deiminase type-3 Source.48: DFBPPR14291 ---- Marine protein ---- Photosystem II D2 protein Source.49: DFBPPR14338 ---- Marine protein ---- Photosystem II D2 protein Source.50: DFBPPR15487 ---- Microorganism protein ---- Restriction of telomere capping protein 1 Source.51: DFBPPR7775 ---- Plant protein ---- Photosystem II D2 protein Source.52: DFBPPR7802 ---- Plant protein ---- Phytochrome B Source.53: DFBPPR8050 ---- Plant protein ---- Photosystem II D2 protein Source.54: DFBPPR8226 ---- Plant protein ---- Photosystem II D2 protein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity

The effect of YPLG (0.25 mg/kg, SC, BID) on the development of tolerance to the cataleptic effects of haloperidol in mice was borderline and not statistically significant. YPLG increased the in vitro binding of 3H-apomorphine to rat striatal receptors.
The effects of the peptide YPLG on tolerance development and apomorphine binding are similar to those previously reported for MIF-1 and demonstrate that amidation at the carboxyl terminus is not required for biological activity.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)NCC(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPBBBP0008
[D2]	DFBPMUFU0762

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Mycroft FJ, Bhargava HN, Wei ET. Pharmacological activities of the MIF-1 analogues Pro-Leu-Gly, Tyr-Pro-Leu-Gly and pareptide. Peptides. 1987 Nov-Dec;8(6):1051-5. PMID: 2894644 DOI: 10.1016/0196-9781(87)90135-5
Other literature(s)	N.D
PubDate	1987